SLC16A1 antibody
Principal name
SLC16A1 antibody
Alternative names for SLC16A1 antibody
MCT 1, MCT1, Monocarboxylate transporter 1, Solute carrier family 16 member 1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of SLC16A1 antibody
- Kit Component:
- - KN205213G1, SLC16A1 gRNA vector 1 in pCas-Guide vector
- KN205213G2, SLC16A1 gRNA vector 2 in pCas-Guide vector
- KN205213D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Borthakur,A., Am. J. Physiol. Gastrointest. Liver Physiol. 290 (1), G30-G35
Western blot analysis of SLC16A1 polyclonal antibody ( Cat # PAB2599 ) in HL-60 cell line lysates ( 35 µg/lane ) .SLC16A1 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Western blot analysis of SLC16A1 polyclonal antibody ( Cat # PAB2600 ) in CEM cell line lysates ( 35 µg/lane ) .SLC16A1 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Human Jurkat; WB Suggested Anti-SLC16A1 Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate; SLC16A1 antibody - middle region (ARP43841_T100) in Human Jurkat cells using Western Blot
Western blot analysis of anti-SLC16A1 Antibody (C-term) (Cat.#AP12388PU-N) in HL60 cell line lysates (35ug/lane). SLC16A1(arrow) was detected using the purified Pab (1:60dilution).
Western blot analysis of anti-SLC16A1 Antibody (Center) (Cat.#AP12389PU-N) in CEM cell line lysates (35ug/lane). SLC16A1 (arrow) was detected using the purified Pab (1:60dilution).
Immunofluorescence of purified MaxPab antibody to SLC16A1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of SLC16A1 expression in transfected 293T cell line (H00006566-T02) by SLC16A1 MaxPab polyclonal antibody.Lane 1: SLC16A1 transfected lysate(55 KDa).Lane 2: Non-transfected lysate.
Human Bronchial Epithelial Tissue; Rabbit Anti-SLC16A1 Antibody . Catalog Number: ARP43842_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue . Observed Staining: Membrane and cytoplasmic . Primary Antibody Concentration: 1:100 . Other Working Concentrations: 1/600 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-SLC16A1 Antibody (ARP43842_P050) ) in Human Bronchial Epithelial Tissue using Immunohistochemistry
Human HT1080; WB Suggested Anti-SLC16A1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HT1080 cell lysate. SLC16A1 is strongly supported by BioGPS gene expression data to be expressed in Human HT1080 cells.; SLC16A1 antibody - middle region (ARP43842_P050) in Human HT1080 cells using Western Blot
Predicted band size: 54 kDa . Positive control: Human seminoma tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)
Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human liver cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using SLC16A1 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human liver and ovarian cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using SLC16A1 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human liver and ovarian cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using SLC16A1 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
WB Suggested Anti-SLC16A1 Antibody Titration: 2.5 ug/ml; Positive Control: Jurkat cell lysate
WB Suggested Anti-SLC16A1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HT1080 cell lysate;SLC16A1 is strongly supported by BioGPS gene expression data to be expressed in Human HT1080 cells
Rabbit Anti-SLC16A1 Antibody; Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue; Observed Staining: Membrane and cytoplasmic; Primary Antibody Concentration: 1:100; Other Working Concentrations: 1/600; Secondary Antibody: Donkey a
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305213 | SLC16A1 |
||||
| Recombinant protein of human solute carrier family 16, member 1 (monocarboxylic acid transporter 1) (SLC16A1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
