SP110 antibody
Principal name
SP110 antibody
Alternative names for SP110 antibody
SP110 nuclear body protein, SP-110, Speckled 110 kD, FLJ22835, VODI, IPR1
Ncbi ID
9606, NP_536349, NP_004500, NP_001171944, NP_001171944.1, NM_004509, NM_001185015, NP_780606.3, NM_175397, NM_001034137, NM_004510
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of SP110 antibody
The nuclear body is a multiprotein complex that may have a role in the regulation of gene transcription. SP110 is a member of the SP100/SP140 family of nuclear body proteins and is a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator. In addition, it has been suggested that the protein may play a role in ribosome biogenesis and in the induction of myeloid cell differentiation. The nuclear body is a multiprotein complex that may have a role in the regulation of gene transcription. This gene is a member of the SP100/SP140 family of nuclear body proteins and encodes a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator. In addition, it has been suggested that the protein may play a role in ribosome biogenesis and in the induction of myeloid cell differentiation. Altertive splicing has been observed for this gene and three transcript variants, encoding distinct isoforms, have been identified.
Immunoperoxidase of monoclonal antibody to SP110 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
Western blot analysis of SP110 over-expressed 293 cell line, cotransfected with SP110 Validated Chimera RNAi ( Cat # H00003431-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SP110 monoclonal antibody (M01), clone 8C8 (Cat # H00003431-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
SP110 monoclonal antibody (M01), clone 8C8. Western Blot analysis of SP110 expression in Jurkat ( Cat # L017V1 ).
Western Blot analysis of SP110 expression in transfected 293T cell line by SP110 monoclonal antibody (M01), clone 8C8.Lane 1: SP110 transfected lysate(61.8 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged SP110 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to SP110 on HeLa cell. [antibody concentration 10 ug/ml]
SP110 monoclonal antibody (M03), clone 2G10 Western Blot analysis of SP110 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged SP110 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to SP110 on HeLa cell. [antibody concentration 10 ug/ml]
SP110 monoclonal antibody (M02), clone 6F11 Western Blot analysis of SP110 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged SP110 is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SP110 is approximately 0.3ng/ml as a capture antibody.
Western Blot analysis of SP110 expression in transfected 293T cell line (H00003431-T01) by SP110 MaxPab polyclonal antibody.Lane 1: SP110 transfected lysate(61.80 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of human brain tissue using SP110 polyclonal antibody ( Cat # PAB13317 ) at 2.5 µg/ml.
Western blot analysis of SP110 in Hela cell lysate with SP110 polyclonal antibody ( Cat # PAB13317 ) at ( A ) 0.5, ( B ) 1, and ( C ) 2 µg/ml.
Western blot analysis of SP110 in SW480 cell lysate with SP110 polyclonal antibody ( Cat # PAB13318 ) at ( A ) 1 and ( B ) 2 µg/ml.
Human MCF7; WB Suggested Anti-SP110 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: MCF7 cell lysate. SP110 is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells.; SP110 antibody - C-terminal region (ARP36125_P050) in Human MCF7 cells using Western Blot
Human MCF7; Host:Rabbit. Target Name:SP110. Sample Tissue:Human MCF7. Antibody Dilution: 1.0ug/ml. SP110 is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells.; SP110 antibody - middle region (ARP36126_P050) in Human MCF7 cells using Western Blot
Human HT1080; WB Suggested Anti-SP110 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HT1080 cell lysate. There is BioGPS gene expression data showing that SP110 is expressed in HT1080.; SP110 antibody - middle region (ARP36126_P050) in Human HT1080 cells using Western Blot
Human HepG2; WB Suggested Anti-SP110 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; SP110 antibody - middle region (ARP37795_P050) in Human HepG2 cells using Western Blot
Tonsil, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Spleen, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807854)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SP110 (RC205648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SP110.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807900)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807900)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807900)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SP110 (RC205648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SP110.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807938)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807938)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807938)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807938)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807938)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807938)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807938)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SP110 (RC205648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SP110.
Western blot analysis of IPR1 in Hela cell lysate with IPR1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Immunohistochemical staining of human brain tissue using IPR1 antibody at 2.5 ug/ml.
Immunofluorescence of IPR1 in Human Brain cells with IPR1 antibody at 50 ug/mL.
Western blot analysis of IPR1 in SW480 cell lysate with IPR1 antibody at (A) 1 and (B) 2 µg/ml.
WB Suggested Anti-SP110 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysate
WB Suggested Anti-SP110 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: MCF7 cell lysateSP110 is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells
Host:Rabbit; Target Name:SP110; Sample Tissue:Human MCF7; Antibody Dilution: 1.0 ug/mlSP110 is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells
WB Suggested Anti-SP110 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HT1080 cell lysateThere is BioGPS gene expression data showing that SP110 is expressed in HT1080
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA807854 | SP110 antibody |
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| SP110 mouse monoclonal antibody, clone OTI4C1 (formerly 4C1) | |||||||
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Mouse | IgG1 | OTI4C1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +19 additional images |
Highly validated antibody!
|
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| TA807900 | SP110 antibody |
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| SP110 mouse monoclonal antibody, clone OTI1C3 (formerly 1C3) | |||||||
 |
Mouse | IgG1 | OTI1C3 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA807938 | SP110 antibody |
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| SP110 mouse monoclonal antibody, clone OTI2A1 (formerly 2A1) | |||||||
 |
Mouse | IgG1 | OTI2A1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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