SIRT5 antibody
Principal name
SIRT5 antibody
Alternative names for SIRT5 antibody
SIRT-5, SIR2-like protein 5, SIR2L5, sirtuin type 5, sirtuin 5, sirtuin-5, sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae), NAD-dependent deacetylase sirtuin-5
Ncbi ID
NP_112534, 9606, NP_036373.1, NP_036373, NM_031244, NM_001193267, NP_849179.1, NM_178848, NM_012241
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of SIRT5 antibody
SIRT5 is included in class III of the sirtuin family which ischaracterized by a sirtuin core domain. Human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in two transcript variants.
General readings
Zhou,Y., et al. (2012) J. Biol. Chem. 287 (34), 28307-28314
Fischer,F., et al. (2012) PLoS ONE 7 (9), E45098
Human Heart; WB Suggested Anti-SIRT5 Antibody Titration: 5.0ug/ml. ELISA Titer: 1:62500. Positive Control: Human heart; SIRT5 antibody - C-terminal region (ARP40125_T100) in Human Heart cells using Western Blot
Human HEK293T; . Lanes: Lane1: Untransfected HEK293T lysate. Lane2: mSIRT5-FLAG transfected HEK293T lysate. Primary Antibody Dilution: 1:200. Secondary Antibody: ProteinA-HRP. Secondary Antibody Dilution: 1:5000. Gene Name: SIRT5. Submitted by: Anonymous. There is BioGPS gene expression data showing that SIRT5 is expressed in HEK293T.; SIRT5 antibody - C-terminal region (ARP40125_T100) in Human HEK293T cells using Western Blot
Human kidney; SIRT5 antibody - middle region (ARP32391_T100) in Human kidney cells using Immunohistochemistry
Human Lung; SIRT5 antibody - middle region (ARP32391_T100) in Human Lung cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-SIRT5 Antibody Titration: 1.0-2.0ug/ml. Positive Control: Jurkat cell lysate; SIRT5 antibody - middle region (ARP32391_T100) in Human Jurkat cells using Western Blot
Human Intestine; SIRT5 antibody - C-terminal region (ARP32390_P050) in Human Intestine cells using Immunohistochemistry
Human Spermatophore; SIRT5 antibody - C-terminal region (ARP32390_P050) in Human Spermatophore cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-SIRT5 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; SIRT5 antibody - C-terminal region (ARP32390_P050) in Human HepG2 cells using Western Blot
Western blot analysis of SIRT5 in A ) human, B ) mouse and C ) rat intestine cell lysate. Using SIRT5 polyclonal antibody ( Cat # PAB0206 ) at 2 µg/ml.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with SIRT5 polyclonal antibody ( Cat # PAB4825 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Western blot analysis of SIRT5 polyclonal antibody ( Cat # PAB4825 ) in Jurkat cell lysate ( 35 µg/lane ) . SIRT5 ( arrow ) was detected using the purified polyclonal antibody.
Western Blot analysis of SIRT5 expression in transfected 293T cell line (H00023408-T01) by SIRT5 MaxPab polyclonal antibody.Lane 1: SIRT5 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-SIRT5 antibody (cat#AP13218PU-N) in Jurkat cell line lysate (35ug/lane). SIRT5(arrow) was detected using the purified Pab.
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
Human heart; WB Suggested Anti-SIRT5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human heart; SIRT5 antibody - middle region (ARP40126_P050) in Human heart cells using Western Blot
Western Blot analysis of SIRT5 expression in transfected 293T cell line (H00023408-T01) by SIRT5 MaxPab polyclonal antibody.Lane 1: SIRT5 transfected lysate(33.90 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SIRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503232)
Anti-SIRT5 mouse monoclonal antibody (TA503232) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody.
HEK293T cells transfected with either RC200189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SIRT5 antibody(TA503232), and then analyzed by flow cytometry.
Anti-SIRT5 mouse monoclonal antibody (TA503233) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.
HEK293T cells transfected with either RC200189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SIRT5 antibody(TA503233), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.
HEK293T cells transfected with either RC200189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SIRT5 antibody(TA503279), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-SIRT5 antibody(TA503279),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-SIRT5 antibody(TA503279),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-SIRT5 mouse monoclonal antibody (TA503280) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.
HEK293T cells transfected with either RC200189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SIRT5 antibody(TA503280), and then analyzed by flow cytometry.
Immunofluorescent staining of HeLa cells using anti-SIRT5 mouse monoclonal antibody (TA503281).
Anti-SIRT5 mouse monoclonal antibody (TA503281) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody.
HEK293T cells transfected with either RC200189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SIRT5 antibody(TA503281), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SIRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503282)
Immunofluorescent staining of HeLa cells using anti-SIRT5 mouse monoclonal antibody (TA503282).
Anti-SIRT5 mouse monoclonal antibody (TA503282) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.
HEK293T cells transfected with either RC200189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SIRT5 antibody(TA503282), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.
HEK293T cells transfected with either RC200189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SIRT5 antibody(TA503283), and then analyzed by flow cytometry.
Anti-SIRT5 mouse monoclonal antibody (TA503292) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.
HEK293T cells transfected with either RC200189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SIRT5 antibody(TA503292), and then analyzed by flow cytometry.
Anti-SIRT5 mouse monoclonal antibody (TA503315) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.
HEK293T cells transfected with either RC200189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SIRT5 antibody(TA503315), and then analyzed by flow cytometry.
Western blot analysis of SIRT5 in human liver tissue lysate with SIRT5 antibody at 1 ug/mL.
Predicted band size: 22 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
Predicted band size: 22 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using SIRT5 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western Blot: SIRT5 Antibody [TA336344] - Analysis of SIRT5 in A) human, B) mouse and C) rat intestine cell lysate using this antibody at 2 ug/ml.
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503232 | SIRT5 antibody |
||||||
| SIRT5 mouse monoclonal antibody, clone OTI2F1 (formerly 2F1) | |||||||
 |
Mouse | IgG1 | OTI2F1 | Purified | Hu, Rt | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
||||||
| TA503279 | SIRT5 antibody |
||||||
| SIRT5 mouse monoclonal antibody, clone OTI 1G9 (formerly 1G9) | |||||||
 |
Mouse | IgG2b | OTI 1G9 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
| TA503281 | SIRT5 antibody |
||||||
| SIRT5 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6) | |||||||
 |
Mouse | IgG1 | OTI1A6 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
| TA503282 | SIRT5 antibody |
||||||
| SIRT5 mouse monoclonal antibody, clone OTI2A11 (formerly 2A11) | |||||||
 |
Mouse | IgG1 | OTI2A11 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
| TA319855 | SIRT5 (C-term) antibody |
||||||
| Chicken Polyclonal SIRT5 Antibody | |||||||
 |
Chicken | Aff - Purified | Hu, Ms | E, WB |
0.1 mg /
€415.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA324179 | SIRT5 antibody |
||||||
| Anti-SIRT5 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA324180 | SIRT5 antibody |
||||||
| Anti-SIRT5 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA336344 | SIRT5 antibody |
||||||
| Rabbit Polyclonal Sirtuin 5/SIRT5 Antibody | |||||||
![Western Blot: SIRT5 Antibody [TA336344] - Analysis of SIRT5 in A) human, B) mouse and C) rat intestine cell lysate using this antibody at 2 ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB100-56372-1-W.jpg) |
Rabbit | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€395.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA503233 | SIRT5 antibody |
||||||
| SIRT5 mouse monoclonal antibody, clone OTI2C4 (formerly 2C4) | |||||||
 |
Mouse | IgG1 | OTI2C4 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA503280 | SIRT5 antibody |
||||||
| SIRT5 mouse monoclonal antibody, clone OTI 1G5 (formerly 1G5) | |||||||
 |
Mouse | IgG1 | OTI 1G5 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA503283 | SIRT5 antibody |
||||||
| SIRT5 mouse monoclonal antibody, clone OTI2E10 (formerly 2E10) | |||||||
 |
Mouse | IgG2a | OTI2E10 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA503292 | SIRT5 antibody |
||||||
| SIRT5 mouse monoclonal antibody, clone OTI 2E12 (formerly 2E12) | |||||||
 |
Mouse | IgG1 | OTI 2E12 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA503315 | SIRT5 antibody |
||||||
| SIRT5 mouse monoclonal antibody, clone OTI1B6 (formerly 1B6) | |||||||
 |
Mouse | IgG1 | OTI1B6 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| AP08461PU-N | SIRT5 (30-46) antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | P, WB |
50 µg /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| AP13218PU-N | SIRT5 (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300189 | SIRT5 (transcript variant 1) |
||||
| Recombinant protein of human sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae) (SIRT5), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY410600 | SIRT5 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY429775 | SIRT5 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. | ||
| LY434135 | SIRT5 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR204397L2V | Lenti ORF particles, Sirt5 (GFP-tagged) - Mouse sirtuin 5 (silent mating type information regulation 2 homolog) 5 (S. cerevisiae) (Sirt5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
