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TDGF1 / CRIPTO antibody

Principal name

TDGF1 / CRIPTO antibody

Alternative names for TDGF1 / CRIPTO antibody

Cripto-1 growth factor, CRGF, Teratocarcinoma-derived growth factor 1, Epidermal growth factor-like cripto protein CR1

SwissProt ID

P13385 (Human), P51865 (Mouse)

Gene ID

6997 (TDGF1)

Ncbi ID

9606, NP_003203, NP_001167607.1, NP_003203.1, NM_003212, NM_001174136

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Prim (Primate)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TDGF1 / CRIPTO antibody

Cripto-1 (teratocarcinoma-derived growth factor-1) is the origil member of EGF-cripto-1-FRL-1-cryptic (CFC) family of vertebrate sigling molecules which is essential for embryonic development and it implicates in carcinogenesis also. Cripto1 functions as a co-receptor for NODAL (TGF-beta member) and is essential for mesoderm as well as endoderm formation and anterior-posterior and left-right axis establishment. After executing key functions during embryonic development, it dramatically disappears in adult tissues (except stem cells). Cripto-1 modulate sigling of TGF-beta family members (NODAL, GDF-1/-3, Activin, TGF-beta1 etc), and activate c-src/MAPK/AKT pathway in a Glypican-1/GRP78-dependent manner, and cross-talks with erbB4, Wnt/beta-catenin, NOTCH, Caveolin-1, and Apelin/putative receptor protein related to angiotensin-type I receptor pathways. During embryonic development, Cripto-1 together with Nodal controls cell migration and EMT, whereas, posttally, it regulates the branching morphogenesis of mammary gland. Moreover, in adult life it gets re -expressed in several type of tumors wherein it promotes cell proliferation, migration, invasion, EMT, and angiogenesis.

General readings

1. Parisi S, D\1Andrea D, Lago CT, Adamson ED, Persico MG, Minchiotti G Nodal-dependent Cripto signaling promotes cardiomyogenesis and redirects the neural fate of embryonic stem cells. J Cell Biol. 2003 Oct 27;163(2):303-14
PMID: 14581455

Formalin-fixed and paraffin-embedded human cancer tissue reacted with TDGF1 polyclonal antibody ( Cat # PAB3871 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma

PAB3871

The TDGF1 polyclonal antibody ( Cat # PAB3871 ) is used in Western blot to detect TDGF1 in A-375 cell lysate.

PAB3871

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

AP11913PU-N

The anti-TDGF1 N-term (AP11913PU-N) is used in Western blot to detect TDGF1 in A375 cell lysate.

AP11913PU-N

IHC Image for Cat#AP15504PU-S / AP15504PU-N

Western blot analysis of Tdgf1 in 20 µg of MDA-MB-21 lysate with Tdgf1 polyclonal antibody ( Cat# PAB12003 ).

PAB12003

Western blot analysis of TDGF1 in 20 µg of MDA-MB-21 lysate with TDGF1 polyclonal antibody ( Cat# PAB12004 ).

PAB12004

Western blot using TDGF1 polyclonal antibody ( Cat # PAB9995 ) shows detection of endogenous and transfected TDGF1 from mouse and human sources.The TDGF1 band appears above the 17.8 kDa marker.Endogenous detection is shown using mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells.EpH4 CR-1 is a mouse mammary epithelial cell line stably expressing mouse TDGF1.NTERA cells are human embryonal carcinoma cells that, when overgrown, differentiate and lose TDGF1 expression.COS-7 cells transfected with human TDGF1 expression vector were used as a positive control and EpH4 WT cells were used as a negative control.A non-specific band at 45 kDa may be present in some preparations.The primary antibody was used at a 1 : 500 dilution.Personal communication, C. Bianco, NCI, Bethesda, MD.

PAB9995

WB using Anti-Cripto-1 shows detection of endogenous and transfected Cripto-1 from mouse or human. Endogenous detection using mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells. EpH4 CR-1 is a mouse mammary epithelial cell line stably expressing Cripto-1. NTERA cells are human embryonal carcinoma cells that, when overgrown, differentiate and lose Cripto-1 expression. COS7 cells transfected with human Cripto-1 expression vector were used as a positive control and EpH4 WT cells were used as a negative control. Dilution: 1:500 .

Immunohistochemistry: AP20147PU-N CRIPTO Antibody staining of Paraffin Embedded Human Placenta at 3.8 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

AP20147PU-N

Human Jurkat; WB Suggested Anti-TDGF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; TDGF1 antibody - middle region (ARP45440_P050) in Human Jurkat cells using Western Blot

ARP45440_P050

Western blot analysis using TDGF1 mAb against HEK293 (1) and TDGF1(AA: 31-188)-hIgGFc transfected HEK293 (2) cell lysate.

AM06616SU-N

Red: Control Antigen (100ng);Purple: Antigen (10ng);Green: Antigen (50ng);Blue: Antigen (100ng);

AM06616SU-N

Human TDGF1 (Teratocarcinoma-derived growth factor 1) recombinant protein

PRO-50022-0050

Human TDGF1 (Teratocarcinoma-derived growth factor 1) recombinant protein

PRO-50022-0025

Human TDGF1 (Teratocarcinoma-derived growth factor 1) recombinant protein

PRO-50022-0010

ELISA: Cripto1 Antibody

TA590152

Western Blot: Cripto1 Antibody - Analysis of Cripto in MDA-MB231 lysates.

TA301467

Immunocytochemistry/Immunofluorescence: Cripto1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue) and tubulin was stained with alpha tubulin (red).

TA301467

Flow Cytometry: Staining of NTERA-2 cells using Cripto1 Antibody at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary.

TA301467

Western blot analysis of TDGF1 in 293 cell lysate with TDGF1 antibody at 1 ug/mL.

TA319716

Immunofluorescence of TDGF1 in 293 cells with TDGF1 antibody at 20 ug/mL.

TA319716

Western Blot: Cripto1 Antibody [TA336263] - Analysis of Cripto in Dicer knockout mouse embryonic stem cell lysate.

TA336263

Immunocytochemistry/Immunofluorescence: Cripto1 Antibody [TA336263] - Cripto1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

TA336263

Flow Cytometry: Cripto1 Antibody [TA336263] - Staining of NTERA-2 cells using TA336263 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody.

TA336263

Human Colon Carcinoma stained with anti-Cripto-1 antibody.

TA353763

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (TDGF1 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200)

TA351104

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA301467	TDGF1 / CRIPTO antibody
	Rabbit Polyclonal Antibody against Cripto - Embryonic Stem Cell Marker
	Rabbit	IgG		Purified	Hu	WB	0.1 ml / €415.00
							OriGene Technologies, Inc.
+2 additional images
TA319168	TDGF1 / CRIPTO antibody
	Rabbit polyclonal Cripto-1 TDGF1 antibody
	Rabbit	IgG		Aff - Purified	Hu, Ms	E, WB	0.1 mg / €515.00
							OriGene Technologies, Inc.
TA319716	TDGF1 / CRIPTO antibody
	Rabbit Polyclonal TDGF1 Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	ICC/IF, WB	0.1 mg / €415.00
							OriGene Technologies, Inc.
+1 additional image
TA336263	TDGF1 / CRIPTO antibody
	Rabbit Polyclonal CRIPTO Antibody
	Rabbit			Purified	Hu, Ms	F, ICC/IF, WB	0.1 ml / €450.00
							OriGene Technologies, Inc.
+2 additional images
TA351104	TDGF1 / CRIPTO antibody
	Rabbit Polyclonal Anti-TDGF1 Antibody
	Rabbit	IgG		Purified	Hu	E, P	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA590152	TDGF1 / CRIPTO antibody
	Rabbit Polyclonal Cripto1 Antibody
	Rabbit	IgG		Purified	Hu	E	0.1 mg / €409.00
							OriGene Technologies, Inc.
AM06616SU-N	TDGF1 / CRIPTO antibody
	Mouse	IgG1	3A6	Ascites	Hu	E, WB	0.1 ml / €410.00
							OriGene Technologies GmbH
+1 additional image
AP11913PU-N	TDGF1 / CRIPTO (N-term) antibody
	Rabbit	Ig		Purified	Hu	P, WB	0.4 ml / €415.00
							OriGene Technologies GmbH
+1 additional image
AP20147PU-N	TDGF1 / CRIPTO antibody
	Goat			Aff - Purified	Bov, Can, Hu, Ms, Rt	E, P	0.1 mg / €380.00
							OriGene Technologies GmbH
RA25091-100	TDGF1 / CRIPTO antibody
	Rabbit	IgG		Aff - Purified	Bov, Can, Hu, Ms, Prim, Rt	F, ICC/IF, WB	0.1 ml / €380.00
							Neuromics Antibodies
+2 additional images