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SLIT2 antibody

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Principal name

SLIT2 antibody

Alternative names for SLIT2 antibody

SLIT-2, SLIL3, Slit homolog 2 protein

SwissProt ID

O94813 (Human), Q9R1B9 (Mouse), Q9WVC1 (Rat)

Gene ID

9353 (SLIT2), 20563 (Slit2)

Ncbi ID

9606, NP_848919, NP_004778, O94813, NP_004778.1, NP_848919.3, NM_178804, NM_004787

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Rt (Rat), Can (Canine), Xen (Xenopus)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)

Background of SLIT2 antibody

SLIT-2 is thought to act as a molecular guidance cue in cellular migration, and its function appears to be mediated by interaction with roundabout homologue receptors. SLIT-1 and SLIT-2 seem to be essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development SLIT-2 may play role in guiding commissural axons once they reach the floor plate by modulating the response to netrin. In vitro, SLIT-2 silences the attractive effect of NTN1 but not its growthstimulatory effect and silencing requires the formation of a ROBO1-DCC complex. In vitro, only commissural axons that crossed the midline responded to SLIT-2. In the developing visual system this protein functions as a repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. SLIT-2 is a secreted protein predominantly expressed in fetal lung and kidney, and adult spinal cord. Weak expression is observed in adult adrenal gland, thyroid, trachea and other tissues examined. Multiple isoforms have been reported for this product.

PAB11259 - SLIT2
Western blot using SLIT2 polyclonal antibody ( Cat # PAB11259 ) shows detection of a band at ~165 kDa ( lane 1 ) corresponding to SLIT2 present in a chicken spinal cord whole cell lysate ( arrowhead ).Approximately 30 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1 : 1,350.Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature ( 800 nm channel, green ).Molecular weight estimation was made by comparison to prestained MW markers in lane M ( 700 nm channel, red ).IRDyeTM 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
AP22401PU-N - SLIT2
AP22401PU-N SLIT2 antibody staining of Human prostate adenocarcinoma.
AM21041PU-N - SLIT2
Human Prostate Carcinoma stained with SLIT2 Antibody Cat.-No AM21041PU (Clone SP122).
AM21041PU-N - SLIT2
Flow Cytometric analysis of Rabbit anti-SLIT2 Antibody Cat.-No AM21041PU (Clone SP122) in SH-SY5Y (green) compare to negative control of Rabbit IgG (blue).
TP320894 - SLIT2
TP723415 - SLIT2
TA311312 - SLIT2
TA311312 (4ug/ml) staining of paraffin embedded Human Spinal Cord. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
TA319177 - SLIT2
WB using Anti-SLIT-2 antibody shows detection of a band at ~165 kDa (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). The primary antibody diluted to 1:1,350. Reaction occurred overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
TA354077 - SLIT2
Human Prostate Adenocarcinoma stained with anti-SLIT2 antibody.
TA354077 - SLIT2
Flow cytometric analysis of rabbit anti-SLIT2 (SP122) antibody in SH-SY5Y (green) compare to negative control of rabbit IgG (blue).
TA354077 - SLIT2
Human Prostate Adenocarcinoma stained with anti-SLIT2 antibody.
TA354077 - SLIT2
Human Placenta stained with anti-SLIT2 antibody.
TA354077 - SLIT2
Human Breast stained with anti-SLIT2 antibody.
TA354077 - SLIT2
Human Colon stained with anti-SLIT2 antibody.
TA354077 - SLIT2
Human Colon Adenocarcinoma stained with anti-SLIT2 antibody.
TA354077 - SLIT2
Human Kidney stained with anti-SLIT2 antibody.
TA354077 - SLIT2
Human Melanoma stained with anti-SLIT2 antibody.
TA354077 - SLIT2
Human Prostate Adenocarcinoma stained with anti-SLIT2 antibody.
TA354076 - SLIT2
Human Prostate Carcinoma stained with anti-SLIT2 antibody.
TA351720 - SLIT2
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Mouse bladder tissue, Primary antibody: (SLIT2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
TA351720 - SLIT2
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (SLIT2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
TA351720 - SLIT2
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (SLIT2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)

6 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLIT2 antibody
  Goat Anti-SLIT2 Antibody  
TA311312 (4ug/ml) staining of paraffin embedded Human Spinal Cord. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. Goat Aff - Purified Can, Hu, Ms, Rt E, P
0.1 mg / €360.00
  OriGene Technologies, Inc.

SLIT2 (484-500) antibody
  Rabbit polyclonal anti-SLIT-2 antibody  
WB using Anti-SLIT-2 antibody shows detection of a band at ~165 kDa (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). The primary antibody diluted to 1:1,350. Reaction occurred overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Rabbit IgG Aff - Purified Bov, Chk, Hu, Ms, Rt, Tetraodon nigroviridis, Xen E, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.

SLIT2 antibody
  Rabbit Polyclonal Anti-SLIT2 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Mouse bladder tissue, Primary antibody: (SLIT2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+2 additional images

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SLIT2
  Recombinant protein of human slit homolog 2 (Drosophila) (SLIT2)  
SLIT2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.

SLIT2
  Purified recombinant protein of Human slit homolog 2 (Drosophila) (SLIT2).  
SLIT2 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
25 µg / €230.00
  OriGene Technologies, Inc.
 

SLIT2
Cultured chick dorsal root ganglion neurons were grown in the presence of recombinant human NGF-b with (A) or without (B) recombinant mouse Slit2. The presence of the Slit2 protein signifi cantly enhanced neurite outgrowth. Mouse Purified > 97 % CHO cells
50 µg / €400.00
  Neuromics Antibodies

6 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (3)
  2. Protein | Growth Factor (3)
Clonality
  1. Polyclonal (3)
Target
  1. SLIT2 (6)
Property
  1. 484-500 (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (3)
  3. Rat (Rt) (3)
  4. Bovine (Bov) (1)
  5. Canine (Can) (1)
  6. Chicken (Chk) (1)
  7. Tetraodon nigroviridis (1)
  8. Xenopus (Xen) (1)
Applications
  1. Enzyme Immunoassay (E) (3)
  2. Paraffin Sections (P) (2)
  3. Western blot / Immunoblot (WB) (2)
Presentation
  1. Aff - Purified (2)
  2. Purified (2)
Host
  1. Rabbit (2)
  2. Goat (1)
Images
  1. 1 image (6)
  2. 3+ images (1)
Images: Applications
  1. IHC (2)
  2. Western blot / Immunoblot (WB) (2)
Species (Protein)
  1. Human (2)
  2. Mouse (1)
Source
  1. HEK293 cells (2)
  2. CHO cells (1)
Manufacturer
  1. OriGene Technologies (5)
  2. Neuromics Antibodies (1)
Service
  • OriGene Technologies GmbH
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  • Germany
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  • free call: 0800-227 47 46 (Germany only)
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