TCF25 antibody
Principal name
TCF25 antibody
Alternative names for TCF25 antibody
Transcription factor 25, KIAA1049, NULP1, Nuclear localized protein 1
Ncbi ID
9606, NP_055787, NP_055787.1, NM_014972, NP_001032966.1, NM_001037877, NM_001037878, NM_025804
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of TCF25 antibody
- Kit Component:
- - KN317324G1, Tcf25 gRNA vector 1 in pCas-Guide vector
- KN317324G2, Tcf25 gRNA vector 2 in pCas-Guide vector
- KN317324D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Steen,H. (2008) Biochem. Biophys. Res. Commun. 366 (2), 432-437
Western Blot analysis of TCF25 expression in transfected 293T cell line (H00022980-T01) by TCF25 MaxPab polyclonal antibody.Lane 1: NULP1 transfected lysate(74.36 KDa).Lane 2: Non-transfected lysate.
Human Jurkat; WB Suggested Anti-TCF25 Antibody Titration: 5.0ug/ml. Positive Control: Jurkat cell lysate; TCF25 antibody - N-terminal region (ARP47624_T100) in Human Jurkat cells using Western Blot
Transfected 293T; WB Suggested Anti-TCF25 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; TCF25 antibody - N-terminal region (ARP47623_P050) in Transfected 293T cells using Western Blot
Sample (30 ug of whole cell lysate). A: Hela. 7.5% SDS PAGE. TA308555 diluted at 1:500.
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308555 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using NULP1(TA308555) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NULP1(TA308555) antibody at 1:200 dilution.
TCF25 Antibody (N-term) (Cat. #TA324616) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TCF25 antibody detected the TCF25 protein (arrow).
IF image of A549 cell stained with TCF25 Antibody (N-term)(Cat#TA324616).A549 cells were incubated with TCF25 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).TCF25 immunoreactivity is localized to Cytoplasm significantly.
WB Suggested Anti-TCF25 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Transfected 293T
WB Suggested Anti-TCF25 Antibody Titration: 5.0 ug/ml; Positive Control: Jurkat cell lysate
Host: Rabbit; Target Name: NULP1; Sample Tissue: ACHN whole cell lysates; Antibody Dilution: 1.0 ug/ml
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308555 | TCF25 antibody |
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| Rabbit Polyclonal antibody to NULP1 (transcription factor 25 (basic helix-loop-helix)) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA324616 | TCF25 antibody |
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| Rabbit polyclonal TCF25 Antibody (N-term) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | ICC/IF, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP55326SU-N | TCF25 antibody |
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 |
Rabbit | Serum | Hu | C, E, WB |
0.2 ml /
€430.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304078 | TCF25 |
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| Recombinant protein of human transcription factor 25 (basic helix-loop-helix) (TCF25) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY414869 | TCF25 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |