SMG7 antibody
Principal name
SMG7 antibody
Alternative names for SMG7 antibody
SMG-7, SMG-7 homolog, C1orf16, EST1C, EST1-like protein C, KIAA0250
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)
Background of SMG7 antibody
- Kit Component:
- - KN316313G1, Smg7 gRNA vector 1 in pCas-Guide vector
- KN316313G2, Smg7 gRNA vector 2 in pCas-Guide vector
- KN316313D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunohistochemical analysis of SMG7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of SMG7 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of SMG7 expression in Jurkat (A); A431 (B); HeLa (C) whole cell lysates.
Western blot analysis of extracts from HeLa cells, Jurkat cells, 293 cells and HUVEC cells, using SMG7 antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using SMG7 antibody.The picture on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314983 | SMG7 antibody |
||||||
| Rabbit polyclonal anti-SMG7 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY433658 | SMG7 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC220759L2V | Lenti ORF particles, SMG7 (mGFP-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,330.00
|
|
| OriGene Technologies, Inc. | ||
| RC230659L2V | Lenti ORF particles, SMG7 (mGFP-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 5, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,410.00
|
|
| OriGene Technologies, Inc. | ||
| RC220759L1V | Lenti ORF particles, SMG7 (Myc-DDK-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,100.00
|
|
| OriGene Technologies, Inc. | ||
| RC230659L1V | Lenti ORF particles, SMG7 (Myc-DDK-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 5, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,180.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211685L2V | Lenti ORF particles, Smg7 (GFP-tagged) - Mouse Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,070.00
|
|
| OriGene Technologies, Inc. | ||
| MR211685L1V | Lenti ORF particles, Smg7 (Myc-DDK-tagged) - Mouse Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,870.00
|
|
| OriGene Technologies, Inc. | ||
