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STK11 antibody

Principal name

STK11 antibody

Alternative names for STK11 antibody

Serine/threonine-protein kinase 11, LKB1, PJS, NY-REN-19, Serine/threonine-protein kinase LKB1, Renal carcinoma antigen NY-REN-19

SwissProt ID

Q0GGW5 (Chick), Q15831 (Human), Q91604 (Xenla), Q9WTK7 (Mouse)

Gene ID

6794 (STK11), 20869 (Stk11)

Ncbi ID

9606, NP_000446.1, NP_000446, AAH07981, NP_000446.1 (Gene ID : 6794), NM_000455, AAH19334, NM_011492

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C)

Background of STK11 antibody

Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, leading to promote their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2: it thereby regulates inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neurons polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1 Also acts as a mediator p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways.

General readings

Xie,Z., (2006) J. Biol. Chem. 281 (10), 6366-6375

Formalin-fixed and paraffin-embedded human cancer tissue reacted with STK11 polyclonal antibody ( Cat # PAB2804 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.

PAB2804

Immunofluorescence of purified MaxPab antibody to STK11 on HeLa cell. [antibody concentration 10 ug/ml]

H00006794-B01P

Western Blot analysis of STK11 expression in transfected 293T cell line (H00006794-T01) by STK11 MaxPab polyclonal antibody.Lane 1: STK11 transfected lysate(47.63 KDa).Lane 2: Non-transfected lysate.

H00006794-B01P

Proximity Ligation Analysis (PLA) of protein-protein interactions between STK11 and PRKAA2 HeLa cells were stained with anti-STK11 rabbit purified polyclonal 1:1200 and anti-PRKAA2 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00006794-D01P

Immunofluorescence of purified MaxPab rabbit antibody to STK11 on HeLa cell. [antibody concentration 10 ug/ml]

H00006794-D01P

Western Blot analysis of STK11 expression in transfected 293T cell line (H00006794-T01) by STK11 MaxPab polyclonal antibody.Lane 1: STK11 transfected lysate(48.60 KDa).Lane 2: Non-transfected lysate.

H00006794-D01P

Human Skeletal muscle; Rabbit Anti-STK11 Antibody. Catalog Number: ARP41743. Paraffin Embedded Tissue: Human Muscle. Cellular Data: Skeletal muscle cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; STK11 antibody - N-terminal region (ARP41743_T100) in Human Skeletal muscle cells using Immunohistochemistry

ARP41743_T100

Human Jurkat; WB Suggested Anti-STK11 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate. STK11 is supported by BioGPS gene expression data to be expressed in Jurkat.; STK11 antibody - N-terminal region (ARP41743_T100) in Human Jurkat cells using Western Blot

ARP41743_T100

Western blot analysis of STK11 Antibody (N-term) (AP13928PU-N) in Ramos cell line lysates. STK11 was detected using the purified Pab.

AP13928PU-N

Western blot analysis of STK11 in PC-3 cell lysate with STK11 polyclonal antibody ( Cat # PAB12979 ) at 1 µg/ml in the ( A ) absence or ( B ) presence of blocking peptide.

PAB12979

Western blot (WB) analysis of LKB1 antibody (Cat.-No.: AP20255PU-N) in extracts from raw264.7 cells.

AP20255PU-N

Immunohistochemistry (IHC) analyzes of p-LKB1 antibody (Cat.-No.: AP20912PU-N) in paraffin-embedded human lung carcinoma tissue.

AP20912PU-N

Immunohistochemistry analysis of p-LKB1 Antibody (Cat.-No AP20913PU-N) in paraffin-embedded human breast carcinoma tissue.

AP20913PU-N

Western blot analysis of p-LKB1 Antibody (Cat.-No AP20913PU-N) in extracts from CV-1 forsklin cells.

AP20913PU-N

Immunohistochemistry (IHC) analyzes of LKB1 antibody (Cat.-No.: AP21003PU-N) in paraffin-embedded human lung carcinoma tissue.

AP21003PU-N

Western blot (WB) analyzes of LKB1 antibody (Cat.-No.: AP21003PU-N) in extracts from CV-1cells.

AP21003PU-N

Immunohistochemistry (IHC) analyzes of LKB1 antibody (Cat.-No.: AP21004PU-N) in paraffin-embedded human breast carcinoma tissue.

AP21004PU-N

Western blot (WB) analyzes of LKB1 antibody (Cat.-No.: AP21004PU-N) in extracts from CV-1cells.

AP21004PU-N

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using LKB1 (Phospho-Ser428) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of NIH-3T3 cells, using LKB1 (Phospho-Ser428) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30', using LKB1 (Phospho-Ser428) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from NIH-3T3 cells treated with PMA 125ng/ml 30', using LKB1 (Phospho-Thr189) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using LKB1 (Ab-428) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of NIH-3T3 cells, using LKB1 (Ab-428) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells, treated with Serum 20% 15', using LKB1 (Ab-189) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HT-29 cells, using LKB1 (Ab-334) Antibody. The lane on the right is treated with the synthesized peptide.

Human HepG2; WB Suggested Anti-STK11 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; STK11 antibody - C-terminal region (ARP58959_P050) in Human HepG2 cells using Western Blot

ARP58959_P050

Western blot analysis using STK11 antibody Cat.-No AM06552SU-N against NIH/3T3 (1),Raw246.7 (2), COS7 (3), Jurkat (4), HEK293 (5) and A431 (6) cell lysate.

AM06552SU-N

Flow Cytometric analysis of K562 cells using STK11 antibody Cat.-No AM06552SU-N (green) and negative control (purple).

AM06552SU-N

Western blot analysis of extracts from HeLa cells treated with PMA using Phospho-LKB1 (Ser428) antibody AP55929PU-N.The lane on the right is treated with the antigen-specific peptide.

Western blot analysis of LKB1 (pS428) expression in HeLa UV-treated (A); NIH3T3 UV-treated (B); mouse brain (C); PC12 UV-treated (D) whole cell lysates.

Immunohistochemical analysis of LKB1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of LKB1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of LKB1 expression in A431 (A); DLD (B); Raw264.7 (C); PC12 (D) whole cell lysates.

Western blot analysis of LKB1 expression in Jurkat (A); K562 (B); NIH3T3 (C) whole cell lysates.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STK11 (RC202885, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK11.

TA802377

TA802379

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-STK11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802554)

TA802554

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-STK11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802554)

TA802554

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-STK11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802554)

TA802554

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-STK11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802554)

TA802554

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-STK11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802554)

TA802554

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-STK11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802554)

TA802554

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-STK11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802554)

TA802554

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-STK11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802554)

TA802554

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-STK11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802554)

TA802554

TA802554

Formalin-fixed and paraffin-embedded human cancer tissue (breast carcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

TA300047

Western blot analysis of STK11 (arrow) using rabbit polyclonal STK11 Antibody (AP13926PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the STK11 gene (Lane 2) (Origene Technologies).

TA300047

Western blot analysis of LKB1 in PC-3 cell lysate with LKB1 antibody at 1 µg/ml in the (A) absence or (B) presence of blocking peptide.

TA306371

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using LKB1 antibody.The picture on the right is treated with the synthesized peptide.

TA313336

Immunofluorescence analysis of NIH/3T3 cells, using LKB1 antibody.The picture on the right is treated with the synthesized peptide.

TA313336

Western blot analysis of extracts from HeLa cells, using OR2B8 antibody.The lane on the right is treated with the synthesized peptide.

TA313337

Anti-STK11 / LKB1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

TA317798

Anti-STK11 / LKB1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

TA317798

Western blot analysis of LKB1 expression in Serum treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325910

Western blot analysis of extracts of variouscell lines , using STK11 antibody.

TA327284

Immunofluorescence analysis of MCF7 cell using STK11 antibody. Blue: DAPI for nuclear staining.

TA327284

Western Blot: LKB1 Antibody [TA336904] - LKB1 antibody was tested in human fetal liver lysate.

TA336904

Immunohistochemistry-Paraffin: LKB1 Antibody [TA336904] - LKB1 antibody was tested in mouse stomach using DAB with hematoxylin counterstain.

TA336904

Immunocytochemistry/Immunofluorescence: LKB1 Antibody [TA336904] - LKB1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

TA336904

Western Blot: LKB1 Antibody [TA336905] - LKB1 antibody was tested in human testes lysate.

TA336905

Immunohistochemistry-Paraffin: LKB1 Antibody [TA336905] - LKB1 antibody was tested in mouse stomach using DAB with hematoxylin counterstain.

TA336905

Immunocytochemistry/Immunofluorescence: LKB1 Antibody [TA336905] - LKB1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

TA336905

Western blot analysis of LKB1 phosphorylation expression in PMA treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325912

Western blot analysis of LKB1 phosphorylation expression in PMA treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325911

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA802554	STK11 antibody
	STK11 mouse monoclonal antibody, clone OTI0A3 (formerly 0A3)
	Mouse	IgG1	OTI0A3	Purified	Hu	P, WB	0.1 ml / €360.00
							OriGene Technologies, Inc.
+9 additional images	Highly validated antibody!
TA300047	STK11 (N-term) antibody
	STK11 Antibody (N-term I29) Rabbit Polyclonal Antibody (Pab)
	Rabbit	Ig		Purified	Hu	P, WB	0.4 ml / €415.00
							OriGene Technologies, Inc.
+1 additional image
TA306371	STK11 antibody
	Rabbit Polyclonal LKB1 Antibody
	Rabbit	IgG		Aff - Purified	Hu, Ms	E, WB	0.1 mg / €415.00
							OriGene Technologies, Inc.
TA313336	STK11 antibody
	Rabbit polyclonal anti-LKB1 antibody
	Rabbit	IgG		Purified	Hu, Ms	E, ICC/IF, P	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA313337	STK11 antibody
	Rabbit polyclonal anti-OR2B8 antibody
	Rabbit	IgG		Purified	Hu	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA325910	STK11 antibody
	Rabbit Polyclonal LKB1 Antibody
	Rabbit	IgG		Purified	Hu, Ms	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA325911	STK11 antibody
	Rabbit Polyclonal LKB1 (Ser428) Antibody (Phospho-specific)
	Rabbit	IgG		Purified	Hu, Ms	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA325912	STK11 antibody
	Rabbit Polyclonal LKB1 (Thr189) Antibody (Phospho-specific)
	Rabbit	IgG		Purified	Hu, Ms	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA327284	STK11 antibody
	STK11 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA327895	STK11 antibody
	Rabbit Polyclonal LKB1 Antibody
	Rabbit	IgG		Purified	Hu, Ms	P	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA336904	STK11 antibody
	Rabbit Polyclonal LKB1/STK11 Antibody
	Rabbit			Purified	Hu, Ms	C, ICC/IF, P, WB	0.1 ml / €395.00
							OriGene Technologies, Inc.
+2 additional images
TA336905	STK11 antibody
	Rabbit Polyclonal LKB1/STK11 Antibody
	Rabbit			Purified	Hu, Ms	C, ICC/IF, P, WB	0.1 ml / €415.00
							OriGene Technologies, Inc.
+2 additional images
TA802377	STK11 antibody
	STK11 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)
	Mouse	IgG1	OTI1F10	Purified	Hu	WB	0.1 ml / €360.00
							OriGene Technologies, Inc.

TA802379	STK11 antibody
	STK11 mouse monoclonal antibody, clone OTI4B2 (formerly 4B2)
	Mouse	IgG1	OTI4B2	Purified	Hu	WB	0.1 ml / €360.00
							OriGene Technologies, Inc.

AM06552SU-N	STK11 antibody
	Mouse	IgG1	4H12	Ascites	Hu, Mky, Ms	E, F, WB	0.1 ml / €380.00
							OriGene Technologies GmbH
+1 additional image
AP05211PU-N	STK11 antibody
	Rabbit	IgG		Purified	Hu	E, WB	0.1 mg / €540.00
							OriGene Technologies GmbH
AP13928PU-N	STK11 (N-term) antibody
	Rabbit	Ig		Purified	Hu	WB	0.4 ml / €415.00
							OriGene Technologies GmbH
AP20255PU-N	STK11 antibody
	Rabbit			Aff - Purified	Hu, Ms	WB	0.1 mg / €370.00
							OriGene Technologies GmbH
AP20912PU-N	STK11 pSer334 antibody
	Rabbit			Aff - Purified	Hu, Ms	P	0.1 mg / €370.00
							OriGene Technologies GmbH
AP20913PU-N	STK11 pSer428 antibody
	Rabbit			Aff - Purified	Hu	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+1 additional image