SMYD1 antibody
Principal name
SMYD1 antibody
Alternative names for SMYD1 antibody
SET and MYND domain-containing protein 1
Ncbi ID
NP_033892, NP_938015, ACE87349.1, NP_938015.1, NP_001153599.1, NM_001160127, NM_009762
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB)
Background of SMYD1 antibody
- Kit Component:
- - KN316362G1, Smyd1 gRNA vector 1 in pCas-Guide vector
- KN316362G2, Smyd1 gRNA vector 2 in pCas-Guide vector
- KN316362D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Phan,D., et al., (2005) Development 132 (11), 2669-2678
Mouse NIH-3T3; WB Suggested Anti-SMYD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: NIH/3T3 cell lysate; SMYD1 antibody - N-terminal region (ARP37022_P050) in Mouse NIH-3T3 cells using Western Blot
Human ACHN; WB Suggested Anti-SMYD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: ACHN cell lysate; SMYD1 antibody - N-terminal region (ARP50861_P050) in Human ACHN cells using Western Blot
Human HeLa; WB Suggested Anti-SMYD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate; SMYD1 antibody - middle region (ARP50862_P050) in Human HeLa cells using Western Blot
SMYD1 Antibody (C-term) (Cat. #AP53954PU-N) western blot analysis in Hela cell line lysates (35µg/lane).This demonstrates the SMYD1 antibody detected the SMYD1 protein (arrow).
SMYD1 Antibody (C-term) (Cat. #AP53954PU-N) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-SMYD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: ACHN cell lysate
WB Suggested Anti-SMYD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Hela cell lysate
WB Suggested Anti-SMYD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: NIH/3T3 cell lysate
Western blot detection of SMYD1 in rat skeletal muscle lysates and using SMYD1 mouse mAb (1:100 diluted). Predicted band size: 56KDa.Observed band size: 56KDa.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA347053 | SMYD1 antibody |
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| Mouse Monoclonal SMYD1 Antibody | |||||||
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Mouse | IgG2b | 2E11-B6-C7 | Purified | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP53954PU-N | SMYD1 (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP321269 | SMYD1 |
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| Recombinant protein of human SET and MYND domain containing 1 (SMYD1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207844L2V | Lenti ORF particles, Smyd1 (GFP-tagged) - Mouse SET and MYND domain containing 1 (Smyd1), transcript variant 1, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR207844L1V | Lenti ORF particles, Smyd1 (Myc-DDK-tagged) - Mouse SET and MYND domain containing 1 (Smyd1), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||