TBCA antibody
Principal name
TBCA antibody
Alternative names for TBCA antibody
Tubulin-specific chaperone A, Tubulin-folding cofactor A, TCP1-chaperonin cofactor A
SwissProt ID
O75347 (Human)
Gene ID
6902 (TBCA)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB)
Background of TBCA antibody
- Kit Component:
- - KN202032G1, TBCA gRNA vector 1 in pCas-Guide vector
- KN202032G2, TBCA gRNA vector 2 in pCas-Guide vector
- KN202032D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Nolasco S., et al. (2005). FEBS Lett. 579(17):3515-24.
Western Blot analysis of TBCA expression in transfected 293T cell line by TBCA monoclonal antibody (M03), clone 1D2.Lane 1: TBCA transfected lysate(12.9 KDa).Lane 2: Non-transfected lysate.
Recombinant human TBCA, 1-108 aa: 15% SDS-PAGE (3 µg)
Cell lysates of LNCaP(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TBCA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis: Cell lysates of LNCaP (20 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TBCA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
TBCA Antibody (Center) (Cat. #AP54173PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TBCA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
TBCA Antibody (Center) (Cat. #AP54173PU-N) western blot analysis in mouse kidney tissue lysates (35µg/lane).This demonstrates the TBCA antibody detected the TBCA protein (arrow).
TBCA Antibody (N-term) (Cat. #AP54174PU-N) western blot analysis in WiDr cell line lysates (35µg/lane).This demonstrates the TBCA antibody detected the TBCA protein (arrow).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM09084PU-N | TBCA antibody |
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 |
Mouse | IgG2b | AT1A5 | Purified | Hu | E, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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| AM09084PU-S | TBCA antibody |
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|
Mouse | IgG2b | AT1A5 | Purified | Hu | E, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP54173PU-N | TBCA (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP54174PU-N | TBCA (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302032 | TBCA |
||||
| Recombinant protein of human tubulin folding cofactor A (TBCA) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||