Home » Target » Synaptotagmin-1 antibody

Synaptotagmin-1 antibody

Principal name

Synaptotagmin-1 antibody

Alternative names for Synaptotagmin-1 antibody

SYT1, SVP65, SYT, Synaptotagmin1, Synaptotagmin I, SytI, p65

SwissProt ID

P21579 (Human), P21707 (Rat), P46096 (Mouse), P47191 (Chick), P48018 (Bovin)

Gene ID

6857 (SYT1), 25716 (Syt1), 20979

Ncbi ID

9606, NP_001129277, NP_001028852, 10116, NP_001129277.1, NP_033332.1, NP_005630, NP_001129278, NP_001028852.2, NM_001033680, NM_001135805, NM_001135806

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Bov (Bovine), Can (Canine), Mky (Monkey), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot)

Background of Synaptotagmin-1 antibody

Synaptotagmin I (Syt1), located on human chromosome 12, is a member of synaptotagmin protein family. Syt1 is an integral membrane protein of synaptic vesicles and it is involved in both endocytosis and exocytosis (1). Syt1 is known to directly interact with the SNARE complex; it contains two C2 domains (C2A and C2B) that are homologous in sequence and 3-D in structure, but have distinct biochemical and biological properties (2). The C2A domain binds Ca2+ thus allowing Syt1 to act as a calcium sensor in the Ca2+-dependent release of neurotransmitter vesicles whereas the C2B domain binds inositol high polyphosphates (2). In vertebrates, Syt1 is required for a type of exocytosis (fast, synchronous release) (1). The intestinal epithelial Syt1 has been shown to play an important role in cAMP-stimulated endocytosis of apical membrane Na(+)-H(+) exchanger (3). Syt1 can also function as a protein receptor for Botulinum neurotoxins (BoNT/B and BoNT/G) (4).
The expression of Syt1 is negatively regulated by Akt (protein kinase B) and inhibition of Akt leads to an increase in the expression of Syt1 genes (5). A high expression of Syt1 is found in the brains of patients with refractory epilepsy (6). Deficiency of Syt1 in mice resulted in their death with 24 hours. These mice also exhibited motor dysfunction, reduction in body weight and growth (7).
Syt1 is expressed throughout the brain, but it is mainly expressed in the cytoplasm and cytomembrane of neurons.
Sty1 is approximately a 50.6kDa protein (422 amino acids).

General readings

Han C., et al. (2009) J. Immunol. 182:2986-2996.

Immunoperoxidase of monoclonal antibody to SYT1 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 1.5 ug/ml]

H00006857-M05

Detection limit for recombinant GST tagged SYT1 is approximately 1ng/ml as a capture antibody.

H00006857-M05

Western blot (WB) analysis of SYT antibody (Cat.-No.: AP06736PU-N) in extracts from HepG2 cells.

AP06736PU-N

Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using Synaptotagmin I/II antibody AP08066PU (Red).

Figure 1. Western blot analysis of extract from Hela cells using Synaptotagmin I/II Antibody AP08066PU (Lane 1 and 2).

SYT1 monoclonal antibody (M01), clone 3A5. Western Blot analysis of SYT1 expression in Jurkat ( Cat # L017V1 ).

H00006857-M01

Detection limit for recombinant GST tagged SYT1 is approximately 3ng/ml as a capture antibody.

H00006857-M01

AP16929PU-N SYT1 antibody staining of paraffin embedded Human Brain at 3.8 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

AP16929PU-N

Antibody (1µg/ml) staining of Human Hippocampus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AP16929PU-N

Western blot of rat cortex lysate showing specific labeling of the ~65k Syt1 protein. Using Syt1 polyclonal antibody ( Cat # PAB9639 ).

PAB9639

Syt1 (phospho S309) polyclonal antibody ( Cat # PAB9648 ) and PSD-95 polyclonal antibody ( Cat # PAB9623 ) double-label immunostaining of 14 DIV cortical neurons.

PAB9648

Western blot of rat cortex lysate showing specific immunolabeling of the ~60k~62k Syt1 S309 phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Syt1 (phospho S309) polyclonal antibody ( Cat # PAB9648 ). The immunolabeling is completely eliminated by treatment with l-Ptase.

PAB9648

Syt1 (phospho T202) polyclonal antibody ( Cat # PAB9667 ) and PSD-95 polyclonal antibody ( Cat # PAB9623 ) double-label immunostaining of 14 DIV cortical neurons.

PAB9667

Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k Syt1 phosphorylated at Thr202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Syt1 (phospho T202) polyclonal antibody ( Cat # PAB9667 ). The immunolabeling is completely eliminated by treatment with l-Ptase.

PAB9667

Figure 2. Synaptotagmin pThr202 and PSD95 Double-Label Immunostaining of 14 DIV Cortical Neurons.

AP08747PU-N

Figure 1. Western blot of rat cortex lysate showing specific immunolabeling of the ~60k-62k synaptotagmin phosphorylated at Thr202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Thr202 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

AP08747PU-N

Figure 2. Synaptotagmin pSer309 and PSD95 Double-Label Immunostaining of 14 DIV Cortical Neurons.

AP08748PU-N

Figure 1. Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k Synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

AP08748PU-N

Immunofluorescence staining of methanol-fixed Hela cells using Synaptotagmin Antibody (Phospho-Ser309) Cat.-No AP09491PU

Immunofluorescence staining of methanol-fixed HeLa cells using Synaptotagmin Antibody

Western Blot analysis of extracts from mouse brain and rat brain tissue using Synaptotagmin antibody

Immunohistochemistry Analysis: AP20200PU-N Synaptotagmin antibody staining of Paraffin-Embedded Human breast carcinoma tissue.

AP20200PU-N

Western blot (WB) analysis of Synaptotagmin antibody (Cat.-No.: AP20200PU-N) in extracts from HeLa cells.

AP20200PU-N

Immunohistochemistry Analysis: AP20201PU-N Synaptotagmin antibody staining of Paraffin-Embedded Human breast carcinoma tissue.

AP20201PU-N

Western blot (WB) analysis of Synaptotagmin antibody (Cat.-No.: AP20201PU-N) in extracts from 293 cells Forskolin 40nM 30' or 293 cells Sorbitol 0.4M 30'.

AP20201PU-N

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Synaptotagmin (Phospho-Ser309) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 293 cells treated with Sobital 0.4M 30', using Synaptotagmin (Phospho-Ser309) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Synaptotagmin (Phospho-Thr202) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 293 cells treated with Forskolin 40nM 30', using Synaptotagmin (Phospho-Thr202) Antibody. The lane on the left is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Synaptotagmin (Ab-309) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 293 cells, treated with Forskolin 40nM 30', using Synaptotagmin (Ab-309) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Synaptotagmin (Ab-202) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells, using Synaptotagmin (Ab-202) Antibody. The lane on the right is treated with the synthesized peptide.

Mouse outer plexiform; . Sample Type:Â outer mouse plexiform layer. Red:Â Primary. Blue:Â DAPI. Primary Dilution:Â 1:200. Secondary Antibody:Â Goat anti-Rabbit AF568 IgG(H+L). Secondary Dilution:Â 1:200. Image Submitted by:Â David Zenisek. Yale UniversityÂ .; Syt1 antibody - middle region (ARP59447_P050) in Mouse outer plexiform cells using Immunohistochemistry

ARP59447_P050

Mouse retina; . Sample Type: complete mouse retina sections. Red: Primary. Blue: DAPI. Primary Dilution: 1:200. Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L). Secondary Dilution: 1:200. Image Submitted by: David Zenisek. Yale UniversityÂ .; Syt1 antibody - middle region (ARP59447_P050) in Mouse retina cells using Immunohistochemistry

ARP59447_P050

Mouse cortex brain; . Lanes: Lane1: 10ug mouse cortex brain lysate. Lane2: 25ug mouse cortex brain lysate. Lane3: 40ug mouse cortex brain lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:2000. Gene Name: Syt1. Submitted by: Anonymous.; Syt1 antibody - middle region (ARP59447_P050) in Mouse cortex brain cells using Western Blot

ARP59447_P050

Mouse Spleen; Syt1 antibody - middle region (ARP59447_P050) validated by WB using Mouse Spleen lysate at 1.0ug/ml.; Syt1 antibody - middle region (ARP59447_P050) in Mouse Spleen cells using Western Blot

ARP59447_P050

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

AM31018PU-N

Detection limit for recombinant GST tagged SYT1 is approximately 1ng/ml as a capture antibody.

AM31018PU-N

Recombinant human Synaptotagmin-1, 136-382aa, His-tagged

Western blot showing a single Synaptotagmin bond running at about 65 kDa

AP31821PU-N

Western blot analysis using SYT1 antibody Cat.-No AM06290SU-N against truncated Trx-SYT1 recombinant protein (Lane 1).

AM06290SU-N

Western blot analysis Synaptotagmin1 expression in rat brain extracts (1) or PC12 (2) using AM26493AF-N.

AM26493AF-N

Western blot analysis of Synaptotagmin 1/2 expression in rat brain extracts (1) and PC12 (2) using AM26691AF-N.

AM26691AF-N

Immunoprecipitation of Synaptotagmin 1/2 from rat brain extracts with mouse IgG2a (1) or AM26691AF-N (2) After immunoprecipitated with the antibody, immunocomplex was resolved on SDSPAGE and immunoblotted with AM26691AF-N.

AM26691AF-N

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Synaptotagmin (phospho-Thr202) antibody AP55688PU-N (left)or the same antibody preincubated with blocking peptide (right).

Western blot analysis of extracts from colo cells (Lane 2), using Synaptotagmin (Phospho-Thr202) Antibody AP55688PU-N. The lane on the left is treated with antigen-specific peptide.

Immunohistochemical analysis of Synaptotagmin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of Synaptotagmin expression in HEK293T (A); Jurakt (B) whole cell lysates.

TP308938

TP327105

TP327252

Western blot analysis of Synaptotagmin expression in HEK293T (A); mouse brain (B) whole cell lysates.

Immunohistochemical analysis of Synaptotagmin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Synaptotagmin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot of rat cortex lysate showing specific labeling of the ~65k synaptotagmin protein.

TA309247

Western blot analysis of Synaptotagmin expression in Forskolin treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325927

Western blot analysis of Synaptotagmin expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325928

Western blot analysis of various cell lines, using SYT1 antibody.

TA326884

Immunofluorescence analysis of HeLa cell using SYT1 antibody. Blue: DAPI for nuclear staining.

TA326884

Western blot analysis of rat brain membranes: 1. Anti-Synaptotagmin-I antibody, (1:200). 2. Anti-Synaptotagmin-I antibody, preincubated with the control peptide antigen.

TA328891

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
AM26691AF-N	Synaptotagmin 1 + 2 antibody
	Mouse	IgG2a	1D12	Azide Free	Rt	ICC/IF, IP, WB	0.1 mg / €400.00
							OriGene Technologies GmbH
+1 additional image
AP16929PU-N	Synaptotagmin-1 antibody
	Goat			Aff - Purified	Bov, Can, Hu, Ms, Rt	E, P, WB	0.1 mg / €380.00
							OriGene Technologies GmbH
+1 additional image	OriGene recommends this product for Western blotting.
TA309247	Synaptotagmin-1 antibody
	Rabbit Anti-synaptotagmin 1 Antibody
	Rabbit	IgG		Aff - Purified	Bov, Chk, Hu, Ms, non-Human Primates, Rt	WB	0.1 ml / €405.00
							OriGene Technologies, Inc.
TA325927	Synaptotagmin-1 antibody
	Rabbit Polyclonal Synaptotagmin Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA325928	Synaptotagmin-1 antibody
	Rabbit Polyclonal Synaptotagmin Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA326884	Synaptotagmin-1 antibody
	SYT1 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA328891	Synaptotagmin-1 antibody
	Rabbit Polyclonal Anti-Synaptotagmin-I
	Rabbit			Purified	Ms, Rt	WB	0.2 ml / €585.00
							OriGene Technologies, Inc.
AM06290SU-N	Synaptotagmin-1 antibody
	Mouse	IgG1	8G11B10	Ascites	Hu	WB	0.1 ml / €380.00
							OriGene Technologies GmbH
AM26493AF-N	Synaptotagmin-1 (N-term) antibody
	Mouse	IgG2a	3F10	Azide Free	Rt	WB	0.1 mg / €400.00
							OriGene Technologies GmbH
AM31018PU-N	Synaptotagmin-1 antibody
	Mouse	IgG2a	1A6	Purified	Hu	E, P, WB	50 µg / €475.00
							OriGene Technologies GmbH
+1 additional image
AP06736PU-N	Synaptotagmin-1 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
AP09491PU-N	Synaptotagmin-1 pSer309 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	ICC/IF	0.1 mg / €300.00
							OriGene Technologies GmbH
AP09491PU-S	Synaptotagmin-1 pSer309 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	ICC/IF	50 µg / €230.00
							OriGene Technologies GmbH
AP09507PU-N	Synaptotagmin-1 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	ICC/IF, WB	0.1 mg / €300.00
							OriGene Technologies GmbH
+1 additional image
AP09507PU-S	Synaptotagmin-1 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	ICC/IF, WB	50 µg / €230.00
							OriGene Technologies GmbH
+1 additional image
AP20200PU-N	Synaptotagmin-1 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+1 additional image
AP20201PU-N	Synaptotagmin-1 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+2 additional images
AP31821PU-N	Synaptotagmin-1 antibody
	Chicken	Ig		Aff - Purified	Hu, Ms	ICC/IF, P, WB	0.4 mg / €550.00
							OriGene Technologies GmbH
AP55688PU-N	Synaptotagmin-1 pThr202 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €300.00
							OriGene Technologies GmbH
+1 additional image
AP55688PU-S	Synaptotagmin-1 pThr202 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	50 µg / €230.00
							OriGene Technologies GmbH
+1 additional image

Antibodies (see all»)

Kits (see all»)

Antigens

Lysates (see all»)

Tissue Slides (see all»)

Other life-science products