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Synaptotagmin-7 antibody

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Principal name

Synaptotagmin-7 antibody

Alternative names for Synaptotagmin-7 antibody

Synaptotagmin 7, SYT7, SYT-7, Synaptotagmin VII, SytVII, IPCA-7, PCANAP7, Prostate cancer-associated protein 7

SwissProt ID

O43581 (Human), Q9R0N7 (Mouse)

Gene ID

9066 (SYT7), 54525 (Syt7)

Ncbi ID

9606, NP_004191.2, NP_061271.1, NM_173068, NM_018801, NM_173067, NM_004200

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of Synaptotagmin-7 antibody

Synaptotagmins constitute a family of membrane trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family.There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. 
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2.
Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.

75-265 - Synaptotagmin-7
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N275/14 (left) and N86/8 (right).
75-265 - Synaptotagmin-7
Adult rat brain membrane immunoblot
73-265 - Synaptotagmin-7
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N275/14 (left) and N86/8 (right).
73-265 - Synaptotagmin-7
Adult rat brain membrane immunoblot
TA324206 - Synaptotagmin-7
Predicted band size: 46kDa. Positive control: Lncap and SKOV3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Lncap cell lysate Lane2: SKOV3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute)
TA324206 - Synaptotagmin-7
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using SYT7 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200)
TA324207 - Synaptotagmin-7
Predicted band size: 46kDa. Positive control: SKOV3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 5 minutes)
TA324207 - Synaptotagmin-7
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human liver cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using SYT7 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200)
TA326504 - Synaptotagmin-7
WB of rat brain lysate showing detection of ~65 kDa Synaptotagmin 7 protein using Anti-Synaptotagmin 7. Lane 1: MW ladder. Lane 2: Anti-Synaptotagmin 7 (1:100). Lane 3: Anti-Synaptotagmin 7 (1:250). Lane 4: Anti-Synaptotagmin 7 (1:500). Lane 5: Anti-Synaptotagmin 7 (1:1000). Load: 20 µg RB lysate per lane. Block: 5% milk + TBST 1hr at RT. Primary antibody: Mouse Anti-Synaptotagmin 7 monoclonal antibody incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:50 for 60 min at RT. Colour Development: TMB solution for 30 min at RT. Predicted/Observed size: ~ 65 kDa. Other band(s) (faint): ~25kDa, ~37kDa, ~100kDa, ~150kDa.

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Synaptotagmin-7 antibody
  Anti-SYT7 rabbit polyclonal antibody  
Predicted band size: 46kDa. Positive control: Lncap and SKOV3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Lncap cell lysate Lane2: SKOV3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

Synaptotagmin-7 antibody
  Anti-SYT7 rabbit polyclonal antibody  
Predicted band size: 46kDa. Positive control: SKOV3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

Synaptotagmin-7 (150-239, Cytopl. Dom.) antibody
  Mouse Monoclonal Anti-Synaptotagmin-7 Antibody  
WB of rat brain lysate showing detection of ~65 kDa Synaptotagmin 7 protein using Anti-Synaptotagmin 7. Lane 1: MW ladder. Lane 2: Anti-Synaptotagmin 7 (1:100). Lane 3: Anti-Synaptotagmin 7 (1:250). Lane 4: Anti-Synaptotagmin 7 (1:500). Lane 5: Anti-Synaptotagmin 7 (1:1000). Load: 20 µg RB lysate per lane. Block: 5% milk + TBST 1hr at RT. Primary antibody: Mouse Anti-Synaptotagmin 7 monoclonal antibody incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:50 for 60 min at RT. Colour Development: TMB solution for 30 min at RT. Predicted/Observed size: ~ 65 kDa. Other band(s) (faint): ~25kDa, ~37kDa, ~100kDa, ~150kDa. Mouse IgG2b S275-14 Purified Ms, Rt ICC/IF, P, WB
0.1 mg / €410.00
  OriGene Technologies, Inc.

Synaptotagmin-7 antibody
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N275/14 (left) and N86/8 (right). Mouse IgG2b N275/14 Supernatant Ms, Rt C, ICC/IF, WB
5 ml / €490.00
  Antibodies Incorporated
+1 additional image

NeuroMab

Synaptotagmin-7 antibody
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N275/14 (left) and N86/8 (right). Mouse IgG2b N275/14 Purified Ms, Rt C, ICC/IF, WB
0.1 ml / €570.00
  Antibodies Incorporated
+1 additional image

NeuroMab

5 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (5)
Clonality
  1. Monoclonal (3)
  2. Polyclonal (2)
Target
  1. Synaptotagmin-7 (5)
Property
  1. 150-239, Cytopl. Dom. (1)
Reactivity
  1. Mouse (Ms) (5)
  2. Rat (Rt) (5)
  3. Human (Hu) (2)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
  3. Paraffin Sections (P) (3)
  4. Frozen sections (C) (2)
  5. Enzyme Immunoassay (E) (2)
Presentation
  1. Purified (4)
  2. Supernatant (1)
Host
  1. Mouse (3)
  2. Rabbit (2)
Images
  1. 1 image (5)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. IHC (2)
Manufacturer
  1. OriGene Technologies (3)
  2. Antibodies Incorporated (2)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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