SIN3B antibody
Principal name
SIN3B antibody
Alternative names for SIN3B antibody
KIAA0700, Paired amphipathic helix protein Sin3b, Transcriptional corepressor Sin3b, Histone deacetylase complex subunit Sin3b
Ncbi ID
NP_033214, 9606, NP_056075, NP_056075.1, NM_015260, NP_001106719.1, NM_009188, NM_001113248
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of SIN3B antibody
- Kit Component:
- - KN216859G1, SIN3B gRNA vector 1 in pCas-Guide vector
- KN216859G2, SIN3B gRNA vector 2 in pCas-Guide vector
- KN216859D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Nomura,M., et al., (2005) J. Mol. Biol. 354 (4), 903-915
Mouse SP2/0; WB Suggested Anti-SIN3B Antibody Titration: 2.5ug/ml. ELISA Titer: 1:1562500. Positive Control: SP2/0 cell lysate; SIN3B antibody - N-terminal region (ARP36976_T100) in Mouse SP2/0 cells using Western Blot
Western Blot analysis of SIN3B expression in transfected 293T cell line (H00023309-T01) by SIN3B MaxPab polyclonal antibody.Lane 1: SIN3B transfected lysate(38.83 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SIN3B expression in transfected 293T cell line (H00023309-T01) by SIN3B MaxPab polyclonal antibody.Lane 1: SIN3B transfected lysate(38.83 KDa).Lane 2: Non-transfected lysate.
Human Thymus; WB Suggested Anti-Sin3b Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Thymus; Sin3b antibody - N-terminal region (ARP36977_P050) in Human Thymus cells using Western Blot
Hum. Fetal Brain; Host: Rabbit . Target Name: SIN3B . Sample Tissue: Human Fetal Brain . Antibody Dilution: 1.0ug/ml.; SIN3B antibody - middle region (ARP50589_P050) in Hum. Fetal Brain cells using Western Blot
Human Brain; WB Suggested Anti-SIN3B Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; SIN3B antibody - middle region (ARP50589_P050) in Human Brain cells using Western Blot
Human HCT15; WB Suggested Anti-SIN3B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HCT15 cell lysate; SIN3B antibody - middle region (ARP50590_P050) in Human HCT15 cells using Western Blot
Immunofluorescent analysis of SIN3B staining in HCT116 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of SIN3B expression in HCT116 (A) whole cell lysates.
Western blot analysis of extracts from A549 cells, using SIN3B antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using SIN3B antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-SIN3B Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain
Host: Rabbit; Target Name: SIN3B; Sample Tissue: Human Fetal Brain; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-SIN3B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HCT15 cell lysate
WB Suggested Anti-SIN3B Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:1562500; Positive Control: SP2/0 cell lysate
WB Suggested Anti-Sin3b Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Thymus
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315238 | SIN3B antibody |
||||||
| Rabbit polyclonal anti-SIN3B antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY414651 | SIN3B overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC216859L2V | Lenti ORF particles, SIN3B (mGFP-tagged)-Human SIN3 homolog B, transcription regulator (yeast) (SIN3B), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,430.00
|
|
| OriGene Technologies, Inc. | ||
| RC216859L1V | Lenti ORF particles, SIN3B (Myc-DDK-tagged)-Human SIN3 homolog B, transcription regulator (yeast) (SIN3B), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,200.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR220995L2V | Lenti ORF particles, Sin3b (GFP-tagged) - Mouse transcriptional regulator, SIN3B (yeast) (Sin3b), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,030.00
|
|
| OriGene Technologies, Inc. | ||
| MR220996L2V | Lenti ORF particles, Sin3b (GFP-tagged) - Mouse transcriptional regulator, SIN3B (yeast) (Sin3b), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR220995L1V | Lenti ORF particles, Sin3b (Myc-DDK-tagged) - Mouse transcriptional regulator, SIN3B (yeast) (Sin3b), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,830.00
|
|
| OriGene Technologies, Inc. | ||
| MR220996L1V | Lenti ORF particles, Sin3b (Myc-DDK-tagged) - Mouse transcriptional regulator, SIN3B (yeast) (Sin3b), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||