STEAP4 antibody
Principal name
STEAP4 antibody
Alternative names for STEAP4 antibody
STEAP-4, STAMP2, TNFAIP9, Metalloreductase STEAP4, Six-transmembrane epithelial antigen of prostate 4, SixTransMembrane protein of prostate 2, Tumor necrosis factor, alpha-induced protein 9
Ncbi ID
9606, NP_078912, NP_001192244.1, NP_001192244, NM_024636, NP_473439.2, NM_001044265
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of STEAP4 antibody
- Kit Component:
- - KN316858G1, Steap4 gRNA vector 1 in pCas-Guide vector
- KN316858G2, Steap4 gRNA vector 2 in pCas-Guide vector
- KN316858D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Wellen KE, Fucho R, Gregor MF, Furuhashi M, Morgan C, Lindstad T, Vaillancourt E, Gorgun CZ, Saatcioglu F, Hotamisligil GS.
Coordinated regulation of nutrient and inflammatory responses by STAMP2 is essential for metabolic homeostasis. 2007 May 4;129(3):537-48.
PMID: 17482547
293 overexpressing STEAP4 and probed with STEAP4 polyclonal antibody ( Cat # PAB7348 ) ( mock transfection in first lane), tested by Origene.
STEAP4 polyclonal antibody ( Cat # PAB7348 ) ( 0.3 µg/ml ) staining of human adipose lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Human Jurkat; WB Suggested Anti-STEAP4 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; STEAP4 antibody - C-terminal region (ARP49842_P050) in Human Jurkat cells using Western Blot
Western blot analysis of STEAP4 in human prostate tissue lysate with STEAP4 polyclonal antibody ( Cat # PAB13009 ) at ( A ) 1 and ( B ) 2 µg/ml.
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of HeLa cells, using STEAP4 Antibody. The picture on the right is treated with the synthesized peptide.
TA302619 (0.3µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of STEAP4 in rat liver tissue lysate with STEAP4 antibody at (A) 0.5 and (B) 1 µg/ml.
Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using STEAP4 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of Jurkat cells, using STEAP4 antibody.The picture on the right is treated with the synthesized peptide.
Anti-STEAP4 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Predicted cell location: Cytoplasm . Positive control: Human gastric and lung cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using STEAP4 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm . Positive control: Human gastric and lung cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using STEAP4 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm . Positive control: Human esophagus and cervical cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using STEAP4 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm . Positive control: Human esophagus and cervical cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using STEAP4 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)
WB Suggested Anti-STEAP4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate
Anti-STEAP4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302619 | STEAP4 (C-term) antibody |
||||||
| Goat Polyclonal Antibody against STEAP4 / Dudulin4 | |||||||
 |
Goat | Aff - Purified | Can, Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA306466 | STEAP4 (Center) antibody |
||||||
| Rabbit Polyclonal STEAP4 Antibody | |||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€415.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA312418 | STEAP4 antibody |
||||||
| Rabbit polyclonal STEAP4 antibody [#C11207] | |||||||
 |
Rabbit | IgG | Purified | Hu | E, ICC/IF, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA324150 | STEAP4 antibody |
||||||
| Anti-STEAP4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA324151 | STEAP4 antibody |
||||||
| Anti-STEAP4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA341238 | STEAP4 antibody |
||||||
| Rabbit Polyclonal Anti-STEAP4 Antibody (N-Terminus) | |||||||
 |
Rabbit | Purified | Hu, Mky |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AP23641PU-N | STEAP4 antibody |
||||||
 |
Rabbit | IgG | Hu | E, ICC/IF, P |
50 µl /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP316917 | STEAP4 |
||||
| Recombinant protein of human STEAP family member 4 (STEAP4) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403020 | STEAP4 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR215648L2V | Lenti ORF particles, Steap4 (GFP-tagged ORF) - Rat STEAP family member 4 (Steap4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,150.00
|
|
| OriGene Technologies, Inc. | ||
| RR215648L1V | Lenti ORF particles, Steap4 (Myc-DDK-tagged ORF) - Rat STEAP family member 4 (Steap4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,020.00
|
|
| OriGene Technologies, Inc. | ||