SHMT1 / SHMT antibody
Principal name
SHMT1 / SHMT antibody
Alternative names for SHMT1 / SHMT antibody
Serine methylase, Glycine hydroxymethyltransferase, Serine hydroxymethyltransferase cytosolic
SwissProt ID
P07511 (Rabit), P34896 (Human), P35623 (Sheep), P37291 (Yeast), P50431 (Mouse), P50432 (Caeel), Q5E9P9 (Bovin)
Gene ID
6470 (SHMT1)
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair)
Background of SHMT1 / SHMT antibody
- Kit Component:
- - KN203461G1, SHMT1 gRNA vector 1 in pCas-Guide vector
- KN203461G2, SHMT1 gRNA vector 2 in pCas-Guide vector
- KN203461D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Herbig K., et al. (2002) J Biol Chem. 277:38381-38389.Trivedi V., et al. (2002) J Biol Chem. 277:17161-17169.
Immunoperoxidase of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1 ug/ml]
Immunoperoxidase of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1 ug/ml]
SHMT1 monoclonal antibody (M01), clone 4F9 Western Blot analysis of SHMT1 expression in HeLa ( Cat # L013V1 ).
Western Blot analysis of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody (M01), clone 4F9.Lane 1: SHMT1 transfected lysate(53.1 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged SHMT1 is approximately 1ng/ml as a capture antibody.
SHMT1 polyclonal antibody (A01), Lot # 051213JC01 Western Blot analysis of SHMT1 expression in HepG2 ( Cat # L019V1 ).
SHMT1 MaxPab polyclonal antibody. Western Blot analysis of SHMT1 expression in human liver.
Western Blot analysis of SHMT1 expression in transfected 293T cell line (H00006470-T01) by SHMT1 MaxPab polyclonal antibody.Lane 1: SHMT1 transfected lysate(53.13 KDa).Lane 2: Non-transfected lysate.
SHMT1 MaxPab rabbit polyclonal antibody. Western Blot analysis of SHMT1 expression in human liver.
Western Blot analysis of SHMT1 expression in transfected 293T cell line (H00006470-T01) by SHMT1 MaxPab polyclonal antibody.Lane 1: SHMT1 transfected lysate(53.10 KDa).Lane 2: Non-transfected lysate.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody clone 4F9.
SHMT1 monoclonal antibody clone 4F9 Western Blot analysis of SHMT1 expression in HeLa.
Detection limit for recombinant GST tagged SHMT1 is approximately 1 ng/ml as a capture antibody.
WB Suggested Anti-SHMT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: A549 cell lysateSHMT1 is supported by BioGPS gene expression data to be expressed in A549
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM20941PU-N | SHMT1 / SHMT (374-483) antibody |
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 |
Mouse | IgG1 | 4F9 | Purified | Hu | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
 +5 additional images |
Highly validated antibody! |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303461 | SHMT1 / SHMT (transcript variant 1) |
||||
| Recombinant protein of human serine hydroxymethyltransferase 1 (soluble) (SHMT1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP305102 | SHMT1 / SHMT (transcript variant 2) |
||||
| Recombinant protein of human serine hydroxymethyltransferase 1 (soluble) (SHMT1), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401341 | SHMT1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY407729 | SHMT1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |