SPANXC antibody
Principal name
SPANXC antibody
Alternative names for SPANXC antibody
SPANX family member C, SPAN-Xc, SPANX-C, CTp11, CT11.3, Sperm protein associated with the nucleus on the X chromosome C, Cancer/testis antigen 11.3
SwissProt ID
Q9NY87 (Human)
Gene ID
64663 (SPANXC)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of SPANXC antibody
Human Sperm Proteins Associated with the Nucleus on X-chromosome (SPANX) are relatively low molecular weight cytoplasmic proteins found in testis and sperm. Their expression in other tissues indicates malignancies. Family members are observed as proteins that range from 15 to 20 kDa.
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Testis.
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Testis.
Western blot using SPANXC polyclonal antibody ( Cat # PAB11267 ) shows detection of a band at ~17 kDacorresponding to SPANXC present in a nuclear extract from VWM105 cells ( left panel, arrowhead ).VWM105 cells arederived from a human melanoma and arepositive for SPANX proteins.Lane 2 shows reactivity with a purified recombinant SPANXC fusion protein.The right panel shows similar reactivity with purified recombinant SPANXB, SPANXC and SPANXN proteins.Proteins were separatedby SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1 : 1,000.IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MX was used ( left ).IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers asindicated.Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.
Immunohistochemistry of SPANXC polyclonal antibody ( Cat # PAB11268 ) was used at 2.5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.This image shows moderate positive staining of human sperm and spermatids at 60X.Tissue was formalin-fixed and paraffin embedded.The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
Western blot using SPANXC polyclonal antibody ( Cat # PAB11268 ) shows detection of a band at ~17 kDa corresponding to SPANXC present in a nuclear extract from VWM105 cells ( left panel, arrowhead ).VWM105 cells are derived from a human melanoma and are positive for SPANX proteins.Lane 2 shows reactivity with a purified recombinant SPANXC fusion protein.The right panel shows similar reactivity with purified recombinant SPANXB, SPANXC and SPANXN proteins.Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1 : 1,000.IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MX was used ( left ).IRDye is a trademark of LI-COR, Inc.Size estimation was made by comparison to prestained MW markers as indicated.Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.
WB using Anti-SPANX (pan) antibody shows detection of a band at ~17 kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins.The primary antibody was diluted to 1:1,000.
WB using anti-SPANX-C antibody shows detection of a band at ~17 kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Primary antibody was used at 1:1,000.
affinity purified anti-SPANX-C antibody was used at 2.5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human sperm and spermatids at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA319497 | SPANX (pan) (31-47) antibody |
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| Rabbit polyclonal anti-SPANX antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Chimp, Gorilla, Hu | E, WB |
0.1 mg /
€515.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA319498 | SPANXC antibody |
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| Rabbit polyclonal anti-SPANX-C antibody | |||||||
 |
Rabbit | IgG | Purified | Chimp, Hu | E, P, WB |
0.5 mg /
€590.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| AP07638PU-N | SPANXC antibody |
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 |
Rabbit | Aff - Purified | Hu | E, P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| AP07699PU-N | SPANXC (full length) antibody |
||||||
 |
Rabbit | Purified | Hu | E, P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY411604 | SPANXC overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
