SPRED1 antibody
Principal name
SPRED1 antibody
Alternative names for SPRED1 antibody
Spred-1, NFLS, EVH1/Sprouty domain containing protein, Suppressor of Ras/MAPK activation
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of SPRED1 antibody
- Kit Component:
- - KN220714G1, SPRED1 gRNA vector 1 in pCas-Guide vector
- KN220714G2, SPRED1 gRNA vector 2 in pCas-Guide vector
- KN220714D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Nonami A., et al. (2005) Genes Cells 10:887-895.King J.A.J., et al. (2005) Biochem. J. 388:445-454.
Formalin-fixed and paraffin-embedded human brain tissue reacted with SPRED1 polyclonal antibody ( Cat # PAB3100 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of SPRED1 polyclonal antibody ( Cat # PAB3100 ) ( arrow ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the SPRED1 gene ( Lane 2 ) ( Origene Technologies ) .
Formalin-fixed and paraffin-embedded human brain tissue reacted with SPRED1 polyclonal antibody ( Cat # PAB3102 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of SPRED1 ( arrow ) using rabbit SPRED1 polyclonal antibody ( Cat # PAB3102 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the SPRED1 gene ( Lane 2 ) ( Origene Technologies ) .
Formalin-fixed and paraffin-embedded human brain tissue reacted with SPRED1 antibody (C-term) (AP11428PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of SPRED1 Antibody (C-term) polyclonal antibody (AP11428PU-N)(arrow). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the SPRED1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human brain tissue reacted with SPRED1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of SPRED1 (arrow) using rabbit polyclonal SPRED1 Antibody (N-term) (AP11430PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the SPRED1 gene (Lane 2) (Origene Technologies).
Human Brain, cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of Spred1 in human brain tissue lysate with Spred1 antibody (AP22989PU-N) at (A) 1 and (B) 2 µg/ml.
Western blot analysis of Spred1 in mouse brain tissue lysate with Spred1 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry of Spred1 in human brain tissue with Spred1 antibody at 2.5 ug/ml.
Western blot analysis of Spred1 in human brain tissue lysate with Spred1 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry of Spred1 in human brain tissue with Spred1 antibody at 2.5 ug/ml.
Immunofluorescence of Spred1 in Human Brain tissue with Spred1 antibody at 20 ug/mL.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306657 | SPRED1 (Center) antibody |
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| Rabbit Polyclonal Spred1 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA306662 | SPRED1 (Center) antibody |
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| Rabbit Polyclonal Spred1 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| AP11428PU-N | SPRED1 (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP11430PU-N | SPRED1 (N-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP22989PU-N | SPRED1 antibody |
||||||
 |
Rabbit | Purified | Hu, Ms, Rt | P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207088L2V | Lenti ORF particles, Spred1 (GFP-tagged) - Mouse sprouty protein with EVH-1 domain 1, related sequence (Spred1), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,100.00
|
|
| OriGene Technologies, Inc. | ||
| MR207088L1V | Lenti ORF particles, Spred1 (Myc-DDK-tagged) - Mouse sprouty protein with EVH-1 domain 1, related sequence (Spred1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€990.00
|
|
| OriGene Technologies, Inc. | ||