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SLC12A4 antibody

Principal name

SLC12A4 antibody

Alternative names for SLC12A4 antibody

KCC1, Solute carrier family 12 member 4, Erythroid K-Cl cotransporter 1

SwissProt ID

Q28677 (Rabit), Q63632 (Rat), Q9JIS8 (Mouse), Q9UP95 (Human)

Gene ID

6560 (SLC12A4), 29501 (Slc12a4), 20498

Ncbi ID

9606, NP_001139433.1, NP_005063, NP_062102.1, NP_033221.1, NM_009195, NM_019229, NM_005072, NM_001145964, NM_001145961, NM_001145963, NM_001145962

Available reactivities

Hu (Human), Bt (Bat), Bov (Bovine), Can (Canine), Eq (Equine), Hst (Hamster), Mky (Monkey), Ms (Mouse), Prim (Primate), Rb (Rabbit), Rt (Rat), Bear, GP (Guinea Pig), Sh (Sheep)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of SLC12A4 antibody

Potassium chloride cotransporters (KCCs) are members of cation chloride cotransporter gene superfamily (Slc12 family). This family consists of sodium potassium two chloride cotransporter 1 (NKCC1) and 2 (NKCC2), sodium chloride cotransporte (NCC) and KCCs. All of these transporters mediate electroneutral transport of cations coupled with one or two Cl and contribute to regulation of cell volume and homeostasis of intracellular Cl concentration. KCCs extrude one K+ with one Cl from intracellular space to the extracellular space by using the chemical gradient of K+ as the driving force of Cl extrusion. Four KCC isofroms (KCC1, KCC2, KCC3 and KCC4) have been identified in mammalian species . K-Cl cotransporters regulate neurol and glial electrochemical equilibrium potential for Cl, and so can determine the excitatory or inhibitory influences of GABA- and glycine-gated Cl channels. KCC1 is a member of the cation-chloride cotransporter (CCC) superfamily of proteins, as with all CCC proteins, KCC1 is an integral membrane protein with 12 transmembrane domains and both N- and C-termil cytoplasmic domains. KCC1 is ubiquitously expressed. K-Cl cotransport activity is elevated in red blood cells of individuals with sickle cell disease, thalassemia, and diseases associated with other mutant Hb. Human erythrocytes from individuals with the reticulocytotic anemic states of sickle cell disease, Hb SC disease, and β-thalassemia intermedia exhibit uniformly elevated KCC1-like immunoreactivity compared with normal AA erythrocytes .

Western Blot analysis of SLC12A4 expression in transfected 293T cell line by SLC12A4 monoclonal antibody (M01), clone 1H6.Lane 1: SLC12A4 transfected lysate(120.6 KDa).Lane 2: Non-transfected lysate.

H00006560-M01

SLC12A4 polyclonal antibody ( Cat # PAB7375 ) ( 0.3 µg/ml ) staining of human heart lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB7375

AP08770PU-N at 5 µg/ml staining of SLC12A4 in Human Placenta tissue by Immunohistochemistry (FFPE)

AP08770PU-N

AP08770PU-N at 0.3 µg/ml staining of Human Heart lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by Chemiluminescence

AP08770PU-N

Human PANC1; WB Suggested Anti-SLC12A4 Antibody Titration: 0.2-1 ug/ml. Positive Control: PANC1 cell lysate. There is BioGPS gene expression data showing that SLC12A4 is expressed in PANC1.; SLC12A4 antibody - N-terminal region (ARP43895_P050) in Human PANC1 cells using Western Blot

ARP43895_P050

TA303291 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA303291

Predicted cell location: Cytoplasm . Positive control: Human thyroid and colon cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using SLC12A4 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200)

TA323976

Predicted cell location: Cytoplasm . Positive control: Human thyroid and colon cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using SLC12A4 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200)

TA323976

Predicted cell location: Cytoplasm . Positive control: Human brain and thyroid cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using SLC12A4 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200)

TA323977

Predicted cell location: Cytoplasm . Positive control: Human brain and thyroid cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using SLC12A4 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200)

TA323977

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human K562 erythroleukemia cell lysates (lanes 3 and 6): 1-3. Anti-KCC1 antibody, (1:200). 4-6. Anti-KCC1 antibody, preincubated with the control peptide antigen.

TA328867

WB Suggested Anti-SLC12A4 Antibody Titration: 0.2-1 ug/ml; Positive Control: PANC1 cell lysate;There is BioGPS gene expression data showing that SLC12A4 is expressed in PANC1

TA333951

Western blot analysis of SLC12A4 expression in HeLa (A); mouse brain (B); rat brain (C) whole cell lysates.

Immunohistochemical analysis of SLC12A4 staining in rat brain;mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA303291	SLC12A4 antibody
	Goat Polyclonal Antibody against SLC12A4
	Goat		Aff - Purified	Can, Hu, Ms, Rt	WB	0.1 mg / €360.00
						OriGene Technologies, Inc.
	Published recently!
TA323976	SLC12A4 antibody
	Anti-SLC12A4 Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P	0.1 ml / €335.00
						OriGene Technologies, Inc.
+1 additional image
TA323977	SLC12A4 antibody
	Anti-SLC12A4 Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P	0.1 ml / €335.00
						OriGene Technologies, Inc.
+1 additional image
TA328867	SLC12A4 antibody
	Rabbit Polyclonal Anti-KCC1
	Rabbit		Purified	Hu, Ms, Rt	WB	0.2 ml / €585.00
						OriGene Technologies, Inc.
AP08770PU-N	SLC12A4 (997-1007) antibody
	Goat	IgG	Aff - Purified	Bt, Can, Elephant, Hst, Hu, Ms, Rt	E, P, WB	50 µg / €425.00
						OriGene Technologies GmbH
+1 additional image

Human Lenti ORF Particles

Catalog No.
RC228636L2V	Lenti ORF particles, SLC12A4 (mGFP-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €2,290.00
		OriGene Technologies, Inc.

RC228638L2V	Lenti ORF particles, SLC12A4 (mGFP-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 3, 200 uL, >10^7 TU/mL
		200 µl / €2,310.00
		OriGene Technologies, Inc.

RC228637L2V	Lenti ORF particles, SLC12A4 (mGFP-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 4, 200 uL, >10^7 TU/mL
		200 µl / €2,290.00
		OriGene Technologies, Inc.

RC228634L2V	Lenti ORF particles, SLC12A4 (mGFP-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 5, 200 uL, >10^7 TU/mL
		200 µl / €2,260.00
		OriGene Technologies, Inc.

RC228636L1V	Lenti ORF particles, SLC12A4 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €2,070.00
		OriGene Technologies, Inc.

RC228638L1V	Lenti ORF particles, SLC12A4 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 3, 200 uL, >10^7 TU/mL
		200 µl / €2,090.00
		OriGene Technologies, Inc.

RC228637L1V	Lenti ORF particles, SLC12A4 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 4, 200 uL, >10^7 TU/mL
		200 µl / €2,070.00
		OriGene Technologies, Inc.

RC228634L1V	Lenti ORF particles, SLC12A4 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 5, 200 uL, >10^7 TU/mL
		200 µl / €2,040.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR211608L2V	Lenti ORF particles, Slc12a4 (GFP-tagged) - Mouse solute carrier family 12, member 4 (Slc12a4), 200 uL, >10^7 TU/mL
		200 µl / €2,010.00
		OriGene Technologies, Inc.

MR211608L1V	Lenti ORF particles, Slc12a4 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 4 (Slc12a4), 200 uL, >10^7 TU/mL
		200 µl / €1,800.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR201621L2V	Lenti ORF particles, Slc12a4 (GFP-tagged ORF) - Rat solute carrier family 12 (potassium/chloride transporters), member 4 (Slc12a4), 200 uL, >10^7 TU/mL
		200 µl / €2,390.00
		OriGene Technologies, Inc.

RR201621L1V	Lenti ORF particles, Slc12a4 (Myc-DDK-tagged ORF) - Rat solute carrier family 12 (potassium/chloride transporters), member 4 (Slc12a4), 200 uL, >10^7 TU/mL
		200 µl / €2,150.00
		OriGene Technologies, Inc.

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