STK4 antibody
Principal name
STK4 antibody
Alternative names for STK4 antibody
Serine/threonine-protein kinase 4, MST1, STE20-like kinase MST1, MST-1, STE20-like kinase MST1, Mammalian STE20-like protein kinase 1, Serine/threonine-protein kinase Krs-2
Ncbi ID
9606, NM_006282, NM_006282.2, NP_006273.1, NP_067395.1, NP_006273, NM_021420
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of STK4 antibody
- Kit Component:
- - KN316887G1, Stk4 gRNA vector 1 in pCas-Guide vector
- KN316887G2, Stk4 gRNA vector 2 in pCas-Guide vector
- KN316887D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to STK4 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. [antibody concentration 5ug/ml]
Immunofluorescence of monoclonal antibody to STK4 on HeLa cell. [antibody concentration 10 ug/ml]
STK4 monoclonal antibody (M01), clone 1D7-8A10 Western Blot analysis of STK4 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged STK4 is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to STK4 on HeLa cell. [antibody concentration 25 ug/ml]
Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and STK4 HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-STK4 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
STK4 monoclonal antibody (M02), clone 4F4 Western Blot analysis of STK4 expression in HeLa ( Cat # L013V1 ).
STK4 monoclonal antibody (M02), clone 4F4. Western Blot analysis of STK4 expression in NIH/3T3 ( Cat # L018V1 ).
STK4 monoclonal antibody (M02), clone 4F4. Western Blot analysis of STK4 expression in Raw 264.7 ( Cat # L024V1 ).
STK4 monoclonal antibody (M02), clone 4F4. Western Blot analysis of STK4 expression in PC-12 ( Cat # L012V1 ).
Detection limit for recombinant GST tagged STK4 is approximately 0.1ng/ml as a capture antibody.
STK4 polyclonal antibody (A01), Lot # 060707JCS1. Western Blot analysis of STK4 expression in NIH/3T3.
STK4 polyclonal antibody (A01), Lot # 060707JCS1 Western Blot analysis of STK4 expression in Jurkat ( Cat # L017V1 ).
STK4 polyclonal antibody (A01), Lot # 060707JCS1. Western Blot analysis of STK4 expression in Raw 264.7.
STK4 polyclonal antibody (A01), Lot # 060707JCS1. Western Blot analysis of STK4 expression in PC-12.
Western blot analysis of STK4 in 15 µgs of Jurkat cell lysate using STK4 polyclonal antibody ( Cat # PAB0373 ) at 1 µg/ml.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the STK4 polyclonal antibody ( Cat # PAB2295 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of STK4 polyclonal antibody ( Cat # PAB2295 ) in HL-60 cell lysate. MST1 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
(LEFT)Western blot analysis of anti-MST1 Pab in HL60 cell lysate. MST1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of STK4 (arrow) using MST1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the STK4 gene (Lane 2) (Origene Technologies).
Western blot analysis of STK4 expression in Jurkat cell lysate.
Representative Standard Curve
Representative Standard Curve
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308695 diluted at 1:2000.
Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. STK4 antibody. TA308695 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MST1(TA308695) antibody at 1:500 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using STK4(TA308695) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
STK4 antibody immunoprecipitates STK4 protein in IP experiments. IP samples: HepG2 whole cell extract. A. 50 ug HepG2 whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of STK4 protein by 4 ug STK4 antibody (TA308695)
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310831 | STK4 |
||||
| Recombinant protein of human serine/threonine kinase 4 (STK4) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
