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SMAD6 antibody

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Principal name

SMAD6 antibody

Alternative names for SMAD6 antibody

SMAD family member 6, SMAD 6, SMAD-6, MADH6, MAD homolog 6, Mothers against decapentaplegic homolog 6, Mothers against DPP homolog 6

SwissProt ID

O35182 (Mouse), O43541 (Human), Q9W734 (Chick)

Gene ID

4091 (SMAD6), 17130 (Smad6)

Ncbi ID

9606, NP_005576, NP_001136333.1, NP_001136333, NP_032568.3, NM_008542, NM_005585

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Prim (Primate), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunoprecipitation (IP), Frozen Sections (C)

Background of SMAD6 antibody

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the sigling pathways of the serine/threonine kise receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alterte mes for one another in the literature. Human SMAD1 is a 465 amino acid protein; GenBank Accession No. AAP36050.1. Human SMAD2 is a 467 amino acid protein; GenBank Accession No. AAC51918.1 Human SMAD3 is a 425 amino acid protein; GenBank Accession No. NP_005893.1 Human SMAD4 is a 552 amino acid protein GenBank Accession No. NP_005350.1. Human SMAD5 is a 465 amino acid protein; GenBank Accession No. AAC50791.1. Human SMAD6 is a 496 amino acid protein; GenBank Accession No. AAH12986.1 Human SMAD7 is a 426 amino acid protein; GenBank Accession No. AAB81354.1 Mouse SMAD8 is a 430 amino acid protein; GenBank Accession No. AAN85445.1 Human SMAD9 is a 430 amino acid protein; GenBank Accession No. NP_005896.1

General readings

Tsunobuchi,H., et al., (2004) Biophys. Res. Commun. 316(3),712-719

H00004091-M01 - SMAD6
Immunoperoxidase of monoclonal antibody to SMAD6 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]
H00004091-M02 - SMAD6
Immunoperoxidase of monoclonal antibody to SMAD6 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 ug/ml]
H00004091-M03 - SMAD6
Immunoperoxidase of monoclonal antibody to SMAD6 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]
H00004091-M03 - SMAD6
Detection limit for recombinant GST tagged SMAD6 is approximately 3ng/ml as a capture antibody.
H00004091-M06 - SMAD6
SMAD6 monoclonal antibody (M06), clone 2A8. Western Blot analysis of SMAD6 expression in human lung cancer.
H00004091-M07 - SMAD6
SMAD6 monoclonal antibody (M07), clone 4F4. Western Blot analysis of SMAD6 expression in MCF-7 ( Cat # L046V1 ).
H00004091-M07 - SMAD6
Detection limit for recombinant GST tagged SMAD6 is approximately 0.03ng/ml as a capture antibody.
H00004091-M08 - SMAD6
Detection limit for recombinant GST tagged SMAD6 is approximately 3ng/ml as a capture antibody.
H00004091-M09 - SMAD6
Immunoperoxidase of monoclonal antibody to SMAD6 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]
H00004091-M09 - SMAD6
Detection limit for recombinant GST tagged SMAD6 is approximately 3ng/ml as a capture antibody.
P100717_P050 - SMAD6
Human Jurkat; WB Suggested Anti-SMAD6 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that SMAD6 is expressed in Jurkat.; SMAD6 antibody - N-terminal region (P100717_P050) in Human Jurkat cells using Western Blot
ARP32690_T100 - SMAD6
Human Heart; SMAD6 antibody - N-terminal region (ARP32690_T100) in Human Heart cells using Immunohistochemistry
ARP32690_T100 - SMAD6
Human Lung; SMAD6 antibody - N-terminal region (ARP32690_T100) in Human Lung cells using Immunohistochemistry
ARP32690_T100 - SMAD6
Human Jurkat; WB Suggested Anti-SMAD6 Antibody Titration: 0.5-1.0ug/ml. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that SMAD6 is expressed in Jurkat.; SMAD6 antibody - N-terminal region (ARP32690_T100) in Human Jurkat cells using Western Blot
PAB0368 - SMAD6
Western blot analysis of SMAD6 in 30 ug of Jurkat cell lysate using SMAD6 polyclonal antibody ( Cat # PAB0368 ) at 2 µg/ml.
H00004091-B01 - SMAD6
Western Blot analysis of SMAD6 expression in transfected 293T cell line (H00004091-T01) by SMAD6 MaxPab polyclonal antibody.Lane 1: SMAD6 transfected lysate(54.56 KDa).Lane 2: Non-transfected lysate.
H00004091-B01 - SMAD6
FACS analysis of negative control 293 cells (Black) and SMAD6 expressing 293 cells (Green) using SMAD6 purified MaxPab mouse polyclonal antibody.
H00004091-B01P - SMAD6
Western Blot analysis of SMAD6 expression in transfected 293T cell line (H00004091-T01) by SMAD6 MaxPab polyclonal antibody.Lane 1: SMAD6 transfected lysate(54.56 KDa).Lane 2: Non-transfected lysate.
H00004091-B01P - SMAD6
FACS analysis of negative control 293 cells (Black) and SMAD6 expressing 293 cells (Green) using SMAD6 purified MaxPab mouse polyclonal antibody.
H00004091-D01P - SMAD6
Western Blot analysis of SMAD6 expression in transfected 293T cell line (H00004091-T02) by SMAD6 MaxPab polyclonal antibody.Lane 1: SMAD6 transfected lysate(53.50 KDa).Lane 2: Non-transfected lysate.
AP08466PU-N - SMAD6
Testis: Formalin-Fixed Paraffin-Embedded (FFPE)
AP20668PU-N - SMAD6
Immunohistochemistry (IHC) analyzes of Smad6 antibody (Cat.-No.: AP20668PU-N) in paraffin-embedded human lung carcinoma tissue.
AM20911PU-N - SMAD6
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20912PU-N - SMAD6
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20913PU-N - SMAD6
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20913PU-N - SMAD6
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20913PU-N - SMAD6
Detection limit for recombinant GST tagged SMAD6 is approximately 3 ng/ml as a capture antibody.
AM20914PU-N - SMAD6
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20914PU-N - SMAD6
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20914PU-N - SMAD6
Detection limit for recombinant GST tagged SMAD6 is approximately 3 ng/ml as a capture antibody.
AM06507SU-N - SMAD6
Western blot analysis using SMAD6 antibody Cat.-No AM06507SU-N against A431 (1), A431 (2), Hela (3) and Jurkat (4) cell lysate.
CPA1711-100ul - SMAD6
Immunohistochemical analysis of SMAD6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA1711-100ul - SMAD6
Western blot analysis of SMAD6 expression in A204 (A); LN18 (B); L929 (C) whole cell lysates.
TA324805 - SMAD6
SMAD6 Antibody (Center) (Cat. #TA324805) western blot analysis in HepG2,HT29 cell line lysates (35ug/lane).This demonstrates the SMAD6 antibody detected the SMAD6 protein (arrow).
TA324805 - SMAD6
SMAD6 Antibody (Center) (Cat. #TA324805) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA329745 - SMAD6
Host: Rabbit; Target Name: SMAD6; Sample Tissue: Thymus Tumor lysates; Antibody Dilution: 1.0ug/ml
TA330050 - SMAD6
WB Suggested Anti-SMAD6 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysateThere is BioGPS gene expression data showing that SMAD6 is expressed in Jurkat
TA331110 - SMAD6
Host: Rabbit; Target Name: Smad6; Sample Tissue: Mouse Kidney lysates; Antibody Dilution: 1.0ug/ml
TA331111 - SMAD6
WB Suggested Anti-SMAD6 Antibody Titration: 0.5-1.0ug/ml; Positive Control: Jurkat cell lysateThere is BioGPS gene expression data showing that SMAD6 is expressed in Jurkat
TA331111 - SMAD6
Human Lung
TA331111 - SMAD6
Human Heart
TA336359 - SMAD6
Western Blot: SMAD6 Antibody [TA336359] - analysis using SMAD6 antibody. Lysate from Jurkat cells probed with SMAD6 antibody at 2 ug/ml.

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMAD6 antibody
  Rabbit polyclonal SMAD6 Antibody (Center)  
SMAD6 Antibody (Center) (Cat. #TA324805) western blot analysis in HepG2,HT29 cell line lysates (35ug/lane).This demonstrates the SMAD6 antibody detected the SMAD6 protein (arrow). Rabbit IgG Purified Hu, Ms F, WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

SMAD6 antibody
  Rabbit Polyclonal SMAD6 Antibody  
Western Blot: SMAD6 Antibody [TA336359] - analysis using SMAD6 antibody. Lysate from Jurkat cells probed with SMAD6 antibody at 2 ug/ml. Rabbit Purified Hu, Ms, Prim, Rt, Sh C, WB
0.1 mg / €450.00
  OriGene Technologies, Inc.

SMAD6 antibody
Western blot analysis using SMAD6 antibody Cat.-No AM06507SU-N against A431 (1), A431 (2), Hela (3) and Jurkat (4) cell lysate. Mouse IgG1 5H3 Ascites Hu E, WB
0.1 ml / €380.00
  OriGene Technologies GmbH

SMAD6 antibody
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 4C8 Hu E, P, WB
50 µg / €475.00
  OriGene Technologies GmbH

SMAD6 (285-385) antibody
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 4F3 Purified Hu E, P, WB
50 µg / €475.00
  OriGene Technologies GmbH

SMAD6 (285-385) antibody
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 2A6 Purified Hu E, P, WB
50 µg / €475.00
  OriGene Technologies GmbH
+2 additional images

SMAD6 antibody
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1G2 Hu E, P, WB
50 µg / €475.00
  OriGene Technologies GmbH
+2 additional images

SMAD6 (85-99, 372-388) antibody
Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Por P, WB
50 µg / €425.00
  OriGene Technologies GmbH

SMAD6 antibody
Immunohistochemistry (IHC) analyzes of Smad6 antibody (Cat.-No.: AP20668PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €370.00
  OriGene Technologies GmbH

SMAD6 (Isoform D) antibody
SMAD6 Rabbit Serum Hu C, E, WB
0.2 ml / €430.00
  OriGene Technologies GmbH

10 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (10)
Clonality
  1. Monoclonal (5)
  2. Polyclonal (5)
Target
  1. SMAD6 (10)
Property
  1. 285-385 (2)
  2. 85-99, 372-388 (1)
  3. Isoform D (1)
Reactivity
  1. Human (Hu) (10)
  2. Mouse (Ms) (3)
  3. Rat (Rt) (2)
  4. Porcine (Por) (1)
  5. Primate (Prim) (1)
  6. Sheep (Sh) (1)
Applications
  1. Western blot / Immunoblot (WB) (9)
  2. Enzyme Immunoassay (E) (6)
  3. Paraffin Sections (P) (6)
  4. Frozen sections (C) (2)
  5. Flow cytometry (F) (1)
Presentation
  1. Purified (4)
  2. Aff - Purified (2)
  3. Ascites (1)
  4. Serum (1)
Host
  1. Mouse (5)
  2. Rabbit (5)
Images
  1. 1 image (9)
  2. 3+ images (2)
Images: Applications
  1. Paraffin Sections (P) (6)
  2. Western blot / Immunoblot (WB) (3)
  3. ELISA (capture) (E(capture)) (2)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
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  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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