SPATA22 antibody
Principal name
SPATA22 antibody
Alternative names for SPATA22 antibody
Spermatogenesis-associated protein 22, NYD-SP20
Ncbi ID
9606, NP_115987, NP_001164166.1, NM_032598, NM_001170696, NM_001170698, NP_001038996.1, NM_001170699, NM_001170695, NM_001170697
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of SPATA22 antibody
- Kit Component:
- - KN316548G1, Spata22 gRNA vector 1 in pCas-Guide vector
- KN316548G2, Spata22 gRNA vector 2 in pCas-Guide vector
- KN316548D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Huang,X., (2005) J. Androl. 26 (2), 189-196
Human 293T; WB Suggested Anti-SPATA22 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 293T cell lysate; SPATA22 antibody - N-terminal region (ARP58665_P050) in Human 293T cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPATA22 (RC205958, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPATA22.(1:500)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SPATA22 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808420)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SPATA22 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808420)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SPATA22 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808420)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SPATA22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808420)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-SPATA22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808420)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SPATA22 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808420)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SPATA22 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808420)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPATA22 (RC205958, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPATA22.(1:2000)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SPATA22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808425)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SPATA22 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808425)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SPATA22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808425)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SPATA22 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808425)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SPATA22 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808425)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPATA22 (RC205958, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPATA22.(1:2000)
WB Suggested Anti-SPATA22 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: 293T cell lysate
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA808420 | SPATA22 antibody |
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| SPATA22 mouse monoclonal antibody, clone OTI6H9 (formerly 6H9) | |||||||
 |
Mouse | IgG1 | OTI6H9 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +7 additional images |
Highly validated antibody!
|
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| TA808425 | SPATA22 antibody |
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| SPATA22 mouse monoclonal antibody, clone OTI1E2 (formerly 1E2) | |||||||
 |
Mouse | IgG1 | OTI1E2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA808417 | SPATA22 antibody |
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| SPATA22 mouse monoclonal antibody, clone OTI2B1 (formerly 2B1) | |||||||
 |
Mouse | IgG2b | OTI2B1 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760140 | SPATA22 (full length, N-term HIS tag) |
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| Recombinant protein of human spermatogenesis associated 22 (SPATA22), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY410013 | SPATA22 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY432882 | SPATA22 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. | ||
| LY432942 | SPATA22 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |