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SNAP23 antibody

Principal name

SNAP23 antibody

Alternative names for SNAP23 antibody

SNAP-23, Synaptosomal-associated protein 23, Vesicle-membrane fusion protein SNAP-23

SwissProt ID

O00161 (Human), O09044 (Mouse), O70377 (Rat)

Gene ID

8773 (SNAP23), 20619 (Snap23), 64630

Ncbi ID

9606, NP_003816.2, NP_003816, NP_001171263.1, NM_001177793, NM_009222, NM_001177792, NM_022689, NM_003825, NM_130798

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Ye (Yeast)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of SNAP23 antibody

SNAP23(Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans is encoded by the SNAP23 gene. The SNAP23 gene has 8 exons, with the initiation codon located in exon 2. The SNAP23 gene is mapped on 15q15.1-q15.2. The SNAP23 cDNA encodes a 211-amino acid polypeptide with a predicted mass of 23 kD. Its amino acid sequence is 59% identical to that of SNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. SNAP23 is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After relocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion. TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin. SNAP23 is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.

General readings

1. Guo, Z., Turner, C., Castle, D. Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell
surface projections regulates compound exocytosis in mast cells. Cell 94: 537-548, 1998.
2. Lazo, P. A., Nadal, M., Ferrer, M., Area, E., Hernandez-Torres, J., Nabokina, S. M., Mollinedo, F.,
Estivill, X. Genomic organization, chromosomal localization, alternative splicing, and isoforms of the
human synaptosome-associated protein-23 gene implicated in vesicle-membrane fusion
processes. Hum. Genet. 108: 211-215, 2001.
3. Mollinedo, F., Lazo, P. A. Identification of two isoforms of the vesicle-membrane fusion protein
SNAP-23 in human neutrophils and HL-60 cells. Biochem. Biophys. Res. Commun. 231: 808-812,
1997.

Immunoperoxidase of monoclonal antibody to SNAP23 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml]

H00008773-M01

SNAP23 monoclonal antibody (M01), clone 2F5-3D4. Western Blot analysis of SNAP23 expression in Raw 264.7.

H00008773-M01

Detection limit for recombinant GST tagged SNAP23 is approximately 0.03ng/ml as a capture antibody.

H00008773-M01

Western Blot analysis of SNAP23 expression in transfected 293T cell line (H00008773-T01) by SNAP23 MaxPab polyclonal antibody.Lane 1: SNAP23 transfected lysate(23.21 KDa).Lane 2: Non-transfected lysate.

H00008773-B01

SNAP23:

Western Blot analysis of SNAP23 expression in transfected 293T cell line (H00008773-T02) by SNAP23 MaxPab polyclonal antibody.Lane 1: SNAP23 transfected lysate(23.40 KDa).Lane 2: Non-transfected lysate.

H00008773-D01P

Human Jurkat; WB Suggested Anti-SNAP23 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; SNAP23 antibody - N-terminal region (ARP48615_P050) in Human Jurkat cells using Western Blot

ARP48615_P050

AP21296PU-N SNAP23 antibody staining of Human Placenta lysate at 0.1µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AP21296PU-N

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23613PU-N

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23613PU-N

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23613PU-N

Sample (30 ug of whole cell lysate). A: H1299. 12% SDS PAGE. SNAP23 antibody diluted at 1/1000

AP23613PU-N

Tonsil stained in IHC-P with SNAP23 antibody

AP55310PU-N

AP55310PU-N

TP301596

(0.2ug/ml) staining of PBMC lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA311277

Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-6: Human placenta tissue, hela cells, hepg2 cells, human fetal liver tissue, A549 cells, human normal kidney tissue, Primary antibody: (SNAP23 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds

TA350431

The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using (SNAP23 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)

TA350431

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (SNAP23 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)

TA350431

WB Suggested Anti-SNAP23 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate

TA346718

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
AP23613PU-N	SNAP23 (1-132) antibody
	Rabbit		Aff - Purified	Hu	P, WB	50 µl / €425.00
						OriGene Technologies GmbH
+3 additional images	Highly validated antibody!
AP21296PU-N	SNAP23 antibody
	Goat		Aff - Purified	Bov, Hu, Ms, Rt	E, P, WB	0.1 mg / €380.00
						OriGene Technologies GmbH
+1 additional image	OriGene recommends this product for Western blotting.
TA311277	SNAP23 (C-term) antibody
	Goat Anti-SNAP23 (aa 135-144) Antibody
	Goat		Aff - Purified	Bov, Can, Hu, Ms, Rb, Rt	E, WB	0.1 mg / €360.00
						OriGene Technologies, Inc.
TA350431	SNAP23 antibody
	Rabbit Polyclonal Anti-SNAP23 Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+2 additional images
AP55310PU-N	SNAP23 antibody
	Rabbit		Purified	Hu	E, ICC/IF, WB	0.1 mg / €450.00
						OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP301596	SNAP23 (transcript variant 1)
	Recombinant protein of human synaptosomal-associated protein, 23kDa (SNAP23), transcript variant 1
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR202271L2V	Lenti ORF particles, Snap23 (GFP-tagged) - Mouse synaptosomal-associated protein 23 (Snap23), transcript variant 3, 200 uL, >10^7 TU/mL
		200 µl / €840.00
		OriGene Technologies, Inc.

MR202271L1V	Lenti ORF particles, Snap23 (Myc-DDK-tagged) - Mouse synaptosomal-associated protein 23 (Snap23), transcript variant 3, 200 uL, >10^7 TU/mL
		200 µl / €740.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR210292L2V	Lenti ORF particles, Snap23 (GFP-tagged ORF) - Rat synaptosomal-associated protein 23 (Snap23), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

RR210292L1V	Lenti ORF particles, Snap23 (Myc-DDK-tagged ORF) - Rat synaptosomal-associated protein 23 (Snap23), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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