STK33 antibody
Principal name
STK33 antibody
Alternative names for STK33 antibody
Serine/threonine-protein kinase 33,
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Frozen Sections (C)
Background of STK33 antibody
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
Function: Serine/threonine protein kinase which phosphorylates VIME. May play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME (By similarity). Not essential for the survival of KRAS-dependent AML cell lines.
Tissue Location: Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system.
Immunofluorescence of monoclonal antibody to STK33 on HeLa cell. [antibody concentration 25 ug/ml]
Western blot analysis of STK33 over-expressed 293 cell line, cotransfected with STK33 Validated Chimera RNAi ( Cat # H00065975-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STK33 monoclonal antibody (M02) clone 6G1 (Cat # H00065975-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of STK33 expression in transfected 293T cell line by STK33 monoclonal antibody (M02), clone 6G1.Lane 1: STK33 transfected lysate(57.9 KDa).Lane 2: Non-transfected lysate.
STK33 monoclonal antibody (M02), clone 6G1 Western Blot analysis of STK33 expression in HeLa ( Cat # L013V1 ).
Immunofluorescence of monoclonal antibody to STK33 on HeLa cell. [antibody concentration 10 ug/ml]
STK33 monoclonal antibody (M03), clone 4A12 Western Blot analysis of STK33 expression in HeLa ( Cat # L013V1 ).
STK33 monoclonal antibody (M06), clone 4F7 Western Blot analysis of STK33 expression in Jurkat ( Cat # L017V1 ).
Immunoperoxidase of monoclonal antibody to STK33 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1.5 ug/ml]
Immunofluorescence of monoclonal antibody to STK33 on HeLa cell. [antibody concentration 10 ug/ml]
STK33 monoclonal antibody (M07), clone 3F10 Western Blot analysis of STK33 expression in Jurkat ( Cat # L017V1 ).
Immunofluorescence of monoclonal antibody to STK33 on A-431 cell. [antibody concentration 10 ug/ml]
STK33 monoclonal antibody (M09), clone 1F10. Western Blot analysis of STK33 expression in NIH/3T3(Cat # L018V1 ).
STK33 monoclonal antibody (M09), clone 1F10 Western Blot analysis of STK33 expression in A-431 ( Cat # L015V1 ).
STK33 monoclonal antibody (M09), clone 1F10. Western Blot analysis of STK33 expression in Raw 264.7(Cat # L024V1 ).
STK33 monoclonal antibody (M09), clone 1F10. Western Blot analysis of STK33 expression in PC-12(Cat # L012V1 ).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with STK33 polyclonal antibody ( Cat # PAB3392 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Western blot analysis of STK33 polyclonal antibody ( Cat # PAB3392 ) in NCI-H460 cell lysate. STK33 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot analysis of STK33 expression in transfected 293T cell line (H00065975-T02) by STK33 MaxPab polyclonal antibody.Lane 1: STK33 transfected lysate(57.90 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-STK33 Pab (Cat#AP14426PU-N) in NCI-H460 cell lysate. STK33 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
STK33 monoclonal antibody (M06), clone 4F7 Western Blot analysis of STK33 expression in Jurkat ( Cat # L017V1 ).
Western Blot analysis of STK33 expression in transfected 293T cell line (H00065975-T01) by STK33 MaxPab polyclonal antibody.Lane 1: STK33 transfected lysate(56.54 KDa).Lane 2: Non-transfected lysate.
STK33 MaxPab polyclonal antibody. Western Blot analysis of STK33 expression in Jurkat.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to STK33 on HeLa cell (antibody concentration 10 µg/ml).
STK33 monoclonal antibody, clone 3F10 Western Blot analysis of STK33 expression in Jurkat.
STK33 Antibody (N-term) (Cat. #AP54076PU-N) western blot analysis in Jurkat cell line lysates (35µg/lane).This demonstrates the STK33 antibody detected the STK33 protein (arrow).
Immunohistochemical analysis of STK33 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of STK33 expression in A431 (A); Jurkat (B); HeLa (C) whole cell lysates.
Western Blot: STK33 Antibody - WB detection of Stk33 in HEK293 cell lysate.
Immunohistochemistry: STK33 Antibody - IHC staining of STK33 in mouse testis.
Western blot analysis of extracts from HeLa/293 cells, using STK33 antibody.The lane on the right is treated with the synthesized peptide.
Host: Rabbit; Target Name: STK33; Sample Tissue: THP-1 Whole Cell lysates; Antibody Dilution: 1.0ug/ml
WB Suggested Anti-STK33 Antibody; Titration: 1.0 ug/ml; Positive Control: MCF7 Whole Cell
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM21008PU-N | STK33 antibody |
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Mouse | IgG1 | 3F10 | Hu | E, ICC/IF, P, WB |
50 µg /
€475.00
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| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA309885 | STK33 antibody |
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| Rabbit Polyclonal STK33 Antibody | |||||||
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Rabbit | Purified | Hu, Ms | C, ICC/IF, WB |
0.1 ml /
€395.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA312422 | STK33 antibody |
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| Rabbit polyclonal anti-STK33 antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA350458 | STK33 antibody |
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| Rabbit Polyclonal Anti-STK33 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP14426PU-N | STK33 (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP54076PU-N | STK33 (N-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
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|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306910 | STK33 |
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| Recombinant protein of human serine/threonine kinase 33 (STK33) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403089 | STK33 overexpression lysate |
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0.1 mg /
€295.00
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|
| OriGene Technologies, Inc. |