TDG antibody
Principal name
TDG antibody
Alternative names for TDG antibody
G/T mismatch-specific thymine DNA glycosylase
Gene ID
6996 (TDG)
Ncbi ID
9606, NP_003202.3, NP_003202, AAH37557, AAH37557.1, NM_003211
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E)
Background of TDG antibody
In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. G/T mismatch-specific Thymine DNA Glycosylase (TDG) is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymine. TDG also corrects a subset of G/U mispairs inefficiently removed by the more abundant uracil glycosylases. Retinoic acid receptors interact physically and functionally with TDG, enhancing the ability of the retinoid X receptor and the retinoid X receptor/retinoid acid receptor complex to bind to their response elements. TDG interacts with, and is covalently modified by, the ubiquitin-like proteins SUMO-1 and SUMO-2/3, resulting in a reduction of the DNA substrate and AP site binding affinity of TDG. This sumoylation is associated with a significant increase in enzymatic turnover in reactions with a G/U substrate and the loss of G/T processing activity.
General readings
Neddermann, P ., et al. (1996) J Biol Chem 271(22): 12767-74
Sard, L., et al. (1997) Genomics 44(2): 222-6
Gallinari, P., et al. (1996) Nature 383(6602): 735-8
Western blot (WB) analysis of TDG antibody (Cat.-No.: AP06727PU-N) at 1/500 dilution
Lane1:Hela whole cell lysate
Lane2:Mouse lung tissue lysate
Lane1:Hela whole cell lysate
Lane2:Mouse lung tissue lysate
Immunofluorescence of purified MaxPab antibody to TDG on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of TDG expression in transfected 293T cell line (H00006996-T01) by TDG MaxPab polyclonal antibody.Lane 1: TDG transfected lysate(45.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of TDG expression in transfected 293T cell line (H00006996-T01) by TDG MaxPab polyclonal antibody.Lane 1: TDG transfected lysate(46.00 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of TDG antibody (C-term) (AP17780PU-N) in K562 cell line lysates (35ug/lane). TDG (arrow) was detected using the purified Pab.
Western blot analysis of TDG antibody (N-term) (AP17781PU-N) in mouse lung tissue lysates (35ug/lane). TDG (arrow) was detected using the purified Pab.
The cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TDG antibody (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescent analysis of Thymine DNA Glycosylase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Thymine DNA Glycosylase expression in HeLa (A); mouse lung (B); rat lung (C) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808511)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808511)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808511)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TDG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808511)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TDG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808511)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808511)(1:150)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TDG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-TDG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TDG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TDG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TDG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TDG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TDG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TDG (RC207113, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TDG.(1:2000)
Western blot analysis of extracts from Jurkat cells, using TDG antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts of various cell lines, using TDG antibody.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA808511 | TDG antibody |
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| TDG mouse monoclonal antibody, clone OTI3A12 (formerly 3A12) | |||||||
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Mouse | IgG1 | OTI3A12 | Purified | Hu | P |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody!
|
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| TA808610 | TDG antibody |
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| TDG mouse monoclonal antibody, clone OTI3C11 (formerly 3C11) | |||||||
 |
Mouse | IgG1 | OTI3C11 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +13 additional images |
Highly validated antibody!
|
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| TA314606 | TDG antibody |
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| Rabbit polyclonal anti-TDG antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA332890 | TDG antibody |
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| Rabbit anti-TDG Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| AM50036PU-N | TDG antibody |
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 |
Mouse | IgG1 | AT2F7 | Purified | Hu | E, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
| AM50036PU-S | TDG antibody |
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|
Mouse | IgG1 | AT2F7 | Purified | Hu | E, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| AP06727PU-N | TDG antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
| AP17780PU-N | TDG (C-term) antibody |
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 |
Rabbit | Hu | WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
| AP17781PU-N | TDG (N-term) antibody |
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 |
Rabbit | Hu, Ms | WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
