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Synaptotagmin-5 antibody

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Principal name

Synaptotagmin-5 antibody

Alternative names for Synaptotagmin-5 antibody

Synaptotagmin 5, SYT5, SYT-5, Synaptotagmin V, SytV

SwissProt ID

O00445 (Human), P47861 (Rat), Q9R0N5 (Mouse)

Gene ID

6861 (SYT5), 53420 (Syt5), 54309

Ncbi ID

NP_003171.2, NP_003171, NP_058604.1, NM_019350

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Goat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of Synaptotagmin-5 antibody

Synaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse.

General readings

Craxton M.A., Olsen A., et al. (1997), Genomics 42:165-169

ARP59484_P050 - Synaptotagmin-5
Mouse retina; . Sample Type: complete mouse retina sections. Red: Primary. Blue: DAPI. Primary. Dilution:.  1:200. Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L). Secondary. Dilution:.  1:200. Image Submitted by: David Zenisek. Yale University .; SYT5 antibody - middle region (ARP59484_P050) in Mouse retina cells using Immunohistochemistry
ARP59484_P050 - Synaptotagmin-5
Human Fetal Kidney; WB Suggested Anti-SYT5 Antibody Titration: 1 ug/ml. Positive Control: Fetal kidney lysate; SYT5 antibody - middle region (ARP59484_P050) in Human Fetal Kidney cells using Western Blot
AR51211PU-N - Synaptotagmin-5
TP308528 - Synaptotagmin-5
75-306 - Synaptotagmin-5
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N276A/15 (left) and N86/8 (right)
73-306 - Synaptotagmin-5
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N276A/15 (left) and N86/8 (right)
TA311461 - Synaptotagmin-5
(1ug/ml) staining of Olfactory bulb lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA322890 - Synaptotagmin-5
Predicted band size: 43 kDa . Positive control: Human thigh malignant fibrous histiocytoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 8 hours)
TA322890 - Synaptotagmin-5
Predicted cell location: Cytoplasm . Positive control: Human brain tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using SYT5 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification: x200)
TA322891 - Synaptotagmin-5
Predicted band size: 43 kDa . Positive control: Human thigh malignant fibrous histiocytoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 8 hours)
TA322891 - Synaptotagmin-5
Predicted cell location: Cytoplasm . Positive control: Human brain tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using SYT5 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x200)
TA342628 - Synaptotagmin-5
WB Suggested Anti-SYT5 Antibody Titration: 1 ug/ml; Positive Control: Fetal kidney lysate
TA342628 - Synaptotagmin-5
Sample Type: complete mouse retina sections; Red: Primary; Blue: DAPI; Primary; Dilution: 1:200; Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L); Secondary; Dilution: 1:200; Image Submitted by: David Zenisek; Yale University

8 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Synaptotagmin-5 (80-93) antibody
  Goat Anti-SYT5/ Synaptotagmin-5 (aa 80-93) Antibody  
(1ug/ml) staining of Olfactory bulb lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

Synaptotagmin-5 antibody
  Anti-SYT5 Rabbit Polyclonal Antibody  
Predicted band size: 43 kDa . Positive control: Human thigh malignant fibrous histiocytoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 8 hours) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

Synaptotagmin-5 antibody
  Anti-SYT5 Rabbit Polyclonal Antibody  
Predicted band size: 43 kDa . Positive control: Human thigh malignant fibrous histiocytoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 8 hours) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

Synaptotagmin-5 antibody
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N276A/15 (left) and N86/8 (right) Mouse IgG1 N276A/15 Supernatant Hu, Ms, Rt C, ICC/IF, WB
5 ml / €490.00
  Antibodies Incorporated
 

NeuroMab

Synaptotagmin-5 antibody
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N276A/15 (left) and N86/8 (right) Mouse IgG1 N276A/15 Purified Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €570.00
  Antibodies Incorporated
 

NeuroMab

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Synaptotagmin-5
  Recombinant protein of human synaptotagmin V (SYT5)  
Synaptotagmin-5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Syt5 (GFP-tagged ORF) - Rat synaptotagmin V (Syt5), 200 uL, >10^7 TU/mL
Lenti ORF particles, Syt5 (GFP-tagged ORF) - Rat synaptotagmin V (Syt5), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Syt5 (Myc-DDK-tagged ORF) - Rat synaptotagmin V (Syt5), 200 uL, >10^7 TU/mL
Lenti ORF particles, Syt5 (Myc-DDK-tagged ORF) - Rat synaptotagmin V (Syt5), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

8 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (5)
  2. Protein | Growth Factor (1)
  3. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (3)
  2. Monoclonal (2)
Target
  1. Synaptotagmin-5 (6)
Property
  1. 80-93 (1)
Reactivity
  1. Human (Hu) (5)
  2. Mouse (Ms) (5)
  3. Rat (Rt) (4)
  4. Bovine (Bov) (1)
  5. Canine (Can) (1)
  6. Porcine (Por) (1)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Frozen sections (C) (2)
  3. Enzyme Immunoassay (E) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  5. Paraffin Sections (P) (2)
Presentation
  1. Purified (3)
  2. Aff - Purified (1)
  3. Supernatant (1)
Host
  1. Mouse (2)
  2. Rabbit (2)
  3. Goat (1)
Images
  1. 1 image (6)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. IHC (2)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (6)
  2. Antibodies Incorporated (2)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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