TCF4 antibody
Principal name
TCF4 antibody
Alternative names for TCF4 antibody
Transcription factor 4, TCF-4, ITF2, ITF-2, SEF2, SEF-2, Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2
Ncbi ID
NP_003190, 9606, NP_001077431.1, NP_001139746.1, NP_001230165, NP_001077431, NP_001077436.1, BC014293, NM_013685, NM_001083967, NM_053369, NM_003199, NM_001083962
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Electrophoretic Mobility Shift Assay (EMSA), Frozen Sections (C)
Background of TCF4 antibody
- Kit Component:
- - KN317326G1, Tcf4 gRNA vector 1 in pCas-Guide vector
- KN317326G2, Tcf4 gRNA vector 2 in pCas-Guide vector
- KN317326D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Sepp, M. et al. (2012) Human Molecular Genetics. 21:2873–2888
de Pontual L. et al. (2009) Hum. Mutat. 30:669–676.
Human HT1080; Host: Rabbit. Target Name: TCF4. Sample Tissue: HT1080 Whole Cell lysates. Antibody Dilution: 1.0ug/ml.
Human Placenta; WB Suggested Anti-TCF4 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Placenta; TCF4 antibody - N-terminal region (P100775_P050) in Human Placenta cells using Western Blot
TCF4 monoclonal antibody (M02), clone 1F6 Western Blot analysis of TCF4 expression in A-549 ( Cat # L025V1 ).
Immunofluorescence of monoclonal antibody to TCF4 on HeLa cell. [antibody concentration 10 ug/ml]
TCF4 monoclonal antibody (M03), clone 1G4 Western Blot analysis of TCF4 expression in K-562 ( Cat # L009V1 ).
Detection limit for recombinant GST tagged TCF4 is approximately 1ng/ml as a capture antibody.
Figure 1. Western blot analysis using AM05271PU-N antibody at 1 µg/ml on Cos-1 cell lysate control (Lane 1) and Cos-1 cells transfected with human TCF-4 (Lane 2).
Figure 1. Western blot analysis using AM05272PU-N antibody at 4 µg/ml on Cos-1 cell lysate control (Lane 1) and Cos-1 cells transfected with human TCF-3 (Lane 2).
Immunofluorescence of monoclonal antibody to TCF4 on HeLa cell. [antibody concentration 10 ug/ml]
TCF4 monoclonal antibody (M01), clone 3E10 Western Blot analysis of TCF4 expression in A-549 ( Cat # L025V1 ).
Immunofluorescence of monoclonal antibody to TCF4 on HeLa cell. [antibody concentration 10 ug/ml]
TCF4 monoclonal antibody (M04), clone 3H4 Western Blot analysis of TCF4 expression in A-549 ( Cat # L025V1 ).
human neuroblastoma; TCF4 antibody - N-terminal region (P100776_P050) validated by WB using 1. SHSY-5Y lysate (15ug) at 1:100.. sH-SY5Y cell lysate;no + transfected hTCF4;yes (human neuroblastoma cells); TCF4 antibody - N-terminal region (P100776_P050) in human neuroblastoma cells using Western Blot
HepG2; WB Suggested Anti-TCF4 Antibody . Titration: 0.2-1 ug/ml. Positive Control: HepG2; TCF4 antibody - N-terminal region (P100776_P050) in HepG2 cells using Western Blot
Western blot analysis of TCF4 expression in COS-1 tissue control ( lane 1 ) and COS-1 cells transfected with human TCF4 ( lane 2 ) probed with TCF4 monoclonal antibody, clone 6H5-3 at 1 µg/ml.
AP32964PU-N (0.5µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical staining of human testis shows distinct nuclear positivity in seminiferus duct cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemical analysis of TCF4/12 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of TCF4/12 expression in K562 (A); A549 (B) whole cell lysates.
Western blot analysis of extracts from HUVEC cells, using TCF4/12 antibody.The lane on the right is treated with the synthesized peptide.
Predicted band size: 71 kDa . Positive control: K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of extracts from COS7 cells (line 1) treated with EGF (200ng/ml, 30mins) and 293 cells (line 2) treated with H2O2 (100µM, 15mins), using CDK5 (Phospho-Tyr15) antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cell using TCF4 antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-TCF4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Placenta
Host: Rabbit; Target Name: TCF4; Sample Tissue: HT1080 Whole Cell lysates; Antibody Dilution: 1.0ug/ml
WB Suggested Anti-TCF4 Antibody; Titration: 0.2-1 ug/ml; Positive Control: HepG2
TCF4 antibody - N-terminal region validated by WB using 1. SHSY-5Y lysate (15ug) at 1:100.; sH-SY5Y cell lysate;no + transfected hTCF4;yes (human neuroblastoma cells)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP32964PU-N | TCF4 antibody |
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 |
Goat | Aff - Purified | Can, Hu, Ms | WB |
0.1 mg /
€380.00
|
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| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
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| TA314598 | TCF4 antibody |
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| Rabbit polyclonal anti-TCF4/12 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA326945 | TCF4 antibody |
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| Rabbit anti-TCF4 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| RA18032-100 | TCF4 antibody |
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 |
Rabbit | Aff - Purified | Chk, Hu, Ms, Rt | IP, WB |
0.1 ml /
€420.00
|
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| Neuromics Antibodies | |||||||
| RA18033-100 | TCF4 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, IP, WB |
0.1 ml /
€420.00
|
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| Neuromics Antibodies | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN217718 | TCF4 - human gene knockout kit via CRISPR |
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 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP317718 | TCF4 (transcript variant 2) |
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| Recombinant protein of human transcription factor 4 (TCF4), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP324345 | TCF4 (transcript variant 1) |
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| Recombinant protein of human transcription factor 4 (TCF4), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP760702 | TCF4 (N-term HIS tag, transcript variant 2) |
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| Purified recombinant protein of Human transcription factor 4 (TCF4), transcript variant 2, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208151L2V | Lenti ORF particles, Tcf4 (GFP-tagged) - Mouse transcription factor 4 (cDNA clone MGC:13998 IMAGE:4014231), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR209915L2V | Lenti ORF particles, Tcf4 (GFP-tagged) - Mouse transcription factor 4 (Tcf4), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,430.00
|
|
| OriGene Technologies, Inc. | ||
| MR208151L1V | Lenti ORF particles, Tcf4 (Myc-DDK-tagged) - Mouse transcription factor 4 (cDNA clone MGC:13998 IMAGE:4014231), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| MR209915L1V | Lenti ORF particles, Tcf4 (Myc-DDK-tagged) - Mouse transcription factor 4 (Tcf4), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,280.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR212726L2V | Lenti ORF particles, Tcf4 (GFP-tagged ORF) - Rat transcription factor 4 (Tcf4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,310.00
|
|
| OriGene Technologies, Inc. | ||
| RR212726L1V | Lenti ORF particles, Tcf4 (Myc-DDK-tagged ORF) - Rat transcription factor 4 (Tcf4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,170.00
|
|
| OriGene Technologies, Inc. | ||
