Synaptotagmin-3 antibody
Principal name
Synaptotagmin-3 antibody
Alternative names for Synaptotagmin-3 antibody
Synaptotagmin 3, SYT3, SYT-3, Synaptotagmin III, SytIII
Ncbi ID
NP_115674, NP_001153800, NP_001153801, NP_001107588.1, NP_001153800.1, NM_016663, NM_001114116, NM_019122, NM_032298, NM_001160328, NM_001160329
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of Synaptotagmin-3 antibody
Synaptotagmins constitute a family of membranetrafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins.
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2 (1, 2).
Synaptotagmin-3 may play a role in insulin release in pancreatic βcells, and may also play a role in CXCR4 mediated T-cell migration processes (3).
General readings
Jones JM. et al. (1995) Mamm Genome. 6:212–213.Li C. et al. (1995) Nature. 375:594–599.
mouse, rat, and human brain; SYT3 antibody - N-terminal region (ARP50058_P050) validated by WB using mouse, rat, and human brain lysates at 1ug/ml.; SYT3 antibody - N-terminal region (ARP50058_P050) in mouse, rat, and human brain cells using Western Blot
Human 293T; WB Suggested Anti-SYT3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate; SYT3 antibody - N-terminal region (ARP50058_P050) in Human 293T cells using Western Blot
Immunofluorescence against SUR1 WT (left) and null mutant (right) mouse striatum and stained with N289/16 (green) and commercial antibody against Tyrosine Hydroxylase (blue). Images courtesy of Paul Witkovsky, Jyoti Patel and Margaret Rice, New York University Medical Center.
Adult rat brain membrane immunoblot
Immunofluorescence against SUR1 WT (left) and null mutant (right) mouse striatum and stained with N289/16 (green) and commercial antibody against Tyrosine Hydroxylase (blue). Images courtesy of Paul Witkovsky, Jyoti Patel and Margaret Rice, New York University Medical Center.
Adult rat brain membrane immunoblot
SYT3 antibody - N-terminal region validated by WB using mouse, rat, and human brain lysates at 1 ug/ml.
WB Suggested Anti-SYT3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (SYT3 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA349442 | Synaptotagmin-3 antibody |
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| Rabbit Polyclonal Anti-SYT3 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| 73-269 | Synaptotagmin-3 antibody |
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 |
Mouse | IgG1 | N278/19 | Supernatant | Hu, Ms, Rt | ICC/IF, WB |
5 ml /
€490.00
|
| Antibodies Incorporated | |||||||
 +1 additional image |
|
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| 75-269 | Synaptotagmin-3 antibody |
||||||
 |
Mouse | IgG1 | N278/19 | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€570.00
|
| Antibodies Incorporated | |||||||
| +1 additional image |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305963 | Synaptotagmin-3 |
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| Recombinant protein of human synaptotagmin III (SYT3) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP328969 | Synaptotagmin-3 (transcript variant 2) |
||||
| Recombinant protein of human synaptotagmin III (SYT3), transcript variant 2. | |||||
 |
Human | > 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP328970 | Synaptotagmin-3 (transcript variant 3) |
||||
| Recombinant protein of human synaptotagmin III (SYT3), transcript variant 3. | |||||
 |
Human | > 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209162L2V | Lenti ORF particles, Syt3 (GFP-tagged) - Mouse synaptotagmin III (Syt3), transcript variant 1, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,320.00
|
|
| OriGene Technologies, Inc. | ||
| MR216575L2V | Lenti ORF particles, Syt3 (GFP-tagged) - Mouse synaptotagmin III (Syt3), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,320.00
|
|
| OriGene Technologies, Inc. | ||
| MR209162L1V | Lenti ORF particles, Syt3 (Myc-DDK-tagged) - Mouse synaptotagmin III (Syt3), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,180.00
|
|
| OriGene Technologies, Inc. | ||
| MR216575L1V | Lenti ORF particles, Syt3 (Myc-DDK-tagged) - Mouse synaptotagmin III (Syt3), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,180.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR204869L2V | Lenti ORF particles, Syt3 (GFP-tagged ORF) - Rat synaptotagmin III (Syt3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,310.00
|
|
| OriGene Technologies, Inc. | ||
| RR204869L1V | Lenti ORF particles, Syt3 (Myc-DDK-tagged ORF) - Rat synaptotagmin III (Syt3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,180.00
|
|
| OriGene Technologies, Inc. | ||