SMAD9 antibody
Principal name
SMAD9 antibody
Alternative names for SMAD9 antibody
SMAD family member 9, SMAD-9, SMAD 9, MADH9, MAD homolog 9, Mothers against decapentaplegic homolog 9, Mothers against DPP homolog 9
Ncbi ID
NP_001120689.1, 9606, NP_005896, NP_001120689, NP_062356.3, NM_019483, NM_005905, NM_001127217, NM_138872
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of SMAD9 antibody
- Kit Component:
- - KN204143G1, SMAD9 gRNA vector 1 in pCas-Guide vector
- KN204143G2, SMAD9 gRNA vector 2 in pCas-Guide vector
- KN204143D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Shi Y, Massague J.: Mechanisms of TGF-beta signaling from cell membrane to the nucleus. Cell. 2003 Jun 13;113(6):685-700. Review. PMID: 12809600
Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Antibody (0.3 µg/ml) staining of Human Skin lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot analysis of SMAD9 expression in transfected 293T cell line (H00004093-T04) by SMAD9 MaxPab polyclonal antibody.Lane 1: SMAD9 transfected lysate(25.30 KDa).Lane 2: Non-transfected lysate.
SMAD9 polyclonal antibody ( Cat # PAB7003 ) ( 0.3 µg/ml ) staining of human skin lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescen
Sample (30 ug of whole cell lysate). A: NT2D1. 10% SDS PAGE. TA308344 diluted at 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMAD9(TA308344) antibody at 1:500 dilution.
SMAD9 Antibody (Center) (Cat. #TA324579) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMAD9 antibody detected the SMAD9 protein (arrow).
IF image of MCF-7 cell stained with SMAD9 Antibody (Center)(Cat#TA324579). MCF-7 cells were incubated with SMAD9 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.
TA303098 (0.3µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304143 | SMAD9 (transcript variant b) |
||||
| Recombinant protein of human SMAD family member 9 (SMAD9), transcript variant b | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||