SPINK1 antibody
Principal name
SPINK1 antibody
Alternative names for SPINK1 antibody
Pancreatic secretory trypsin inhibitor, PSTI, TATI, Serine protease inhibitor Kazal-type 1, Tumor-associated trypsin inhibitor
SwissProt ID
P00995 (Human), P00996 (Bovin), P00997 (Sheep), P00998 (Pig), P04542 (Canfa), P09655 (Rat), P81634 (Horse)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of SPINK1 antibody
- Kit Component:
- - KN206574G1, SPINK1 gRNA vector 1 in pCas-Guide vector
- KN206574G2, SPINK1 gRNA vector 2 in pCas-Guide vector
- KN206574D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Witt H., et al. (2000) Nat. Genet. 25:213-216Hassan Z., et al. (2002) Am. J. Hum. Genet. 71:964-968
Immunoperoxidase of monoclonal antibody to SPINK1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 1 ug/ml]
SPINK1 monoclonal antibody (M01), clone 4D4. Western Blot analysis of SPINK1 expression in human pancreas.
SPINK1 monoclonal antibody (M01), clone 4D4. Western Blot analysis of SPINK1 expression in human pancreas.
Detection limit for recombinant GST tagged SPINK1 is approximately 3ng/ml as a capture antibody.
SPINK1 MaxPab rabbit polyclonal antibody. Western Blot analysis of SPINK1 expression in mouse kidney.
Western Blot analysis of SPINK1 expression in transfected 293T cell line (H00006690-T02) by SPINK1 MaxPab polyclonal antibody.Lane 1: SPINK1 transfected lysate(8.50 KDa).Lane 2: Non-transfected lysate.
Human MCF-7; WB Suggested Anti-SPINK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; SPINK1 antibody - N-terminal region (ARP33865_P050) in Human MCF-7 cells using Western Blot
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
SPINK1 monoclonal antibody clone 4D4. Western Blot analysis of SPINK1 expression in human pancreas.
Detection limit for recombinant GST tagged SPINK1 is 0.03 ng/ml as a capture antibody.
Representative Standard Curve
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SPINK1 Rabbit Polyclonal antibody. (TA590663)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-SPINK1 Rabbit Polyclonal antibody. (TA590663)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPINK1 (RC206574, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPINK1.
ELISA: SPINK1 Antibody
Predicted band size: 9 kDa . Positive control: GST-SPINK1 recombinant protein lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
WB Suggested Anti-SPINK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate
Western Blot: SPINK1 Antibody [TA337179] - Western blot analysis of SPINK1 in PANC-1 cell lysate in the A) absence and B) presence of immunizing peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA322899 | SPINK1 antibody |
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| Anti-SPINK1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA337179 | SPINK1 antibody |
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| Rabbit Polyclonal SPINK1 Antibody | |||||||
![Western Blot: SPINK1 Antibody [TA337179] - Western blot analysis of SPINK1 in PANC-1 cell lysate in the A) absence and B) presence of immunizing peptide.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-29620-1-W.jpg) |
Rabbit | Purified | Hu | WB |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| AM31023PU-N | SPINK1 antibody |
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 |
Mouse | IgG2a | 4D4 | Purified | Hu | E, IP, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
 +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP720447 | SPINK1 |
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| Recombinant protein of human serine peptidase inhibitor, Kazal type 1 (SPINK1) | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
ELISA Kits
| Catalog No. | ||
|---|---|---|
| EA100734 | Human SPINK1/TATI ELISA Kit |
|
 |
1 kit /
€500.00
|
|
| OriGene Technologies, Inc. |
Lysates
| Catalog No. | ||
|---|---|---|
| LY418887 | SPINK1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR204545L2V | Lenti ORF particles, Spink3 (GFP-tagged ORF) - Rat serine peptidase inhibitor, Kazal type 3 (Spink3), 200 uL, >10^7 TU/mL |
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|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR204545L1V | Lenti ORF particles, Spink3 (Myc-DDK-tagged ORF) - Rat serine peptidase inhibitor, Kazal type 3 (Spink3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||