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swi6 antibody

Principal name

swi6 antibody

Alternative names for swi6 antibody

Chromatin-associated protein swi6

SwissProt ID

P40381 (Schpo)

Gene ID

2541633 (swi6)

Ncbi ID

NP_593449, 4896, NP_593449.1

Available reactivities

Ye (Yeast), Hu (Human), Ms (Mouse)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP)

Background of swi6 antibody

The chromatin structure of Fission yeast centromeres is regulated epigenetically. In the central core domain, the histone H3 variant Cnp1(CENP-A) plays a key role. In the flanking heterochromatin domain, histones are kept underacetylated by the histone deacetylases (HDACs) Clr3, Clr6 and Sir2, and methylated by Clr4 methyltransferase (HMTase) to create a specific binding site for the Swi6 protein. Swi6 then directly mediates cohesin binding to the centromeric heterochromatin. Recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. Involved in the repression of the silent mating-type loci MAT2 and MAT3. May compact MAT2/3 into a heterochromatin-like conformation which represses the transcription of these silent cassettes. Swi6 interacts with histone H3 methylated at Lys-9 and with the cohesin subunit psc3. Swi6 associates with Swi4 to activate HO and many other late G1-specific transcripts in budding yeast. In contrast, histone H3 methylated at Lys9 and its interacting protein Swi6/HP1, which define heterochromatin, coated extended domains associated with a variety of repeat elements and small islands corresponding to meiotic genes. Contains two chromo domains.

Western Blot: Swi6 Antibody [NB600-932] - Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kDa corresponding to S.pombe Swi6 protein. ~35μg of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 μm nitrocellulose. The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye?800 labeled Goat-a-Rabbit IgG [H&L] (611-132- 122) diluted 1:5,000 for 45 min.

NB600-932

Western blot analysis is shown using swi6 polyclonal antibody ( Cat # PAB11378 ) to detect endogenous protein present in S.pombe lysate ( arrowhead ).Comparison to a molecular weight marker ( not shown ) indicatesa band of ~43 kDa corresponding to S.pombe Swi6 protein.Approx. ~35?g of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 µm nitrocellulose.The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 h.

PAB11378

Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kDa corresponding to S.pombe Swi6 protein. ~35µg of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 µm nitrocellulose. The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye(TM)800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

AP09279PU-N

Schematic model of S. pombe centromere cluster in interphase cells. The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.

AP09279PU-N

The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.

GTX48740

Western blot analysis is shown using GeneTex's Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kDa corresponding to S.pombe Swi6 protein. ~35?g of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 ?m nitrocellulose. The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-anti-Rabbit IgG [H&L] diluted 1:5,000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

GTX48740

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
AP09279PU-N	swi6 (314-328) antibody
	Rabbit	IgG	Aff - Purified	Ye	E, WB	0.1 mg / €515.00
						OriGene Technologies GmbH
+1 additional image

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