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SIX5 antibody

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Principal name

SIX5 antibody

Alternative names for SIX5 antibody

DMAHP, Homeobox protein SIX5, Sine oculis homeobox homolog 5, DM locus-associated homeodomain protein

SwissProt ID

P70178 (Mouse), Q8N196 (Human)

Gene ID

147912 (SIX5), 20475 (Six5)

Ncbi ID

NP_787071.2, NM_175875, NP_787071, NP_035513.1

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of SIX5 antibody

Six5 (homeobox protein SIX5), also known as SIX5, BOR2 or DMAHP (DM locus-associated homeodomain protein), is a transcription factor that is expressed in various structures of the adult eye. Localized to the cytoplasm in early development and to the nucleus in the later stages of development,Six5 is involved in regulation of organogenesis and in maintenance of retinal formation. Six5 is able to bind the 5'-TCA[AG][AG]TTNC-3' DNA sequence found in the myogenin and IGFBP5 promoters and, through this binding, can control transcription of the associated mRNA. Six5 is regulated via association with DACH1 (dachshund homolog 1) and is co-activated by the EYA (eyes absent) proteins. Defects in the gene encoding Six5 are the cause of branchiooto- renal syndrome type 2 (BOR2), an autosomal disorder characterized by hearing loss, a deep overbite and myopia. Two isoforms exist due to alternative splicing events.

AP01481PU-N - SIX5
Western blot (WB) analysis of Six5 antibody (Cat.-No.: AP01481PU-N) in extracts from K562 cells.
C16357-1 - SIX5
Western blot analysis of extracts from K562 cells, using SIX5 Antibody. The lane on the right is treated with the synthesized peptide.
TP311844 - SIX5
TA316153 - SIX5
Western blot analysis of extracts from K562 cells, using SIX5 antibody.The lane on the right is treated with the synthesized peptide.
TA324500 - SIX5
SIX5 Antibody (Center) (Cat. #TA324500) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SIX5 antibody detected the SIX5 protein (arrow).
TA324500 - SIX5
SIX5 Antibody (Center) (Cat. #TA324500)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIX5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
TA324500 - SIX5
IF image of Hela cell stained with SIX5 Antibody (Center)(Cat#TA324500).Hela cells were incubated with SIX5 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . SIX5 immunoreactivity is localized to Cytoplasm significantly.

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIX5 antibody
  Rabbit polyclonal anti-SIX5 antibody  
Western blot analysis of extracts from K562 cells, using SIX5 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

SIX5 (Center) antibody
  Rabbit polyclonal SIX5 Antibody (Center)  
SIX5 Antibody (Center) (Cat. #TA324500) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SIX5 antibody detected the SIX5 protein (arrow). Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+2 additional images

SIX5 antibody
Western blot (WB) analysis of Six5 antibody (Cat.-No.: AP01481PU-N) in extracts from K562 cells. Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €370.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SIX5
  Recombinant protein of human SIX homeobox 5 (SIX5)  
SIX5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

SIX5 overexpression lysate
SIX5 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

5 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (3)
  3. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (3)
Target
  1. SIX5 (4)
Property
  1. Center (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (2)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (1)
  3. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (2)
  2. Purified (1)
Host
  1. Rabbit (3)
Images
  1. 1 image (4)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Immunofluorescence (IF) (1)
  3. IHC (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (5)
Service
  • OriGene Technologies GmbH
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  • Germany
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  • fax: +49-5221-34606-11
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