SMYD3 antibody
Principal name
SMYD3 antibody
Alternative names for SMYD3 antibody
ZMYND1, ZNFN3A1, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1
Ncbi ID
9606, NP_073580, NP_073580.1, NM_022743, NP_001161212.1, NP_081464.1, NM_027188, NM_001167740
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ChIP assay (ChIP), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of SMYD3 antibody
- Kit Component:
- - KN316364G1, Smyd3 gRNA vector 1 in pCas-Guide vector
- KN316364G2, Smyd3 gRNA vector 2 in pCas-Guide vector
- KN316364D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Silva,F.P., (2008) Oncogene 27 (19), 2686-2692
Human MCF-7; WB Suggested Anti-SMYD3 Antibody Titration: 0.2-1 ug/ml. Positive Control: MCF7 cell lysate; SMYD3 antibody - C-terminal region (ARP50667_P050) in Human MCF-7 cells using Western Blot
Human Stomach; WB Suggested Anti-SMYD3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Stomach; SMYD3 antibody - N-terminal region (ARP50666_P050) in Human Stomach cells using Western Blot
HCT116; Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.; SMYD3 antibody - N-terminal region (ARP50666_P050) in HCT116 using CHIP
Western blot analysis of SMYD3 antibody (N-term) (AP17751PU-N) in CEM cell line lysates (35ug/lane). SMYD3 (arrow) was detected using the purified Pab.
WB Suggested Anti-SMYD3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Stomach
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
WB Suggested Anti-SMYD3 Antibody Titration: 0.2-1 ug/ml; Positive Control: MCF7 cell lysate
Gel: 8%SDS-PAGE,Lysate: 40 ug,Lane 1-3: 293T cells, Hela cells, 231 cells,Primary antibody: (SMYD3 Antibody) at dilution 1/400 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 5 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (SMYD3 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA350428 | SMYD3 antibody |
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| Rabbit Polyclonal Anti-SMYD3 rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| AP17751PU-N | SMYD3 (N-term) antibody |
||||||
 |
Rabbit | Hu | WB |
0.4 ml /
€415.00
|
|||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305933 | SMYD3 |
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| Recombinant protein of human SET and MYND domain containing 3 (SMYD3) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402935 | SMYD3 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206824L2V | Lenti ORF particles, Smyd3 (GFP-tagged) - Mouse SET and MYND domain containing 3 (Smyd3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR206824L1V | Lenti ORF particles, Smyd3 (Myc-DDK-tagged) - Mouse SET and MYND domain containing 3 (Smyd3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
