SMO antibody
Principal name
SMO antibody
Alternative names for SMO antibody
SMOH, Smoothened homolog, Gx protein
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of SMO antibody
Overexpression of either Wnt-1 or the GLI proteins results in cancer; however, the molecular basis for this transformation was poorly understood. The Wnt-1 and GLI proteins have now been placed in a signaling cascade downstream of the mammalian homologs of the Drosophila hedgehog and patched proteins. The Drosophila segment polarity gene hedgehog (hh) encodes a 19-25 kDa secreted protein that appears to function in embryonic and imaginal disc patterning. The ptc gene, also identified as a Drosophila segment polarity gene, encodes the transmembrane protein patched, the expression of which is precisely regulated during embryonic development. Hedgehog has been shown to enhance the expression of the Wnt family of proteins through a signaling cascade involving the GLI transcription factors, while patched functions as a repessor opposing hedgehog’s effects. Smoothened (Smo), a seven transmembrane receptor, is complexed with patched in many tissues and is believed to be an essential component in the Hh signaling pathway.
General readings
Taipale J, Cooper MK, Maiti T, Beachy PA.
Patched acts catalytically to suppress the activity of Smoothened.
Nature. 2002 Aug 22;418(6900):892-7. Erratum in: Nature 2002 Nov
28;420(6914):445.
Detection limit for recombinant GST tagged SMO is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SMO is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SMO is approximately 0.1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SMO is approximately 0.1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SMO is approximately 0.3ng/ml as a capture antibody.
Western blot using SMO antibody on human brain lysate. Lysate loaded at 15 µg/lane. Antibody used at 10 µg/ml (1) and 5 µg/ml (2) dilution. Secondary antibody, mouse anti-rabbit-HRP, used at 1:150K dilution.
Western blot using SMO antibody (AP05293SU-N) on human brain lysate. Lysate loaded at 15 µg/lane. Antibody used at 10 µg/ml (1) and 5 µg/ml (2) dilution. Secondary antibody, mouse anti-rabbit-HRP, used at 1:150K dilution.
Immunohistochemical analysis of human SMO in formalin-fixed paraffin-embedded human brain tissue using an isotype control ( top ) and SMO polyclonal antibody ( Cat # PAB0305 ) ( bottom ) at 10 µg/ml.
Western blot analysis of human SMO in human heart lysate in the 1 ) absence and 2 ) presence of immunizing peptide. Using SMO polyclonal antibody ( Cat # PAB0305 ) at 0.1 µg/ml.
SMO polyclonal antibody ( Cat # PAB6915 ) ( 0.3 µg/ml ) staining of human bone marrow lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
SMO polyclonal antibody ( Cat # PAB7120 ) ( 0.3 µg/ml ) staining of human bone marrow lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
SMO monoclonal antibody (M11), clone 1D9 Western Blot analysis of SMO expression in A-431 ( Cat # L015V1 ).
Western blot (WB) analysis of Smo antibody (Cat.-No.: AP01508PU-N) in extracts from HUVEC cells.
Western blot using SMO polyclonal antibody ( Cat # PAB10404 ) on human brain lysate. Lysate loaded at 15 µg/lane. Antibody used at 10 µg/ml(1) and 5 µg/ml (2) dilution.
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts from HuvEc cells, using SMO Antibody. The lane on the right is treated with the synthesized peptide.
SMO Antibody (Center) (Cat. #AP53947PU-N) western blot analysis in HepG2 cell line lysates (35µg/lane).This demonstrates the SMO antibody detected the SMO protein (arrow).
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection limit for recombinant GST tagged SMO is 0.1 ng/ml as a capture antibody.
Immunohistochemical analysis of Smoothened staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of Smoothened expression in HeLa (A) whole cell lysates.
A) TA303075 and B) TA303149 (0.3µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
A) TA303075 and B) TA303149 (0.3µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts from HuvEc cells, using SMO antibody.The lane on the right is treated with the synthesized peptide.
Anti-SMO antibody IHC of human placenta, cytotrophoblast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Predicted cell location: Cytoplasm. Positive control: Human brain and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using SMO antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human brain and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using SMO antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western Blot: Smoothened Antibody [TA337000] - Analysis in human heart lysate in the 1) absence and 2) presence of immunizing peptide using SMO antibody at 0.1 ug/ml.
Immunohistochemistry-Paraffin: Smoothened Antibody [TA337000] - Immunohistochemical analysis of Smoothened in formalin-fixed paraffin-embedded human brain tissue using an isotype control antibody (top) and Smoothened antibody (bottom) at 10 ug/ml.
Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Testis: Formalin-Fixed Paraffin-Embedded (FFPE)
Anti-SMO / Smoothened antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SMO / Smoothened antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SMO / Smoothened antibody IHC of human Skin, Basal Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA312846 | SMO antibody |
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| Rabbit polyclonal anti-SMO antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA322573 | SMO antibody |
||||||
| Anti-SMO Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA341345 | SMO (N-term, extracell. dom.) antibody |
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| Rabbit Polyclonal Anti-SMO Antibody (N-Terminal) | |||||||
 |
Rabbit | Aff - Purified | Bear, Bov, Bt, Can, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rb, Rt, Tr | P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341357 | SMO (1st Extracell. Dom.) antibody |
||||||
| Rabbit Polyclonal Anti-SMO Antibody (1st Extracellular Domain) | |||||||
|
Rabbit | Aff - Purified | Bear, Bov, Bt, Can, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Por, Rb, Rt, Xen | ICC/IF, P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| AP01508PU-N | SMO antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| AP05293PU-N | SMO antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu | WB |
0.1 mg /
€400.00
|
|
| OriGene Technologies GmbH | |||||||
| AP05293SU-N | SMO antibody |
||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€400.00
|
|
| OriGene Technologies GmbH | |||||||
| AP23648PU-N | SMO (N-term) antibody |
||||||
 |
Rabbit | IgG | Hu, Ms, Rt | E, P, WB |
50 µl /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AP53947PU-N | SMO (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY417163 | SMO overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR210260L2V | Lenti ORF particles, Smo (GFP-tagged ORF) - Rat smoothened homolog (Drosophila) (Smo), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,600.00
|
|
| OriGene Technologies, Inc. | ||
| RR210260L1V | Lenti ORF particles, Smo (Myc-DDK-tagged ORF) - Rat smoothened homolog (Drosophila) (Smo), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,440.00
|
|
| OriGene Technologies, Inc. | ||