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SIRT6 antibody

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Principal name

SIRT6 antibody

Alternative names for SIRT6 antibody

SIRT-6, SIR2-like protein 6, SIR2L6, sirtuin type 6, sirtuin 6, sirtuin-6, sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae), NAD-dependent deacetylase sirtuin-6

SwissProt ID

P59941 (Mouse), Q8N6T7 (Human), Q9NRC7 (Human)

Gene ID

51548 (SIRT6), 50721 (Sirt6)

Ncbi ID

NP_057623, NP_001180214.1, 9606, 10090, NP_001180214, AAH28220.1, NM_016539, NP_853617.1, NM_181586, NM_001163430, NM_001193285

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), African clawed frog

Available hosts

Rabbit, Mouse, Chicken

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of SIRT6 antibody

SIRT6 is a D-dependent protein deacetylase. SIRT6 has deacetylase activity towards 'Lys-9' and 'Lys-56' of histone H3. SIRT6 modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates 'Lys-9' of histone H3 at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Deacetylation of nucleosomes interferes with RELA binding to target D. SIRT6 may be required for the association of WRN with telomeres during S-phase and for normal telomere maintence. SIRT6 is required for genomic stability, normal IGF1 serum levels and normal glucose homeostasis. SIRT6 modulates cellular senescence and apoptosis. SIRT6 regulates the production of TNF protein.This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombition of rD. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.

General readings

Michishita E., et al. (2008) Nature. 27;452(7186):492-6.
Mostoslavsky R., et al. (2006) Cell. 27;124(2):315-29.

ARP32409_T100 - SIRT6
Human U2OS; U2OS; SIRT6 antibody - N-terminal region (ARP32409_T100) in Human U2OS cells using Western Blot
ARP32409_T100 - SIRT6
Human HepG2; WB Suggested Anti-SIRT6 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate; SIRT6 antibody - N-terminal region (ARP32409_T100) in Human HepG2 cells using Western Blot
AP07972PU-N - SIRT6
Figure 1. Staining SIRT6 in Adrenal by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
PAB1985 - SIRT6
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with SIRT6 polyclonal antibody ( Cat # PAB1985 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AR09083PU-L - SIRT6
AR09083PU-L - SIRT6
SIRT6, His-tagged: 15% SDS-PAGE
AP13219PU-N - SIRT6
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP13219PU-N - SIRT6
Western blot analysis of SIRT6 Antibody (C-term) in mouse stomach tissue lysates (35ug/lane). SIRT6 (arrow) was detected using the purified Pab.
H00051548-B01P - SIRT6
Western Blot analysis of SIRT6 expression in transfected 293T cell line (H00051548-T02) by SIRT6 MaxPab polyclonal antibody.Lane 1: SIRT6 transfected lysate(36.08 KDa).Lane 2: Non-transfected lysate.
PAB12035 - SIRT6
Western blot analysis of Sirt6 in MEF lysate with Sirt6 polyclonal antibody ( Cat# PAB12035 ).
PAB12036 - SIRT6
Western blot analysis of SIRT6 in HeLa whole cell lysate with SIRT6 polyclonal antibody ( Cat # PAB12036 ).
PAB12037 - SIRT6
Western blot analysis of SIRT6 in HeLa whole cell lysate with SIRT6 polyclonal antibody ( Cat# PAB12037 ).
ARP32408_P050 - SIRT6
Human U2OS; U2OS; SIRT6 antibody - middle region (ARP32408_P050) in Human U2OS cells using Western Blot
ARP32408_P050 - SIRT6
Human Spleen; WB Suggested Anti-SIRT6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Spleen; SIRT6 antibody - middle region (ARP32408_P050) in Human Spleen cells using Western Blot
TA503670 - SIRT6
Anti-SIRT6 mouse monoclonal antibody (TA503670) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT6(RC202833).
TA503670 - SIRT6
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT6 (RC202833, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT6.
TP302833 - SIRT6
TA301523 - SIRT6
Detection of SIRT6 in HeLa whole cell lysate
TA301524 - SIRT6
Detection of SIRT6 in HeLa whole cell lysate
TA301524 - SIRT6
Immunocytochemistry/Immunofluorescence: SIRT6 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
TA319931 - SIRT6
Western blot analysis of SIRT6 in HeLa cell lysate with SIRT6 antibody at (A) 0.5 and (B) 1 ug/mL.
TA324068 - SIRT6
Predicted band size: 39 kDa . Positive control: Mouse stomach tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds)
TA330290 - SIRT6
WB Suggested Anti-SIRT6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Spleen
TA330290 - SIRT6
U2OS
TA330291 - SIRT6
WB Suggested Anti-SIRT6 Antibody Titration: 1.25ug/ml; Positive Control: HepG2 cell lysate
TA330291 - SIRT6
U2OS
TA336289 - SIRT6
Western Blot: SIRT6 Antibody [TA336289] - WB analysis of SIRT6 in NIH/3T3 cell lysate.
TA336289 - SIRT6
Immunohistochemistry: SIRT6 Antibody [TA336289] - SIRT6 antibody was tested in mouse testis using DAB with hematoxylin counterstain.
TA347046 - SIRT6
Western blot analysis of extracts from CHO-K1 cells,transfected with pcDNA3.1-FLAG vector (A) or transfected with pcDNA3.1-FLAG-SIRT6 construct (B),using SIRT6 mouse mAb (1:500 diluted).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIRT6 antibody
  SIRT6 mouse monoclonal antibody, clone OTI1G3 (formerly 1G3)  
Anti-SIRT6 mouse monoclonal antibody (TA503670) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT6(RC202833). Mouse IgG1 OTI1G3 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.
+1 additional image

Top Selling product!

TrueMAB

SIRT6 antibody
  Rabbit Polyclonal Antibody against SIRT6  
Detection of SIRT6 in HeLa whole cell lysate Rabbit IgG Purified Hu WB
0.1 ml / €415.00
  OriGene Technologies, Inc.

SIRT6 antibody
  Rabbit Polyclonal Antibody against SIRT6  
Detection of SIRT6 in HeLa whole cell lysate Rabbit IgG Purified Hu WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

SIRT6 (C-term) antibody
  Chicken Polyclonal SIRT6 Antibody  
Western blot analysis of SIRT6 in HeLa cell lysate with SIRT6 antibody at (A) 0.5 and (B) 1 ug/mL. Chicken Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.

SIRT6 antibody
  Anti-SIRT6 Rabbit Polyclonal Antibody  
Predicted band size: 39 kDa . Positive control: Mouse stomach tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

SIRT6 antibody
  Rabbit Polyclonal SIRT6 Antibody  
Western Blot: SIRT6 Antibody [TA336289] - WB analysis of SIRT6 in NIH/3T3 cell lysate. Rabbit Purified Ms C, P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

SIRT6 antibody
  Mouse Monoclonal SIRT6 Antibody  
Western blot analysis of extracts from CHO-K1 cells,transfected with pcDNA3.1-FLAG vector (A) or transfected with pcDNA3.1-FLAG-SIRT6 construct (B),using SIRT6 mouse mAb (1:500 diluted). Mouse IgG1 1B5-G10-H9 Purified WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

SIRT6 (305-320) antibody
Figure 1. Staining SIRT6 in Adrenal by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu E, P
50 µg / €425.00
  OriGene Technologies GmbH

SIRT6 (C-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

SIRT6 - human gene knockout kit via CRISPR
SIRT6 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIRT6 - human gene knockout kit via CRISPR
SIRT6 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIRT6 - human gene knockout kit via CRISPR
SIRT6 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIRT6 - human gene knockout kit via CRISPR
SIRT6 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sirt6 - mouse gene knockout kit via CRISPR
Sirt6 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sirt6 - mouse gene knockout kit via CRISPR
Sirt6 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sirt6 - mouse gene knockout kit via CRISPR
Sirt6 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sirt6 - mouse gene knockout kit via CRISPR
Sirt6 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SIRT6
  Recombinant protein of human sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) (SIRT6)  
SIRT6 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

SIRT6 overexpression lysate
SIRT6 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SIRT6 (Myc-DDK-tagged)-Human sirtuin 6 (SIRT6), transcript variant 2, 200 uL, >10^7 TU/mL
Lenti ORF particles, SIRT6 (Myc-DDK-tagged)-Human sirtuin 6 (SIRT6), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €910.00
  OriGene Technologies, Inc.
 

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22 products - FILTER YOUR RESULTS!

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