STIM2 antibody
Principal name
STIM2 antibody
Alternative names for STIM2 antibody
Stromal interaction molecule 2, KIAA1482
Ncbi ID
NP_001162588.1, NP_065911, 9606, NP_001162588, NP_001074572.2, NM_001081103, NM_020860, NM_001169117, NM_001169118
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of STIM2 antibody
Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including syptic transmission, muscle contraction, hormol secretion, cell growth and proliferation. The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become the focus of intense research. Recently, several key players of the store-operated complex have been identified: the Orai family consists of three members: Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor. While STIM1 appears to be localized intracellularly at the ER membrane and at a much lower extent on the cell surface of several cell types, STIM2 appears to be localized solely at the ER membrane. The function of STIM2 still remains elusive and several models have been suggested.
Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE)
Formalin fixed, paraffin embedded human spleen stained with Rabbit anti Human STIM2 (AP05701PU-N) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
Western blot analysis of whole cell lysate from A20 mouse B-cell lymphoma cells probed with Rabbit anti Human STIM2 (AP05701PU-N) at 0.5(A), 1(B) and 2(C) µg/ml
Immunohistochemistry of STIM2 in human spleen tissue with STIM2 polyclonal antibody ( Cat # PAB13265 ) at 2.5 µg/ml.
Western blot analysis of STIM2 in A-20 cell lysate with STIM2 polyclonal antibody ( Cat # PAB13265 ) at ( A ) 0.5 and ( B ) 1 µg/ml.
Immunohistochemistry of STIM2 in human spleen tissue with STIM2 polyclonal antibody ( Cat # PAB13266 ) at 2.5 µg/ml.
Western blot analysis of STIM2 in A-20 cell lysate with STIM2 polyclonal antibody ( Cat # PAB13266 ) at ( A ) 0.5, ( B ) 1 and ( C ) 2 µg/ml.
Human Small Intestine (formalin-fixed, paraffin-embedded) stained with STIM2 antibody AP22869PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Pancreas (formalin-fixed, paraffin-embedded) stained with STIM2 antibody AP22869PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Liver (formalin-fixed, paraffin-embedded) stained with STIM2 antibody AP22869PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis for AP22869PU-N of STIM2 in A-20 cell lysate with at (A) 0.5, (B) 1 and (C) 2 µg/ml.
Formalin fixed, paraffin embedded human kidney tissue stained with STIM2 Antibody (N-term) Cat.-No AP54073PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of STIM2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of STIM2 Antibody (N-term) Cat.-No AP54073PU-N in UM cell line lysates (35ug/lane). This demonstrates the STIM2 antibody detected the STIM2 protein (arrow
Western blot analysis of STIM2 in A-20 cell lysate with STIM2 antibody at (A) 0.5 and (B) 1 ug/ml.
Immunohistochemistry of STIM2 in human spleen tissue with STIM2 antibody at 2.5 ug/ml.
Immunofluorescence of STIM2 in Human Spleen cells with STIM2 antibody at 20 ug/mL.
Western blot analysis of STIM2 in A-20 cell lysate with STIM2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Immunohistochemistry of STIM2 in human spleen tissue with STIM2 antibody at 2.5 ug/ml.
Immunofluorescence of STIM2 in Human Spleen cells with STIM2 antibody at 20 ug/mL.
Western blot analysis of rat brain (lanes 1,3) and RBL cell (lanes 2,4) lysates: 1. Anti-STIM2 antibody, (1:200). 2. Anti-STIM2 antibody, preincubated with the control peptide antigen.
Expression of STIM2 in rat lung. Immunohistochemical staining of paraffin embedded rat lung sections using Anti-STIM2 antibody, (1:100). Staining is present in the respiratory epithelium of the bronchiole (red) as well as in the pneumonocytes of the alveolar wall (blue). Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the counterstain.
Expression of STIM2 in RBL cells. Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat basophilic leukemia (RBL) cells. A, D. Cells stained with Anti-STIM2 antibody, (1:100) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B, E. Nuclear staining of cells using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B F. Merged image of panels D and E.
Host: Rabbit; Target Name: Stim2; Sample Tissue: Mouse Pancreas lysates; Antibody Dilution: 1.0 ug/ml
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- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP22869PU-N | STIM2 antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | E, P, WB |
50 µg /
€425.00
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| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA306422 | STIM2 (C-term) antibody |
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| Rabbit Polyclonal STIM2 Antibody | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA306423 | STIM2 (Center) antibody |
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| Rabbit Polyclonal STIM2 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA328646 | STIM2 antibody |
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| Rabbit Polyclonal Anti-STIM2 | |||||||
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Rabbit | Purified | Hu, Ms, Rt | WB |
0.2 ml /
€585.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AP05701PU-N | STIM2 antibody |
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€330.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP07232PU-N | STIM2 antibody |
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Rabbit | Purified | Hu, Ms, Rt | P, WB |
50 µg /
€425.00
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| OriGene Technologies GmbH | |||||||
| AP54073PU-N | STIM2 (N-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP324102 | STIM2 |
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| Recombinant protein of human stromal interaction molecule 2 (STIM2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR219434L2V | Lenti ORF particles, Stim2 (GFP-tagged) - Mouse stromal interaction molecule 2 (Stim2), 200 uL, >10^7 TU/mL |
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200 µl /
€1,540.00
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| OriGene Technologies, Inc. | ||