SIAH2 antibody
Principal name
SIAH2 antibody
Alternative names for SIAH2 antibody
Siah-2, E3 ubiquitin-protein ligase SIAH2, Seven in absentia homolog 2
Ncbi ID
9606, NP_005058, NP_005058.3, NM_005067, NP_033200.2, NM_009174, NM_134457
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Western blot / Immunoblot (WB)
Background of SIAH2 antibody
SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkappaB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.
General readings
Fiucci G. et al. Siah-1b is a direct transcriptional target of p53: identification of the functional p53 responsive element in the siah-1b promoter.Proc Natl Acad Sci U S A. 2004;101(10):3510-5.
Immunohistochemical staining of SIAH2 on human colorectal adenocarcinoma with SIAH2 monoclonal antibody, clone 24E6H3 ( Cat # MAB2298 ). Staining of SIAH was not observed in normal colon epithelium. Photos courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center.
Immunofluorescence staining of SIAH2 on NIH : OVCAR-3 with SIAH2 monoclonal antibody, clone 24E6H3 ( Cat # MAB2298 ).
Western blot analysis of 293T cells overexpressing human SIAH2 with MG-132 ( protease inhibitor ) , using SIAH2 polyclonal antibody ( Cat # PAB8469 ) .
Human 721_B; Host:Rabbit. Target Name:SIAH2. Sample Tissue:721_B. Antibody Dilution: 1.0ug/ml. SIAH2 is supported by BioGPS gene expression data to be expressed in 721_B.; SIAH2 antibody - middle region (ARP33290_P050) in Human 721_B cells using Western Blot
Human Muscle; WB Suggested Anti-SIAH2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle; SIAH2 antibody - middle region (ARP33290_P050) in Human Muscle cells using Western Blot
Human HepG2; Host: Rabbit . Target Name: SIAH2 . Sample Tissue: HepG2 . Antibody Dilution: 1.0ug/ml. SIAH2 is supported by BioGPS gene expression data to be expressed in HepG2.; SIAH2 antibody - N-terminal region (ARP58071_P050) in Human HepG2 cells using Western Blot
Human MCF7; Host: Rabbit . Target Name: SIAH2 . Sample Tissue: MCF7 . Antibody Dilution: 1.0ug/ml. SIAH2 is supported by BioGPS gene expression data to be expressed in MCF7.; SIAH2 antibody - N-terminal region (ARP58071_P050) in Human MCF7 cells using Western Blot
Hela; Host: Rabbit. Target Name:SIAH2. Sample Tissue:Hela. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1ug/ml. Peptide Concentration: 5ug/ml. Lysate Quantity: 25ug/lane/lane. Gel Concentration: 0.12.; SIAH2 antibody - N-terminal region (ARP58071_P050) in Hela cells using Western Blot
Human; . Lanes: 1: 50ug HEK293T lysate, 2: 50ug A549 lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:5000. Gene Name: SIAH2. Submitted by: Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University, Jena.; SIAH2 antibody - N-terminal region (ARP58071_P050) in Human cells using Western Blot
Human HeLa; WB Suggested Anti-SIAH2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate; SIAH2 antibody - N-terminal region (ARP58071_P050) in Human HeLa cells using Western Blot
Immunohistochemistry (IHC) analyzes of Siah-2 antibody (Cat.-No.: AP20493PU-N) in paraffin-embedded human heart tissue.
Western blot (WB) analysis of Siah-2 antibody (Cat.-No.: AP20493PU-N) in extracts from COLO cells.
Immunohistochemical analysis of SIAH2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of SIAH2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of SIAH2 expression in MCF7 (A) whole cell lysates.
Western blot analysis of extracts from COLO cells, using SIAH2 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-SIAH2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Muscle
Host:Rabbit; Target Name:SIAH2; Sample Tissue:721_B; Antibody Dilution: 1.0 ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in 721_B
Immunohistochemistry: SIAH2 Antibody (24E6H3) [TA336562] - Staining of SIAH in human colorectal adenocarcinoma using TA336562. Staining of SIAH was not observed in normal colon epithelium. Photos courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center.
Immunocytochemistry/Immunofluorescence: SIAH2 Antibody (24E6H3) [TA336562] - Immunofluorescent staining of SIAH in ovarian carcinoma (OVCR3)cells using TA336562.
Lanes: 1: 50ug HEK293T lysate, 2: 50ug A549 lysate; Primary Antibody Dilution: 1:1000; Secondary Antibody: Anti-rabbit-HRP; Secondary Antibody Dilution: 1:5000; Gene Name: SIAH2; Submitted by: Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology
WB Suggested Anti-SIAH2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Hela cell lysate
Host: Rabbit; Target Name: SIAH2; Sample Tissue: HepG2; Antibody Dilution: 1.0 ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in HepG2
Host: Rabbit; Target Name: SIAH2; Sample Tissue: MCF7; Antibody Dilution: 1.0 ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in MCF7
Host: Rabbit; Target Name:SIAH2; Sample Tissue:Hela; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide ; Primary Antibody Concentration:1 ug/ml; Peptide Concentration: 5ug/ml; Lysate Quantity: 25ug/lane/lane; Gel Concentration: 0.12
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (SIAH2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (SIAH2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
Lysates
| Catalog No. | ||
|---|---|---|
| LY417551 | SIAH2 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
