STAT3 antibody
Principal name
STAT3 antibody
Alternative names for STAT3 antibody
STAT-3, Acute-phase response factor, APRF, Signal transducer and activator of transcription 3
SwissProt ID
P40763 (Human), P42227 (Mouse), P52631 (Rat), P61635 (Bovin), Q19S50 (Pig), Q6DV79 (Chick)
Ncbi ID
NP_003141.2, 9606, 10090, 10116, NP_003141, NP_644805, NM_213662, NM_139276, NP_998827, NM_213660, NM_011486, NM_012747
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Dot blot (Dot)
Background of STAT3 antibody
STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon alpha (IFN alpha) and IFN gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5.
Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage.
STAT1 and STAT2 are induced by IFN alpha and forn 1, a heterodimer which is part of the ISGF transcription factor complex. The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNa, IL10, IL6, IL11, IL12, IL2, EGF, among others. STAT3 beta appears to be activated by both EGF and IL6, but STAT3 alpha is only activated by EGF. STAT3 binds to DNA as either a homodimer or as a heterodimer with STAT1. STAT3 interacts with the APRE acute phase response element) and SIE (csis inducible element). STAT4, predominantly expressed in testis and myeloid cells, is activated by ILl2. STAT5 is activated by prolactin and IL3. STAT6 participates in signaling pathways mediated by IL4.
General readings
1. Akira, S., Nishio, Y., Inoue, M., Wang, X.-J., Wei, S., Matsusaka, T., Yoshida, K., Sudo, T., Naruto,
M., Kishimoto, T. Molecular cloning of APRF, a novel IFN-stimulated gene factor 3 p91-related
transcription factor involved in the gp130-mediated signaling pathway. Cell 77: 63-71, 1994.
2. Bhattacharya, S., Schindler, C. Regulation of Stat3 nuclear export. J. Clin. Invest. 111: 553-559,
2003.
3. Bong, Y.-S., Lee, H.-S., Carim-Todd, L., Mood, K., Nishanian, T. G., Tessarollo, L., Daar, I.
O. ephrinB1 signals from the cell surface to the nucleus by recruitment of STAT3. Proc. Nat. Acad.
Sci. 104: 17305-17310, 2007.
Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) HEPG2 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab STAT3-9E12 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Phosphospecificity Whole cell extracts of control (1), EGF stimulated (2) or pervanadate treated (3) A 431 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab STAT3-23G5 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 0 sec).
Proximity Ligation Analysis (PLA) of protein-protein interactions between STAT1 and STAT3 HeLa cells were stained with anti-STAT1 rabbit purified polyclonal 1:1200 and anti-STAT3 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
STAT3 monoclonal antibody (M01), clone 1D11-2A11. Western Blot analysis of STAT3 expression in K-562 ( Cat # L009V1 ).
STAT3 monoclonal antibody (M01), clone 1D11-2A11 Western Blot analysis of STAT3 expression in HeLa ( Cat # L013V1 ).
Proximity Ligation Analysis (PLA) of protein-protein interactions between NFKB1 and STAT3 HeLa cells were stained with anti-NFKB1 rabbit purified polyclonal 1:1200 and anti-STAT3 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Immunofluorescence of monoclonal antibody to STAT3 on HeLa cell. [antibody concentration 10 ug/ml]
STAT3 monoclonal antibody (M02), clone 4D6 Western Blot analysis of STAT3 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged STAT3 is approximately 0.1ng/ml as a capture antibody.
Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using STAT3 (Phospho-Tyr705) Antibody Cat.-No AP02344PU
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (phospho-Tyr705) Antibody Cat.-No AP02344PU
Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with IFN using STAT3 (Phospho-Tyr705) Antibody Cat.-No AP02344PU
Western blot analysis of extracts from Hela cells untreated or treated with IFN a using STAT3 (Phospho-Tyr705) Antibody Cat.-No AP02344PU
Western blot analysis of extracts from HeLa cells using STAT3 (phospho-Tyr705) Antibody Cat.-No AP02344PU
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using STAT3 (pSer727) antibody (left) or the same antibody preincubated with blocking peptide (right)
Immunofluorescence staining of methanol-fixed Hela cells using STAT3 (pSer727) Antibody
Western Blot analysis of extracts from Hela and HepG2 cells untreated or treated with PMA using STAT3 )pSer727) Antibody
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from HeLa cells using STAT3 antibody.
Immunohistochemistry analysis of STAT3 Antibody (Cat.-No AP06327PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot analysis of STAT3 Antibody (Cat.-No AP06327PU-N) at 1/500 dilution:
Lane 1: HepG2 whole cell lysate treated with IFN-alpha.
Lane 2: Raw264.7 whole cell lysate treated with IFN-alpha.
Lane 3: H9C2 whole cell lysate treated with IFN-alpha.
Lane 1: HepG2 whole cell lysate treated with IFN-alpha.
Lane 2: Raw264.7 whole cell lysate treated with IFN-alpha.
Lane 3: H9C2 whole cell lysate treated with IFN-alpha.
Immunohistochemistry (IHC) analyzes of STAT3 antibody (Cat.-No.: AP06328PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of Stat3 antibody (Cat.-No.: AP06328PU-N) at 1/500 dilution
Lane1: A549 whole cell lysate
Lane2: LO2 whole cell lysate treated with UV
Lane3: INH-3T3 whole cell lysate
Lane4: sp2/0 whole cell lysate treated with H2O2
Lane5: PC12 whole cell lysate
Lane6: H9C2 whole cell lysate
Lane1: A549 whole cell lysate
Lane2: LO2 whole cell lysate treated with UV
Lane3: INH-3T3 whole cell lysate
Lane4: sp2/0 whole cell lysate treated with H2O2
Lane5: PC12 whole cell lysate
Lane6: H9C2 whole cell lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with STAT3 (phospho S727) polyclonal antibody ( Cat # PAB0615 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
The STAT3 (phospho S727) polyclonal antibody ( Cat # PAB0615 ) is used in western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before ( - ) or after ( + ) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute ( La Jolla, CA ).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with STAT3 (phospho Y705) polyclonal antibody ( Cat # PAB0616 ) which was peroxidase-conjugated to the secondary antibody followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of STAT3 (phospho Y705) polyclonal antibody ( Cat # PAB0616 ) in mouse liver tissue lysate ( 35 µg/lane ). Phospho-STAT3 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ).
The STAT3 (phospho S727) polyclonal antibody ( Cat # PAB0617 ) is used in Western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before ( - ) or after ( + ) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute ( La Jolla, CA ).
Immunoblot analysis of cell lysates. Lane 1 : Hela cell lysate and lane 2 : Hela cells lysate treated with IFN alpha.
STAT3 polyclonal antibody ( Cat # PAB6030 ) overnight staining at 10 µg/ml on 4%PFA-fixed mouse thymus. Biotinylated mouse anti-goat then Streptavidin-AP then BCIP/NBT. Data kindly provided by Dr. Marella de Bruijn, MRC Mol. Haematol. Unit, WIMM, JR Hospital, Oxford.
STAT3 polyclonal antibody ( Cat # PAB6030 ) staining ( 0.5 µg/ml ) of A-431 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
STAT3 polyclonal antibody ( Cat # PAB7090 ) ( 1 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot (WB) analysis of Stat3 antibody (Cat.-No.: AP01233PU-N in extracts from LOVO cells.
Western blot (WB) analysis of Stat3 antibody (Cat.-No. AP01233PU-N) in extracts from HELA cells.
Immunohistochemistry (IHC) analyzes of STAT3 perS727 antibody (Cat.-No.: AP01691PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of STAT3 pSer727 antibody (Cat.-No.: AP01691PU-N) in extracts from HeLa cells.
Immunohistochemistry (IHC) analyzes of STAT3 pTyr705 antibody (Cat.-No.: AP01692PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of STAT3 pTyr705 antibody (Cat.-No.: AP01692PU-N) in extracts from HeLa cells.
The phospho-STAT3-Ser727 antibody is used in Western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before (-) or after (+) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
The Phospho-STAT3-S727 Pab (Cat#AP12717PU-N) is used in western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before (-) or after (+) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-Phospho-STAT3 Pab(Cat. #AP12718PU-N) in mouse liver tissue lysate (35ug/lane). Phospho-STAT3(arrow) was detected using the purified Pab (1:60 dilution).
Western blot analysis using STAT3 monoclonal antibody, clone 198Q22 ( Cat # MAB2023 ), on human HeLa (1) and mouse L929 (2) cell lysates. Antibodyused at 1 µg/ml.
Western Blot analysis of STAT3 expression in transfected 293T cell line (H00006774-T01) by STAT3 MaxPab polyclonal antibody.Lane 1: STAT3 transfected lysate(88.10 KDa).Lane 2: Non-transfected lysate.
The whole cell lysate derived from A-431 was immunoblotted by STAT3 ( phospho S727 ) polyclonal antibody ( Cat # PAB12753 ) at 1 : 500.
Dot Blot : 1 µg peptide was blot onto NC membrane.A : STAT3 ( pS727 , Phosphospecific ).B : STAT3 ( Paired S727, Non phospho-speicfic ).Followed were blotted at a 1 : 2000 dilution by :1 : STAT3 polyclonal antibody ( Cat # PAB12784 ).2 : STAT3 ( phospho S727 ) polyclonal antibody ( Cat # PAB12753 ).
The whole cell lysate derived from A-431 with EGF stimulated ( Lane 1 ) or without EGF stimulated ( Lane 2 ) were immunoblotted by STAT3 ( phospho Y705 ) polyclonal antibody ( Cat # PAB12754 ) at 1 : 500.
Dot Blot : 1 µg peptide was blot onto NC membrane.A : STAT3 ( pY705, Phospho-specific ).B : STAT3 ( Paired Y705, Non phosphospeicfic ).Followed were blotted at a 1 : 2000 dilution by :1. STAT3 ( phospho Y705 ) polyclonal antibody ( Cat # PAB12754 ).2. Rabbit anti-STAT3 ( Paired Y705 ).
The whole cell lysate derived from A-431 was immunoblotted by STAT3 polyclonal antibody ( Cat # PAB12784 ) at 1 : 500.
Dot Blot : 1 µg peptide was blot onto NC membrane.A : STAT3 ( pS727 ) ( Phosphospecific )B : STAT3 ( Paired S727 ) ( Nonphosphospeicfic )were blotted at a 1 : 2000 dilution by :1 : STAT3 polyclonal antibody ( Cat # PAB12784 ),2 : Phospho-STAT3 S727 polyclonal antibody ( Cat # PAB12753 ).
Western blot analysis of Stat3 expression in lysate from Jurkat cells ( Lane 1 ) , NIH/3T3 cells ( Lane 2 ) and rat kidney ( Lane 3 ) .
HeLa cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with STAT3 polyclonal antibody ( Cat # PAB13773 ).
Human kidney; Immunohistochemistry with Human kidney lysate tissue; STAT3 antibody - N-terminal region (ARP38253_P050) in Human kidney cells using Immunohistochemistry
Human Uterus; Immunohistochemistry with Human Uterus lysate tissue; STAT3 antibody - N-terminal region (ARP38253_P050) in Human Uterus cells using Immunohistochemistry
human prostate; Immunohistochemistry with human prostate tissue tissue; STAT3 antibody - N-terminal region (ARP38253_P050) in human prostate cells using Immunohistochemistry
Mouse; Immunofluorescence --. Sample Type: Macrophages. Dilution:. 1:1000; STAT3 antibody - N-terminal region (ARP38253_P050) in Mouse cells using Immunohistochemistry
Human Liver; WB Suggested Anti-STAT3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Liver; STAT3 antibody - N-terminal region (ARP38253_P050) in Human Liver cells using Western Blot
Human THP-1; WB Suggested Anti-STAT3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; STAT3 antibody - middle region (ARP30317_P050) in Human THP-1 cells using Western Blot
Human Uterus; STAT3 antibody - middle region (AVARP09057_P050) in Human Uterus cells using Immunohistochemistry
Human Thymus; Immunohistochemistry with Thymus tissue at an antibody concentration of 5.0ug/ml using anti-STAT3 antibody (AVARP09057_P050); STAT3 antibody - middle region (AVARP09057_P050) in Human Thymus cells using Immunohistochemistry
Human 721_B; WB Suggested Anti-STAT3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: 721_B cell lysate. STAT3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; STAT3 antibody - middle region (AVARP09057_P050) in Human 721_B cells using Western Blot
Immunohistochemistry (IHC) analyzes of p-STAT3 (pSer727) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of p-STAT3 (pSer727) pAb in extracts from HeLa cells.
Immunohistochemistry (IHC) analyzes of STAT3 pAb in paraffin-embedded human lung carcinoma tissue.
Western blot (WB) analyzes of STAT3 pAb in extracts from HeLa cells.
STAT3 Polyclonal Antibody
AP26348PU-N: Western blot analysis of Stat3 in lysate from Jurkat cells (Lane 1 & 2), 3T3 cells (Lane 3) and rat kidney (Lane 4).
Immunohistochemical analysis of paraffin-embedded human Liver tissues using STAT3 antibody Cat.-No AM06296SU-N
Western blot analysis using STAT3 antibody Cat.-No AM06296SU-N against full-length STAT3-His recombinant protein (1).
Immunohistochemical analysis of paraffin-embedded mammary cancer tissues (left) and lung cancer tissues (right) using STAT3 antibody Cat.-No AM06536SU-N with DAB staining.
Immunofluorescence analysis of Hela cells using STAT3 antibody Cat.-No AM06536SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using STAT3 antibody Cat.-No AM06536SU-N against Hela (1),NIH/3T3 (2), Jurkat (3), PC-12 (4) and COS7 (5) cell lysate.
Western blot analysis of STAT3 phosphorylation using AM26475AF-N in 293T cells transfected with zebrafish STAT3 (1), 293T cells co-transfected with zebrafish STAT3 and mouse JAK1 (2), 293T cells transfected with mouse STAT3 (3), 293T cells cotransfected with mouse STAT3 and mouse JAK1 (4).
HepG2 cell extracts were resloved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-STAT3 pY705 (clone 13A3-1) antibody. Proteins were visualized using an anti-mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection. Lane 1, lysates from untreated HepG2 cells; Lane 2, lysates from HepG2 cells treated with recombinant hIL-6 for 15 minutes.
Immunohistochemical analysis of STAT3 (pS727) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of STAT3 (pS727) expression in HeLa (A); Raw264.7 LPS-treated (B) whole cell lysates.
Immunohistochemical analysis of STAT3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of STAT3 expression in HepG2 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
Immunohistochemical analysis of STAT3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of STAT3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of STAT3 expression in A549 (A); LO2 (B); SP2/0 (C); NIH3T3 (D); PC12 (E); H9C2 (F) whole cell lysates.
Immunohistochemical analysis of STAT3 (pY705) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of STAT3 (pY705) expression in A431 EGF-treated (A); MCF7 (B); HeLa (C) whole cell lysates.
Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded breast within the normal limits using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded colon within the normal limits using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded thyroid within the normal limits using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT3(RC212394) (RC212394, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT3.
TA302716 (1µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA302716Streptavidin-AP then BCIP/NBT. Data kindly provided by Dr. Marella de Bruijn, MRC Mol. Haematol. Unit, WIMM, JR Hospital, Oxford.
TA303135 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of STAT3 in human small intestine tissue lysate with STAT3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of STAT3 in human small intestine tissue with STAT3 antibody at 5 ug/mL.
Immunofluorescence of STAT3 in human small intestine tissue with STAT3 antibody at 20 ug/mL.
STAT3 Antibody (pS727) (Cat. #TA324629) western blot analysis in Daudi cell line lysates (35ug/lane).This demonstrates the STAT3 antibody detected the STAT3 protein (arrow).
IF image of SY5Y cells stained with STAT3 (C-term S727) antibody. SY5Y cells were incubated with TA324629 STAT3 (C-term S727) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . Note the highly specific localization of the STAT3 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000168610).
Western blot analysis of STAT3 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of STAT3 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of STAT3 expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts from HeLa/3T3 cells, using STAT3 (Ab-705) Antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using STAT3 (Ab-705) Antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts of Untreated and treted by IFN, using Phospho-STAT3-Y705 antibody.
Western Blot: STAT3 [p Tyr705] Antibody [TA336974] - Analysis of STAT3 (Tyr705) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 705 and 2) an unphosphorylated tyrosine at position 705, using STAT3 phospho antibody at 0.1
Western blot detection of STAT3 in CHO-K1,C6,3T3,K562,CEM,Jurkat,Hela,MCF7,COS7,293T,A431 and A549 cell lysates using STAT3 mouse mAb (1:1000 diluted).Predicted band size:88KDa.Observed band size:88KDa.
Western blot analysis of STAT3 expression in Hela (A); NIH3T3 (B); PC12 (C) whole cell lysates.
Immunohistochemical analysis of STAT3 staining in human colon cancer;mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of STAT3 expression in Hela (A); mouse heart (B); rat heart (C) whole cell lysates.
Immunohistochemical analysis of STAT3 staining in human colon cancer;mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of STAT3 expression in 293T (A) whole cell lysates.
Immunohistochemical analysis of STAT3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
HepG2 cell extracts were resloved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-STAT3 pY705 (clone 13A3-1) antibody. Proteins were visualized using an anti-mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection. Lane 1, lysates from untreated HepG2 cells; Lane 2, lysates from HepG2 cells treated with recombinant hIL-6 for 15 minutes.
Western blot analysis of extracts from HeLa cells, using STAT3 (phospho-Tyr705) antibodyThe lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using STAT3 (phospho-Tyr705) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of STAT3 phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of STAT3 phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of STAT3 phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
The Phospho-STAT3-S727 Pab (Cat. #3260a) is used in western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before (-) or after (+) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).
The phospho-STAT3-Ser727 Pab (Cat. #TA324777) is used in Western blot to detect Ser727-phosphorylated STAT3 in HL-60 tissue lysates collected before (-) or after (+) stimulation obtained by IFN-a.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
IF image of SY5Y cells stained with phospho-STAT3-S727 antibody. SY5Y cells were incubated with TA324777 phospho-STAT3-S727 primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . Note the highly specific localization of the phospho-STAT3 immunosignal mainly to the nucleus.
The phospho-STAT3-Ser727 Pab (Cat. #TA302174) is used in Western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before (-) or after (+) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).
The phospho-STAT3-Ser727 Pab (Cat. #TA302174) is used in Western blot to detect Ser727-phosphorylated STAT3 in HL-60 tissue lysates collected before (-) or after (+) stimulation obtained by IFN-a.
Confocal immunofluorescent analysis of Phospho-STAT3-S727 Antibody(Cat#TA302174) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
IF image of SY5Y cells stained with phospho-STAT3-S727 antibody. SY5Y cells were incubated with TA302174 phospho-STAT3-S727 primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . Note the highly specific localization of the phospho-STAT3-S727 immunosignal to the nucleus and cytoplasm.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM26475AF-N | STAT3 pTyr705 (703-714) antibody |
||||||
 |
Mouse | IgG1 | PS3/1 | Azide Free | Hu, Ms, Ze | WB |
0.1 mg /
€320.00
|
| OriGene Technologies GmbH | |||||||
