SMYD4 antibody
Principal name
SMYD4 antibody
Alternative names for SMYD4 antibody
KIAA1936, SET and MYND domain-containing protein 4
Ncbi ID
NP_443160, NM_052928, NP_443160.2, NP_001096081.1, NM_001102611
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of SMYD4 antibody
- Kit Component:
- - KN316365G1, Smyd4 gRNA vector 1 in pCas-Guide vector
- KN316365G2, Smyd4 gRNA vector 2 in pCas-Guide vector
- KN316365D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Human 721_B; WB Suggested Anti-SMYD4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 721_B cell lysate; SMYD4 antibody - N-terminal region (ARP50738_P050) in Human 721_B cells using Western Blot
SMYD4 Antibody (C-term) (Cat. #AP53955PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SMYD4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
SMYD4 Antibody (C-term) (Cat. #AP53955PU-N) western blot analysis in MDA-MB435 cell line lysates (35µg/lane).This demonstrates the SMYD4 antibody detected the SMYD4 protein (arrow).
SMYD4 Antibody (C-term) (Cat. #AP53955PU-N) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-SMYD4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 721_B cell lysate
Western blot detection of SMYD4 in Hela,MCF7 and A431 cell lysates using SMYD4 mouse mAb (1:200 diluted).Predicted band size:89KDa.Observed band size:89KDa.
Western blot detection of SMYD4 in 40ug Hela,40ug MCF7 and 40ug MDA-MB-468 cell lysates using SMYD4 mouse mAb (1:100 diluted).Predicted band size:89KDa.Observed band size:89KDa.
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (SMYD4 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (SMYD4 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA347055 | SMYD4 antibody |
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| Mouse Monoclonal SMYD4 Antibody | |||||||
 |
Mouse | IgG1 | 7C8-B11-F10 | Purified | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA350429 | SMYD4 antibody |
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| Rabbit Polyclonal Anti-SMYD4 rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP53955PU-N | SMYD4 (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu, P | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307305 | SMYD4 |
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| Recombinant protein of human SET and MYND domain containing 4 (SMYD4) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY409386 | SMYD4 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR210723L2V | Lenti ORF particles, Smyd4 (GFP-tagged) - Mouse SET and MYND domain containing 4 (Smyd4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,600.00
|
|
| OriGene Technologies, Inc. | ||
| MR210723L1V | Lenti ORF particles, Smyd4 (Myc-DDK-tagged) - Mouse SET and MYND domain containing 4 (Smyd4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,440.00
|
|
| OriGene Technologies, Inc. | ||