TEC antibody
Principal name
TEC antibody
Alternative names for TEC antibody
PSCTK4, Tyrosine-protein kinase Tec
Ncbi ID
9606, NM_003215, NP_003206, NP_003206.2, AAI01712.1, 5874, NP_001106931.1, NM_001113461
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of TEC antibody
- Kit Component:
- - KN317387G1, Tec gRNA vector 1 in pCas-Guide vector
- KN317387G2, Tec gRNA vector 2 in pCas-Guide vector
- KN317387D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Formalin-fixed and paraffin-embedded human cancer tissue reacted with TEC polyclonal antibody ( Cat # PAB3439 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of TEC polyclonal antibody ( Cat # PAB3439 ) in mouse liver tissue lysate. TEC ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Human THP-1; WB Suggested Anti-TEC Antibody Titration: 0.2-1 ug/ml. Positive Control: THP-1 cell lysate; TEC antibody - middle region (ARP51209_P050) in Human THP-1 cells using Western Blot
TEC Antibody (N-term) (Cat. AP54214PU-N) western blot analysis in ZR-75-1 cell line lysates (35 µg/lane). This demonstrates the TEC antibody detected the TEC protein (arrow).
Western blot analysis using TEC mouse mAb against TEC (aa90-240)-hIgGFc transfected HEK293 cell lysate (1).
Western blot analysis of anti-TEC Pab (Cat. #TA324978) in mouse liver tissue lysate. TEC (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
TEC Antibody (F190) (Cat. #TA324978) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TEC antibody detected the TEC protein (arrow).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
IF image of Hela cell stained with TEC Antibody (Center)(Cat#TA324978).Hela cells were incubated with TEC primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.
IF image of Hela cell stained with TEC Antibody (Center)(Cat#TA324978).Hela cells were incubated with TEC primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.
WB Suggested Anti-TEC Antibody Titration: 0.2-1 ug/ml; Positive Control: THP-1 cell lysate
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324978 | TEC (Center) antibody |
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| Rabbit polyclonal TEC Antibody (Center) | |||||||
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Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| AM06363SU-N | TEC antibody |
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Mouse | IgG1 | 3A5 | Ascites | Hu | WB |
0.1 ml /
€340.00
|
| OriGene Technologies GmbH | |||||||
| AP54214PU-N | TEC (N-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760589 | TEC (full length, N-term HIS tag) |
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| Purified recombinant protein of Human tec protein tyrosine kinase (TEC), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY418830 | TEC overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209373L2V | Lenti ORF particles, Tec (GFP-tagged) - Mouse tec protein tyrosine kinase (Tec), transcript variant 3, 200 uL, >10^7 TU/mL |
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200 µl /
€1,340.00
|
|
| OriGene Technologies, Inc. | ||
| MR209373L1V | Lenti ORF particles, Tec (Myc-DDK-tagged) - Mouse tec protein tyrosine kinase (Tec), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,200.00
|
|
| OriGene Technologies, Inc. | ||
