SQSTM1 antibody
Principal name
SQSTM1 antibody
Alternative names for SQSTM1 antibody
Sequestosome-1, EBIAP, ORCA, OSIL, p60, p62, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBI3-associated protein of 60 kDa
Ncbi ID
NP_001135770, 9606, NP_001135771, NP_003891, NP_001135770.1, NM_003900, NM_001142298, BC006019, NM_011018, NM_175843, NM_181550, NM_001142299
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Flow Cytometry (F)
Background of SQSTM1 antibody
p62/SQSTM1 (sequestosome 1) is ubiquitously-expressed cytoplasmic/adapter protein conserved in vertebrates and it can be induced by proteasomal inhibitor PSI, PGJ2/prostaglandin J2 as well as phorbol 12-myristate 13-acetate (PMA). SQSTM1 has the ability of ubiquitin binding as well as regulating NFkB1 activation by TNF-alpha, NGF (nerve growth factor) and interleukin-1. SQSTM1 acts as an adapter that mediates the interaction between TRAF6 and CYLD, and SQSTM1-TRAF6 interaction leads to K63-linked polyubiquitition of TRAF6 followed by subsequent activation of NFkB pathway. SQSTM1 plays a role in TITIN/TTN downstream sigling in muscle cells and regulate sigling cascades through ubiquitition. SQSTM1 is essential both for formation and autophagic degradation of polyubiquitin-containing bodies which are known as aggresome-like induced structures (ALIS) and SQSTM1 connects ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members. Moreover, while autophagy modulates SQSTM1 protein levels, SQSTM1 suppresses autophagy via activation of mTORC1. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress. SQSTM1 functions as a sigling hub for various sigl transduction pathways, apoptosis, cell differentiation, apoptosis, immune response, regulation of K+ channels and Nrf2 activation, and its dysregulation is associated with Paget disease of bone and tumorigenesis.
General readings
Falchetti A., et al. (2004) J Bone Miner Res. 19(6):1013-7.
Immunofluorescence of monoclonal antibody to SQSTM1 on HeLa cell. [antibody concentration 10 ug/ml]
Detection limit for recombinant GST tagged SQSTM1 is approximately 0.03ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with SQSTM1 polyclonal antibody ( Cat # PAB1750 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
Immunofluorescence staining of SQSTM1 polyclonal antibody ( Cat # PAB1750 ) on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
Western blot analysis of SQSTM1 ( arrow ) using SQSTM1 polyclonal antibody ( Cat # PAB1750 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the SQSTM1 gene ( Lane 2 ) ( Origene Technologies ) .
Detection limit for recombinant GST tagged SQSTM1 is approximately 3ng/ml as a capture antibody.
Western Blot analysis of SQSTM1 expression in transfected 293T cell line (H00008878-T02) by SQSTM1 MaxPab polyclonal antibody.Lane 1: SQSTM1 transfected lysate(47.70 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab antibody to SQSTM1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of SQSTM1 expression in transfected 293T cell line (H00008878-T01) by SQSTM1 MaxPab polyclonal antibody.Lane 1: SQSTM1 transfected lysate(48.4 KDa).Lane 2: Non-transfected lysate.
Recombinant human SQSTM1, 1-356 aa, His-tagged: 15% SDS-PAGE (3 µg)
Human Pancreas; Pancreas; SQSTM1 antibody - middle region (ARP54262_P050) in Human Pancreas cells using Immunohistochemistry
Hum. Fetal Heart; Host: Rabbit . Target Name: SQSTM1 . Sample Tissue: Human Fetal Heart . Antibody Dilution: 1.0ug/ml.; SQSTM1 antibody - middle region (ARP54262_P050) in Hum. Fetal Heart cells using Western Blot
Hum. Fetal Liver; Host: Rabbit . Target Name: SQSTM1 . Sample Tissue: Human Fetal Liver . Antibody Dilution: 1.0ug/ml.; SQSTM1 antibody - middle region (ARP54262_P050) in Hum. Fetal Liver cells using Western Blot
Hum. Fetal Lung; Host: Rabbit . Target Name: SQSTM1 . Sample Tissue: Human Fetal Lung . Antibody Dilution: 1.0ug/ml.; SQSTM1 antibody - middle region (ARP54262_P050) in Hum. Fetal Lung cells using Western Blot
Human Pancreas; WB Suggested Anti-SQSTM1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Pancreas; SQSTM1 antibody - middle region (ARP54262_P050) in Human Pancreas cells using Western Blot
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of SQSTM1 in Human spleen tissue lysate with SQSTM1 antibody at (A) 1 and (B) 2 µg/ml.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502127)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502127)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502127)
Anti-SQSTM1 mouse monoclonal antibody (TA502127) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1(RC203214).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502127), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502128)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502128)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502128)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502128)
Anti-SQSTM1 mouse monoclonal antibody (TA502128) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1(RC203214).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502128), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502129)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502129)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502129)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502129)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502130)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502130)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502130)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502130)
Anti-SQSTM1 mouse monoclonal antibody (TA502130) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1(RC203214).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502130), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502131)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502131)
Anti-SQSTM1 mouse monoclonal antibody (TA502131) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1(RC203214).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502131), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502132)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502132)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502132)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502132)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502132)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502132)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502132), and then analyzed by flow cytometry.
Anti-SQSTM1 mouse monoclonal antibody (TA502133) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1(RC203214).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody.
Flow cytometric Analysis of Hela cells, using anti-SQSTM1 antibody(TA502133),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502133), and then analyzed by flow cytometry.
Anti-SQSTM1 mouse monoclonal antibody (TA502174) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1(RC203214).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody.
Flow cytometric Analysis of Hela cells, using anti-SQSTM1 antibody(TA502174),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502174), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502199), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502238)
Anti-SQSTM1 mouse monoclonal antibody (TA502238) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1(RC203214).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502238), and then analyzed by flow cytometry.
Anti-SQSTM1 mouse monoclonal antibody (TA502239) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1(RC203214).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody.
Figure from citation: Western Blot of SQSTM1(p62) protein level by using anti-SQSTM1 antibody in human HEp-2 cell lysates. View Citation
HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502239), and then analyzed by flow cytometry.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SQSTM1 mouse monoclonal antibody (Clone UMAB13). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-SQSTM1 (Clone UMAB13) very specifically recognizes SQSTM1 antigen on OriGene protein microarray chip.
Western blot analysis of SQSTM1 Antibody (C331) Pab (Cat.#TA300177) pre-incubated without(lane 1) and with(lane 2) blocking peptide in MCF-7 cell line lysate. SQSTM1 Antibody (C331) (arrow) was detected using the purified Pab.
Western blot analysis of SQSTM1 (arrow) using rabbit polyclonal Autophagy SQSTM1 (p62) Antibody (C-term ) (Cat.#TA300177). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Immunofluorescence staining of Autophagy SQSTM1 (p62) Antibody (C-term) (Cat# TA300177) on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
IF image of U251 cells stained with SQSTM1 (p62) (C-term) antibody. U251 cells were treated with Chloroquine , then incubated with TA300177 SQSTM1 (p62) (C-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . SQSTM1 (p62) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Western blot analysis of SQSTM1 in Human spleen tissue lysate with SQSTM1 antibody at (A) 1 and (B) 2 µg/ml.
Immunohistochemistry of SQSTM1 in rat spleen tissue with SQSTM1 antibody at 5 ug/ml.
Immunofluorescence of SQSTM1 in Rat Spleen cells with SQSTM1 antibody at 20 ug/mL.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with polyclonal anti-p62 antibody (Clone Poly6477). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
Western Blot: p62/SQSTM1 Antibody [TA336759] - Analysis of SQSTM1 in HeLa whole cell extracts
Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [TA336759] - Confocal immunofluorescent analysis of HeLa cells using p62/SQSTM1 antibody (TA336759, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green).
Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [TA336759] - SQSTM1 staining in of Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).
Western Blot: p62/SQSTM1 Antibody [TA336760] - Analysis of SQSTM1 in HeLa whole cell extracts.
Western Blot: p62/SQSTM1 Antibody [TA336760] - p62 levels in control and drug treated CHLA-15 neuroblastoma cells. Image received from verified customer review.
Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [TA336760] - staining in of Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).
Western Blot: p62/SQSTM1 Antibody [TA336780] - Analysis of SQSTM1/p62 in HeLa whole cell lysates
Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [TA336780] - Immunocytochemical analysis of SQSTM1/p62 in HeLa cells
Western Blot: p62/SQSTM1 Antibody (5H7E2) [TA336949] - Western blot analysis using p62/SQSTM1 mAb against human SQSTM1 recombinant protein. (Expected MW is 39.1 kDa)
Western Blot: p62/SQSTM1 Antibody (5H7E2) [TA336949] - Western blot analysis using p62/SQSTM1 mAb in (1) HEK293 and (2) SQSTM1 hIgGFc transfected HEK293 cell lysate.
Western Blot: p62/SQSTM1 Antibody (5H7E2) [TA336949] - Western blot analysis using p62/SQSTM1 mouse mAb in (1) Hela, (2) Jurkat, (3) THP-1, (4) HEK293, (5) A549, (6) MCF-7, (7) HepG2, (8) COS7 and (9) SK-BR-3 cell lysate.
Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) [TA336949] - IHC analysis of paraffin-embedded bladder cancer tissues using SQSTM1 mouse mAb with DAB staining.
Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) [TA336949] - IHC analysis of paraffin-embedded human cervical cancer tissues using p62/SQSTM1 mouse mAb with DAB staining.
Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) [TA336949] - p62/SQSTM1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Flow Cytometry: p62/SQSTM1 Antibody (5H7E2) [TA336949] - Flow cytometry analysis of HEK293 cells using SQSTM1 mouse mAb (green) and negative control (red).
Western blot analysis of P62/SQSTM1 expression inHeLa
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- Highly Validated (16)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502239 | SQSTM1 antibody |
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| SQSTM1 mouse monoclonal antibody, clone OTI6A7 (formerly 6A7) | |||||||
 |
Mouse | IgG2a | OTI6A7 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Published recently! Highly validated antibody!
|
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| UM500012 | SQSTM1 antibody |
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| SQSTM1 mouse monoclonal antibody,clone UMAB12 | |||||||
 |
Mouse | IgG2b | UMAB12 | Purified | Hu | P, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +17 additional images |
Highly validated antibody!
|
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| UM500013 | SQSTM1 antibody |
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| SQSTM1 mouse monoclonal antibody, clone UMAB13 | |||||||
 |
Mouse | IgG1 | UMAB13 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| UM570012 | SQSTM1 antibody |
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| SQSTM1 mouse monoclonal antibody,clone UMAB12 | |||||||
|
Mouse | IgG2b | UMAB12 | Purified | Hu | P, WB |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +17 additional images |
Highly validated antibody!
|
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| UM570013 | SQSTM1 antibody |
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| SQSTM1 mouse monoclonal antibody, clone UMAB13 | |||||||
|
Mouse | IgG1 | UMAB13 | Purified | Hu | F, ICC/IF, WB |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA300177 | SQSTM1 (C-term) antibody |
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| Rabbit Polyclonal Antibody against SQSTM1 | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody! |
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| TA336949 | SQSTM1 antibody |
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| Mouse Monoclonal p62/SQSTM1 Antibody (5H7E2) | |||||||
![Western Blot: p62/SQSTM1 Antibody (5H7E2) [TA336949] - Western blot analysis using p62/SQSTM1 mAb against human SQSTM1 recombinant protein. (Expected MW is 39.1 kDa)](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-23490-1-W.jpg) |
Mouse | IgG1 | 5H7E2 | Purified | Hu, Prim | C, E, F, ICC/IF, P, WB |
0.1 ml /
€395.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody! |
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| TA502127 | SQSTM1 antibody |
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| SQSTM1 mouse monoclonal antibody, clone OTI1E9 (formerly 1E9) | |||||||
 |
Mouse | IgG2b | OTI1E9 | Purified | Hu, Mky, Rt | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA502128 | SQSTM1 antibody |
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| SQSTM1 mouse monoclonal antibody, clone OTI4A12 (formerly 4A12) | |||||||
 |
Mouse | IgG1 | OTI4A12 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| TA502129 | SQSTM1 antibody |
||||||
| SQSTM1 mouse monoclonal antibody, clone OTI5D5 (formerly 5D5) | |||||||
 |
Mouse | IgG2b | OTI5D5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA502130 | SQSTM1 antibody |
||||||
| SQSTM1 mouse monoclonal antibody, clone OTI4E2 (formerly 4E2) | |||||||
 |
Mouse | IgG1 | OTI4E2 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| TA502131 | SQSTM1 antibody |
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| SQSTM1 mouse monoclonal antibody, clone OTI5A2 (formerly 5A2) | |||||||
 |
Mouse | IgG1 | OTI5A2 | Purified | Hu, Mky | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA502132 | SQSTM1 antibody |
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| SQSTM1 mouse monoclonal antibody, clone OTI4G9 (formerly 4G9) | |||||||
 |
Mouse | IgG1 | OTI4G9 | Purified | Hu, Mky | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
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| TA502133 | SQSTM1 antibody |
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| SQSTM1 mouse monoclonal antibody, clone OTI4C8 (formerly 4C8) | |||||||
 |
Mouse | IgG2a | OTI4C8 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA502174 | SQSTM1 antibody |
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| SQSTM1 mouse monoclonal antibody, clone OTI7F6 (formerly 7F6) | |||||||
 |
Mouse | IgG1 | OTI7F6 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA502238 | SQSTM1 antibody |
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| SQSTM1 mouse monoclonal antibody, clone OTI5A1 (formerly 5A1) | |||||||
 |
Mouse | IgG2a | OTI5A1 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA306861 | SQSTM1 (C-term) antibody |
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| Rabbit Polyclonal SQSTM1 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA336759 | SQSTM1 antibody |
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| Rabbit Polyclonal p62/SQSTM1 Antibody | |||||||
![Western Blot: p62/SQSTM1 Antibody [TA336759] - Analysis of SQSTM1 in HeLa whole cell extracts](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP1-42821-1-W.jpg) |
Rabbit | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€395.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA336760 | SQSTM1 antibody |
||||||
| Rabbit Polyclonal p62/SQSTM1 Antibody | |||||||
![Western Blot: p62/SQSTM1 Antibody [TA336760] - Analysis of SQSTM1 in HeLa whole cell extracts.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP1-42822-1-W.jpg) |
Rabbit | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€395.00
|
||
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA336780 | SQSTM1 antibody |
||||||
| Rabbit Polyclonal p62/SQSTM1 Antibody | |||||||
![Western Blot: p62/SQSTM1 Antibody [TA336780] - Analysis of SQSTM1/p62 in HeLa whole cell lysates](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP1-48320-1-W.jpg) |
Rabbit | Purified | Hu, Ms, Rt | C, ICC/IF, WB |
0.1 ml /
€475.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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