Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » SIK1 antibody

SIK1 antibody

(reset)

Principal name

SIK1 antibody

Alternative names for SIK1 antibody

SIK-1, SIK, SNF1LK, Serine/threonine-protein kinase SIK1, Salt-inducible protein kinase 1, Serine/threonine-protein kinase SNF1-like kinase 1

SwissProt ID

P57059 (Human), Q60670 (Mouse), Q9R1U5 (Rat)

Gene ID

102724428 (LOC102724428), 59329 (Sik1), 150094 (SIK1), 17691

Ncbi ID

9606, NP_775490, NP_775490.2, NM_021693, NM_173354, NP_034961.2, NM_010831

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Dros (Drosophila), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SIK1 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

General readings

Nishimura,Y., (1999) Biochem. Biophys. Res. Commun. 254 (1), 21-26

PAB3316 - SIK1
Formalin-fixed and paraffin-embedded human cancer tissue reacted with SNF1LK polyclonal antibody ( Cat # PAB3316 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
PAB3316 - SIK1
The SNF1LK polyclonal antibody ( Cat # PAB3316 ) is used in Western blot to detect SNF1LK in T-47D cell lysate.
H00150094-B01P - SIK1
Immunofluorescence of purified MaxPab antibody to SNF1LK on HeLa cell. [antibody concentration 10 ug/ml]
H00150094-B01P - SIK1
Western Blot analysis of SIK1 expression in transfected 293T cell line (H00150094-T01) by SIK1 MaxPab polyclonal antibody.Lane 1: SNF1LK transfected lysate(86.13 KDa).Lane 2: Non-transfected lysate.
ARP45918_T100 - SIK1
Human Stomach; SNF1LK antibody - N-terminal region (ARP45918_T100) in Human Stomach cells using Immunohistochemistry
ARP45918_T100 - SIK1
Human Jurkat; WB Suggested Anti-SNF1LK Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate; SNF1LK antibody - N-terminal region (ARP45918_T100) in Human Jurkat cells using Western Blot
AP15056PU-N - SIK1
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP15056PU-N - SIK1
The anti-SNF1LK Pab is used in Western blot to detect SNF1LK in T-47D cell lysate
H00150094-D01P - SIK1
SIK1 MaxPab rabbit polyclonal antibody. Western Blot analysis of SIK1 expression in mouse lung.
H00150094-D01P - SIK1
SIK1 MaxPab rabbit polyclonal antibody. Western Blot analysis of SIK1 expression in human kidney.
H00150094-D01P - SIK1
Western Blot analysis of SIK1 expression in transfected 293T cell line (H00150094-T02) by SIK1 MaxPab polyclonal antibody.Lane 1: SNF1LK transfected lysate(84.90 KDa).Lane 2: Non-transfected lysate.
B8185-1 - SIK1
Western blot analysis of extracts from COLO205 cells, treated with PMA 125ng/ml 30', using SIK (Ab-182) Antibody. The lane on the right is treated with the synthesized peptide.
ARP45917_P050 - SIK1
Rat Lung; WB Suggested Anti-Sik1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Rat Lung; Sik1 antibody - middle region (ARP45917_P050) in Rat Lung cells using Western Blot
AM31899PU-N - SIK1
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31899PU-N - SIK1
Detection limit for recombinant GST tagged SIK1 is 0.3 ng/ml as a capture antibody.
TA316162 - SIK1
Western blot analysis of extracts from COLO cells, treated with PMA (125ng/ml, 30mins), using SIK (Ab-182) antibody.The lane on the right is treated with the synthesized peptide.
TA320117 - SIK1
Western blot analysis of SIK1 in human small intestine tissue lysate with SIK1 antibody at 1 ug/mL.
TA320117 - SIK1
Immunohistochemistry of SIK1 in human small intestine tissue with SIK1 antibody at 5 ug/mL.
TA338957 - SIK1
WB Suggested Anti-SNF1LK Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate
TA338957 - SIK1
Human Stomach
TA351732 - SIK1
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: HT29 cells, hela cells, mouse liver tissue, A549 cells, Jurkat cells, Primary antibody: (SIK1 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
TA351732 - SIK1
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (SIK1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)

14 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIK1 antibody
  Rabbit polyclonal SIK (Ab-182) antibody  
Western blot analysis of extracts from COLO cells, treated with PMA (125ng/ml, 30mins), using SIK (Ab-182) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

SIK1 (Center) antibody
  Rabbit Polyclonal SIK1 Antibody  
Western blot analysis of SIK1 in human small intestine tissue lysate with SIK1 antibody at 1 ug/mL. Rabbit Aff - Purified Hu E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+1 additional image

SIK1 antibody
  Rabbit Polyclonal Anti-SIK1 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: HT29 cells, hela cells, mouse liver tissue, A549 cells, Jurkat cells, Primary antibody: (SIK1 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

SIK1 (1-101) antibody
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2C12 Purified Hu E, P
50 µg / €475.00
  OriGene Technologies GmbH
+1 additional image

SIK1 (C-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

SIK1 - human gene knockout kit via CRISPR
SIK1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIK1 - human gene knockout kit via CRISPR
SIK1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIK1 - human gene knockout kit via CRISPR
SIK1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIK1 - human gene knockout kit via CRISPR
SIK1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

SIK1 overexpression lysate
SIK1 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Sik1 (GFP-tagged) - Mouse salt inducible kinase 1 (Sik1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Sik1 (GFP-tagged) - Mouse salt inducible kinase 1 (Sik1), 200 uL, >10^7 TU/mL  
200 µl / €1,580.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Sik1 (Myc-DDK-tagged) - Mouse salt inducible kinase 1 (Sik1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Sik1 (Myc-DDK-tagged) - Mouse salt inducible kinase 1 (Sik1), 200 uL, >10^7 TU/mL  
200 µl / €1,420.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Sik1 (GFP-tagged ORF) - Rat SNF1-like kinase (Snf1lk), 200 uL, >10^7 TU/mL
Lenti ORF particles, Sik1 (GFP-tagged ORF) - Rat SNF1-like kinase (Snf1lk), 200 uL, >10^7 TU/mL  
200 µl / €1,570.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Sik1 (Myc-DDK-tagged ORF) - Rat SNF1-like kinase (Snf1lk), 200 uL, >10^7 TU/mL
Lenti ORF particles, Sik1 (Myc-DDK-tagged ORF) - Rat SNF1-like kinase (Snf1lk), 200 uL, >10^7 TU/mL  
200 µl / €1,410.00
  OriGene Technologies, Inc.
 

14 Item(s)

per page
14 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. CRISPR (4)
  2. Lysate (1)
  3. Mouse Lenti ORF Particle (2)
  4. Primary Antibody (5)
  5. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (4)
  2. Monoclonal (1)
Target
  1. SIK1 (5)
Property
  1. 1-101 (1)
  2. C-term (1)
  3. Center (1)
Reactivity
  1. Human (Hu) (5)
  2. Mouse (Ms) (1)
  3. Rat (Rt) (1)
Applications
  1. Enzyme Immunoassay (E) (4)
  2. Western blot / Immunoblot (WB) (4)
  3. Paraffin Sections (P) (3)
Presentation
  1. Purified (4)
  2. Aff - Purified (1)
Host
  1. Rabbit (4)
  2. Mouse (1)
Images
  1. 1 image (5)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. IHC (2)
  3. Paraffin Sections (P) (2)
  4. ELISA (capture) (E(capture)) (1)
Species (CRISPR)
  1. Human (4)
Donor Vector
  1. GFP-Puro (1)
  2. Luciferase-Puro (1)
  3. RFP-BSD (1)
  4. mBFP-Neo (1)
Manufacturer
  1. OriGene Technologies (14)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn