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SPATA17 antibody

Principal name

SPATA17 antibody

Alternative names for SPATA17 antibody

Spermatogenesis-associated protein 17

SwissProt ID

Q96L03 (Human), Q9D552 (Mouse)

Gene ID

128153 (SPATA17), 74717 (Spata17)

Ncbi ID

NP_620151, NP_620151.1, NM_138796, NM_029067

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of SPATA17 antibody

Spata17?gene, which called MSRG11, by digital differential display of ESTs found only in testis. The deduced 292-amino acid protein was considered to be a member of the family of calmodulin (CaM)-binding proteins because it contained 3 short CaM-binding motifs containing conserved ile and gln residues (IQ motif). Spata17 protein was most abundant in the cytoplasm of round spermatids and elongating spermatids within seminiferous tubules of the adult testis. Expression gradually decreased in the manipulated testis of animals that underwent experimental unilateral cryptorchidism. Transient transfection experiments with a Spata17 expression construct in cultured spermatogonia GC-1 cells indicated that Spata17 accelerated apoptosis in a dose-dependent manner.

Human Brain; WB Suggested Anti-SPATA17 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; SPATA17 antibody - C-terminal region (ARP52643_P050) in Human Brain cells using Western Blot

ARP52643_P050

Predicted band size: 43kDa. Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 10 minutes)

TA322067

Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using SPATA17 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200)

TA322067

Predicted band size: 43kDa. Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute)

TA322068

Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using SPATA17 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)

TA322068

WB Suggested Anti-SPATA17 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human brain

TA340310

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA322067	SPATA17 antibody
	Anti-SPATA17 rabbit polyclonal antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+1 additional image
TA322068	SPATA17 antibody
	Anti-SPATA17 rabbit polyclonal antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+1 additional image

Lysates

Catalog No.
LY408482	SPATA17 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR201840L2V	Lenti ORF particles, Spata17 (GFP-tagged) - Mouse spermatogenesis associated 17 (Spata17), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

MR201840L1V	Lenti ORF particles, Spata17 (Myc-DDK-tagged) - Mouse spermatogenesis associated 17 (Spata17), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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