SIRT7 antibody
Principal name
SIRT7 antibody
Alternative names for SIRT7 antibody
SIRT-7, SIR2-like protein 7, SIR2L7, sirtuin type 7, sirtuin 7, sirtuin-7, sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 7, NAD-dependent deacetylase sirtuin-7
Ncbi ID
NP_057622, 9606, NP_057622.1, NM_016538, NP_694696.2, NM_153056
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of SIRT7 antibody
SIRT7 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombition of rD. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. SIRT7 is included in class IV of the sirtuin family. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombition of rD. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
General readings
Mahon,P.C., et al., (2000) Biochem. Biophys. Res. Commun. 273 (2), 793-798
Human Jurkat; WB Suggested Anti-SIRT7 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; SIRT7 antibody - C-terminal region (ARP32406_P050) in Human Jurkat cells using Western Blot
Western blot analysis of SIRT7 in ( A ) human liver and ( B ) PBMC lysate using SIRT7 polyclonal antibody ( Cat # PAB0197 ) at 1 µg/ml.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with SIRT7 polyclonal antibody ( Cat # PAB4826 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Western blot analysis of SIRT7 polyclonal antibody ( Cat # PAB4826 ) in placenta tissue lysate ( 35 µg/lane ) . SIRT7 ( arrow ) was detected using the purified polyclonal antibody.
Immunofluorescence of purified MaxPab antibody to SIRT7 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of SIRT7 expression in transfected 293T cell line (H00051547-T01) by SIRT7 MaxPab polyclonal antibody.Lane 1: SIRT7 transfected lysate(44 KDa).Lane 2: Non-transfected lysate.
SIRT7 MaxPab polyclonal antibody. Western Blot analysis of SIRT7 expression in HeLa.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of SIRT7 (arrow) using SIRT7 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SIRT7 gene (Lane 2).
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
Immunofluorescence of purified MaxPab antibody to SIRT7 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of SIRT7 expression in transfected 293T cell line (H00051547-T01) by SIRT7 MaxPab polyclonal antibody.Lane 1: SIRT7 transfected lysate(44 KDa).Lane 2: Non-transfected lysate.
SIRT7 MaxPab polyclonal antibody. Western Blot analysis of SIRT7 expression in HeLa.
Western blot analysis of SIRT7 in human liver lysates with SIRT7 polyclonal antibody ( Cat# PAB12032 ).
SIRT7 immunoprecipitated from human liver lysates with SIRT7 polyclonal antibody ( Cat# PAB12034 ) and further Western blotted with ( A ) SIRT7 polyclonal antibody ( Cat# PAB12033 ) ( B ) SIRT7 polyclonal antibody ( Cat# PAB12033 ).
Western blot analysis of SIRT7 in human liver lysates with SIRT7 polyclonal antibody ( Cat# PAB12033 ).
SIRT7 immunoprecipitated from human liver lysates with SIRT7 polyclonal antibody ( Cat# PAB12034 ) and further Western blotted with ( A ) SIRT7 polyclonal antibody ( Cat# PAB12033 ) ( B ) SIRT7 polyclonal antibody ( Cat# PAB12033 ).
SIRT7 immunoprecipitated from human liver lysates with SIRT7 polyclonal antibody ( Cat# PAB12034 ) and further Western blotted with ( A ) SIRT7 polyclonal antibody ( Cat# PAB12033 ) ( B ) SIRT7 polyclonal antibody ( Cat# PAB12033 ).
Western Blot analysis of SIRT7 expression in transfected 293T cell line (H00051547-T02) by SIRT7 MaxPab polyclonal antibody.Lane 1: SIRT7 transfected lysate(44.90 KDa).Lane 2: Non-transfected lysate.
Human SH-SYSY; WB Suggested Anti-SIRT7 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: SH-SYSY cell lysate; SIRT7 antibody - middle region (ARP32405_P050) in Human SH-SYSY cells using Western Blot
Detection of SIRT7 in human liver lysates using TA309859.
SIRT7 Immunoprecipitated from human liver lysates with A). TA309859 B). TA309860
Detection of SIRT7 in human liver lysates using TA309860.
SIRT7 Immunoprecipitated from human liver lysates with A). TA309859 B). TA309860
Western blot analysis of SIRT7 in 293 cell lysate with SIRT7 antibody at (A) 0.5 and (B) 1 ug/mL.
Predicted band size: 45 kDa . Positive control: Mouse liver tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds)
Western blot analysis of 293T cell and Hela cell lysate using SIRT7 antibody.
Immunohistochemistry of paraffin-embedded human breast cancer using SIRT7 antibody at dilution of 1:100 (200x lens).
Immunohistochemistry of paraffin-embedded human pancreas using SIRT7 antibody at dilution of 1:100 (200x lens).
WB Suggested Anti-SIRT7 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: SH-SYSY cell lysate
WB Suggested Anti-SIRT7 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate
Western Blot: SIRT7 Antibody [TA336342] - Analysis of SIRT7 in A) human liver and B) PBMC lysate using this antibody at 1 ug/ml.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA309859 | SIRT7 antibody |
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| Rabbit Polyclonal SIRT7 Antibody | |||||||
 |
Rabbit | Purified | Bov, Hu, Ms, Rt | IP, WB |
0.1 ml /
€395.00
|
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA309860 | SIRT7 antibody |
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| Rabbit Polyclonal SIRT7 Antibody | |||||||
 |
Rabbit | Purified | Hu | WB |
0.1 ml /
€395.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA319930 | SIRT7 (N-term) antibody |
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| Chicken Polyclonal SIRT7 Antibody | |||||||
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Chicken | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€415.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA321852 | SIRT7 antibody |
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| Anti-SIRT7 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA326876 | SIRT7 antibody |
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| Rabbit anti-SIRT7 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA336342 | SIRT7 antibody |
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| Rabbit Polyclonal SIRT7 Antibody | |||||||
![Western Blot: SIRT7 Antibody [TA336342] - Analysis of SIRT7 in A) human liver and B) PBMC lysate using this antibody at 1 ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB100-56364-1-W.jpg) |
Rabbit | Purified | Hu | WB |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| AP08462PU-N | SIRT7 (35-51, 361-377) antibody |
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Rabbit | IgG | Aff - Purified | Hu | P, WB |
50 µg /
€425.00
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| OriGene Technologies GmbH | |||||||
| AP13220PU-N | SIRT7 (C-term) antibody |
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Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305658 | SIRT7 |
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| Recombinant protein of human sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae) (SIRT7) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402562 | SIRT7 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203221L2V | Lenti ORF particles, Sirt7 (GFP-tagged) - Mouse sirtuin 7 (silent mating type information regulation 2, homolog) 7 (S. cerevisiae) (Sirt7), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| MR203221L1V | Lenti ORF particles, Sirt7 (Myc-DDK-tagged) - Mouse sirtuin 7 (silent mating type information regulation 2, homolog) 7 (S. cerevisiae) (Sirt7), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||