STIP1 antibody
Principal name
STIP1 antibody
Alternative names for STIP1 antibody
Stress-induced-phosphoprotein 1, STI1, Hop, NY-REN-11, Hsc70/Hsp90-organizing protein, Transformation-sensitive protein IEF SSP 3521
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), EPair for Elisa (EPair)
Background of STIP1 antibody
STIP1 is an adaptor protein that coordites the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM, Jul 2009]. Transcript Variant: This variant (2) uses an alterte 5' termil exon and thus differs in the 5' UTR and 5' coding region, and uses an alterte start codon, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additiol publications. ##Evidence-Data-START## Transcript exon combition :: BC002987.1, M86752.1 [ECO:0000332] Rseq introns :: single sample supports all introns ERS025081, ERS025082 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
General readings
Van Der Spuy J., et al. (2001) Biochem J. 21:462-469.
Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.3 ug/ml]
Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml]
STIP1 monoclonal antibody (M01), clone 4B6 Western Blot analysis of STIP1 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged STIP1 is approximately 0.1ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml]
STIP1 monoclonal antibody (M11), clone 1E3 Western Blot analysis of STIP1 expression in HeLa ( Cat # L013V1 ).
STIP1 monoclonal antibody (M11), clone 1E3. Western Blot analysis of STIP1 expression in NIH/3T3 ( Cat # L018V1 ).
STIP1 monoclonal antibody (M11), clone 1E3. Western Blot analysis of STIP1 expression in Raw 264.7 ( Cat # L024V1 ).
STIP1 monoclonal antibody (M11), clone 1E3. Western Blot analysis of STIP1 expression in PC-12 ( Cat # L012V1 ).
Detection limit for recombinant GST tagged STIP1 is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml]
STIP1 MaxPab polyclonal antibody. Western Blot analysis of STIP1 expression in human liver.
Western Blot analysis of STIP1 expression in transfected 293T cell line (H00010963-T01) by STIP1 MaxPab polyclonal antibody.Lane 1: STIP1 transfected lysate(59.73 KDa).Lane 2: Non-transfected lysate.
STIP1 MaxPab polyclonal antibody. Western Blot analysis of STIP1 expression in HeLa.
Human Jurkat; WB Suggested Anti-STIP1 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate. STIP1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; STIP1 antibody - N-terminal region (ARP46164_T100) in Human Jurkat cells using Western Blot
Human Muscle; STIP1 antibody - C-terminal region (ARP46165_T100) in Human Muscle cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-STIP1 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate. STIP1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; STIP1 antibody - C-terminal region (ARP46165_T100) in Human Jurkat cells using Western Blot
Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human ovary cancer. [antibody concentration 0.75 ug/ml]
FACS analysis of A-431 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained A-431 cells (Black) as negative control.
FACS analysis of TOV-21G cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained TOV-21G cells (Black) as negative control.
FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained ES-2 cells (Black) as negative control.
FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained 293 cells (Black) as negative control.
FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained HeLa cells (Black) as negative control.
Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human ovary cancer. [antibody concentration 0.75 ug/ml]
FACS analysis of A-431 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained A-431 cells (Black) as negative control.
FACS analysis of TOV-21G cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained TOV-21G cells (Black) as negative control.
FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.
FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained 293 cells (Black) as negative control.
FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.
Immunofluorescence of purified MaxPab antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml]
STIP1 MaxPab polyclonal antibody. Western Blot analysis of STIP1 expression in human liver.
Western Blot analysis of STIP1 expression in transfected 293T cell line (H00010963-T02) by STIP1 MaxPab polyclonal antibody.Lane 1: STIP1 transfected lysate(59.73 KDa).Lane 2: Non-transfected lysate.
STIP1 MaxPab polyclonal antibody. Western Blot analysis of STIP1 expression in HeLa.
Recombinant human Stress-induced-phosphoprotein 1 (1- 543 aa), His-tagged: 15% SDS-PAGE (3 µg)
STIP1 antibody detects STIP1 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole
Sample(30 ug of whole cell lysate). A:Hep G2. B:MOLT4. 7.5% SDS PAGE. TA308465 diluted at 1:5000
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using STIP1(TA308465) antibody at 1:100 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using STIP1(TA308465) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
Western blot analysis of lysates from H-4-II-E, mouse NIH/3T3 cell line, mouse brain and rat brain tissues (from left to right), using STIP1 Antibody (C-term) (Cat. #TA324735). TA324735 was diluted at 1:1000 at each lane. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
STIP1 Antibody (C-term) (Cat. #TA324735) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
STIP1 Antibody (Center) (Cat.# TA324736) western blot analysis in A431,A549 cell line lysates (35ug/lane).This demonstrates the STIP1 antibody detected the STIP1 protein (arrow).
Formalin-fixed and paraffin-embedded human testis tissue reacted with STIP1 Antibody (Center) (Cat.# TA324736), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
STIP1 Antibody (Center) (Cat. #TA324736) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of Jurkat cell lysate using STIP1 antibody.
Immunohistochemistry of paraffin-embedded rat liver using STIP1 antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded rat spleen using STIP1 antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of HepG2 cell using STIP1 antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-STIP1 Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate.STIP1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
WB Suggested Anti-STIP1 Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate.STIP1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
Human Muscle
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308465 | STIP1 antibody |
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| Rabbit Polyclonal antibody to STIP1 (stress-induced-phosphoprotein 1) | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 ml /
€415.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA327005 | STIP1 antibody |
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| STIP1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA324735 | STIP1 (C-term) antibody |
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| Rabbit polyclonal STIP1 Antibody (C-term) | |||||||
 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA324736 | STIP1 antibody |
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| Rabbit polyclonal STIP1 Antibody (Center) | |||||||
 |
Rabbit | IgG | Purified | Bov, Hst, Hu, Ms, Rt | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301084 | STIP1 |
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| Recombinant protein of human stress-induced-phosphoprotein 1 (STIP1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP710087 | STIP1 (full length, N-term His tag) |
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| Recombinant protein of human human stress-induced-phosphoprotein 1 (STIP1), full length, with N-terminal His tag, expressed in sf9 cells | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
€411.00
|
|
| OriGene Technologies, Inc. | |||||
| TP710122 | STIP1 (full length, C-term DDK tag) |
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| Recombinant protein of human human stress-induced-phosphoprotein 1 (STIP1), full length, with C-terminal DDK tag, expressed in sf9 cells | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
€411.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208668L2V | Lenti ORF particles, Stip1 (GFP-tagged) - Mouse stress-induced phosphoprotein 1 (Stip1), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR208668L1V | Lenti ORF particles, Stip1 (Myc-DDK-tagged) - Mouse stress-induced phosphoprotein 1 (Stip1), 200 uL, >10^7 TU/mL |
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200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201985L2V | Lenti ORF particles, Stip1 (GFP-tagged ORF) - Rat stress-induced phosphoprotein 1 (Stip1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,250.00
|
|
| OriGene Technologies, Inc. | ||
| RR201985L1V | Lenti ORF particles, Stip1 (Myc-DDK-tagged ORF) - Rat stress-induced phosphoprotein 1 (Stip1), 200 uL, >10^7 TU/mL |
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200 µl /
€1,120.00
|
|
| OriGene Technologies, Inc. | ||