SSX1 antibody
Principal name
SSX1 antibody
Alternative names for SSX1 antibody
Synovial sarcoma X breakpoint 1, CT5.1, Cancer/testis antigen 5.1
SwissProt ID
Q16384 (Human)
Gene ID
6756 (SSX1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of SSX1 antibody
- Kit Component:
- - KN201600G1, SSX1 gRNA vector 1 in pCas-Guide vector
- KN201600G2, SSX1 gRNA vector 2 in pCas-Guide vector
- KN201600D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Crew A J., et al. (1995) EMBO J. 14:2333-2340.Nilsson G., et al. (1999) Cancer Res. 59:3180-3184.
Human OVCAR-3; WB Suggested Anti-SSX1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: OVCAR-3 cell lysate; SSX1 antibody - middle region (ARP57900_P050) in Human OVCAR-3 cells using Western Blot
Western blot analysis of SSX-pan expression in HEK293 (A); MDAMB453 (B); SW480 (C); A549 (D) whole cell lysates.
Anti-SSX1 mouse monoclonal antibody (TA502523) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX1(RC201600).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX1 (RC201600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX1.
HEK293T cells transfected with either RC201600 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SSX1 antibody(TA502523), and then analyzed by flow cytometry.
Anti-SSX1 mouse monoclonal antibody (TA502693) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX1(RC201600).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX1 (RC201600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX1.
HEK293T cells transfected with either RC201600 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SSX1 antibody(TA502693), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-SSX1 antibody(TA502693),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SSX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502722)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SSX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502722)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SSX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502722)
Anti-SSX1 mouse monoclonal antibody (TA502722) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX1(RC201600).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX1 (RC201600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX1.
Flow cytometric Analysis of Jurkat cells, using anti-SSX1 antibody(TA502722),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-SSX1 antibody(TA502722),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC201600 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SSX1 antibody(TA502722), and then analyzed by flow cytometry.
Anti-SSX1 mouse monoclonal antibody (TA502724) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX1(RC201600).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX1 (RC201600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSX1 monoclonal antibody.
Flow cytometric Analysis of Hela cells, using anti-SSX1 antibody(TA502724),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-SSX1 antibody(TA502724),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC201600 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SSX1 antibody(TA502724), and then analyzed by flow cytometry.
Anti-SSX1 mouse monoclonal antibody (TA502726) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX1(RC201600).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX1 (RC201600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX1.
HEK293T cells transfected with either RC201600 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SSX1 antibody(TA502726), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-SSX1 antibody(TA502726),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-SSX1 mouse monoclonal antibody (TA502728) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX1(RC201600).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX1 (RC201600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX1.
HEK293T cells transfected with either RC201600 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SSX1 antibody(TA502728), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-SSX1 antibody(TA502728),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-SSX1 antibody(TA502728),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SSX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502730)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SSX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502730)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SSX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502730)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SSX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502730)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SSX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502730)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SSX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502730)
Anti-SSX1 mouse monoclonal antibody (TA502730) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX1(RC201600).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX1 (RC201600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX1.
Flow cytometric Analysis of Hela cells, using anti-SSX1 antibody(TA502730),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC201600 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SSX1 antibody(TA502730), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-SSX1 antibody(TA502730),(Red), compared to a nonspecific negative control antibody,(Blue).
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: K562 cells, Primary antibody: (SSX1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
- Quick-Filters:
- Highly Validated (6)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502693 | SSX1 antibody |
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| SSX1 mouse monoclonal antibody, clone OTI1B5 (formerly 1B5) | |||||||
 |
Mouse | IgG1 | OTI1B5 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
||||||
| TA502722 | SSX1 antibody |
||||||
| SSX1 mouse monoclonal antibody, clone OTI1E10 (formerly 1E10) | |||||||
 |
Mouse | IgG1 | OTI1E10 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
||||||
| TA502724 | SSX1 antibody |
||||||
| SSX1 mouse monoclonal antibody, clone OTI2E9 (formerly 2E9) | |||||||
 |
Mouse | IgG1 | OTI2E9 | Purified | Can, Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA502726 | SSX1 antibody |
||||||
| SSX1 mouse monoclonal antibody, clone OTI2G5 (formerly 2G5) | |||||||
 |
Mouse | IgG1 | OTI2G5 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA502728 | SSX1 antibody |
||||||
| SSX1 mouse monoclonal antibody, clone OTI2D12 (formerly 2D12) | |||||||
 |
Mouse | IgG1 | OTI2D12 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
| TA502730 | SSX1 antibody |
||||||
| SSX1 mouse monoclonal antibody, clone OTI2F8 (formerly 2F8) | |||||||
 |
Mouse | IgG1 | OTI2F8 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +10 additional images |
Highly validated antibody!
|
||||||
| TA350450 | SSX1 antibody |
||||||
| Rabbit Polyclonal Anti-SSX1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA502523 | SSX1 antibody |
||||||
| SSX1 mouse monoclonal antibody, clone OTI1D10 (formerly 1D10) | |||||||
 |
Mouse | IgG1 | OTI1D10 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301600 | SSX1 |
||||
| Recombinant protein of human synovial sarcoma, X breakpoint 1 (SSX1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY417166 | SSX1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |