SP140 antibody
Principal name
SP140 antibody
Alternative names for SP140 antibody
Nuclear antigen Sp-140, Speckled 140 kDa, LYSP100
SwissProt ID
Q13342 (Human)
Gene ID
11262 (SP140)
Ncbi ID
NP_001005176, 9606, NP_009168, NP_009168.4, NM_001005176, NM_007237
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection))
Background of SP140 antibody
- Kit Component:
- - KN215894G1, SP140 gRNA vector 1 in pCas-Guide vector
- KN215894G2, SP140 gRNA vector 2 in pCas-Guide vector
- KN215894D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Madani,N., et al., (2002) J. Virol. 76 (21), 11133-11138
Human kidney; Rabbit Anti-SP 140 Antibody. Catalog Number: ARP32854. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; SP140 antibody - N-terminal region (ARP32854_T100) in Human kidney cells using Immunohistochemistry
Human Raji; WB Suggested Anti-SP140 Antibody Titration: 5.0ug/ml. Positive Control: Raji cell lysate. SP140 is supported by BioGPS gene expression data to be expressed in Raji.; SP140 antibody - N-terminal region (ARP32854_T100) in Human Raji cells using Western Blot
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
SP140 Antibody (Center) (Cat. #TA324502) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SP140 antibody detected the SP140 protein (arrow).
SP140 Antibody (Center) (Cat. #TA324502)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SP140 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
IF image of Hela cell stained with SP140 Antibody (Center)(Cat#TA324502).Hela cells were incubated with SP140 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . SP140 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
WB Suggested Anti-SP140 Antibody Titration: 5.0ug/ml; Positive Control: Raji cell lysateSP140 is supported by BioGPS gene expression data to be expressed in Raji
Rabbit Anti-SP 140 Antibody; Paraffin Embedded Tissue: Human Kidney; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
TA334181 (2µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324502 | SP140 (Center) antibody |
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| Rabbit polyclonal SP140 Antibody (Center) | |||||||
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Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
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| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA334181 | SP140 antibody |
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| Goat Anti-SP140 (aa29-41) Antibody | |||||||
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Goat | Purified | Hu | WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| AM31747PU-N | SP140 antibody |
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 |
Mouse | IgG2a | 3F9 | Purified | Hu | E, P |
50 µg /
€475.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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