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TERT antibody

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Principal name

TERT antibody

Alternative names for TERT antibody

Telomerase reverse transcriptase, HEST2, EST2, TCS1, TRT, Telomerase catalytic subunit, Telomerase-associated protein 2

SwissProt ID

O14746 (Human), O70372 (Mouse), Q27ID4 (Bovin), Q673L6 (Rat), Q6A548 (Canfa)

Gene ID

7015 (TERT), 21752 (Tert), 301965

Ncbi ID

9606, NP_001180305.1, NP_001180305, NP_033380.1, NM_198253, NM_009354, NM_053423, NM_001193376

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Dot blot (Dot), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)

Background of TERT antibody

Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication that is active in progenitor as well as cancer cells, and stays ictive or show very low activity in normal somatic cells. TERT is the catalytic component of the holoenzyme complex (TERT, DKC1, WDR79/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) that implicates in telomeres elongation by acting as a reverse transcriptase adding simple sequence repeats to chromosome ends by copying a template sequence within R component of the enzyme. For executing its versatile functions, TERT can interact with HSP90A , PTGES3, HSPA1A, RAN , XPO1, PTPN11, NCL, SMARCA4, MCRS1, PIF1, PML and GNL3L. TERT modulates Wnt sigling and plays important roles in aging and anti-apoptosis. Telomerase activation has been implicated in cell immortalization/ carcinogenesis and defects in TERT are associated with susceptibilty to aplastic anemia, corory artery disease (CAD), dyskeratosis congenita autosomal domint type 2 (DKCA2), pulmory fibrosis, and/or bone marrow failure, telomere-related, type 1 (PFBMFT1), dyskeratosis congenita autosomal recessive type 4 (DKCB4), susceptibility to pulmory fibrosis idiopathic (IPF).

PAB2490 - TERT
Western blot analysis of TERT polyclonal antibody ( Cat # PAB2490 ) in Jurkat cell line lysates ( 35 µg/lane ) . TERT ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
PAB4934 - TERT
Dot blot analysis of TERT (phospho S1125) polyclonal antibody ( Cat # PAB4934 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.
AP12956PU-N - TERT
Dot blot analysis of anti-Phospho-TERT pSer1125 Antibody (Cat.#AP12956PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
AP13029PU-N - TERT
Dot blot analysis of anti-Phospho-TERT-pY707 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
MAB2379 - TERT
Western blot analysis of human TERT using TERT monoclonal antibody, clone 2D8 ( Cat # MAB2379 ). Sample : MJ90 whole cell lysate.
PAB12002 - TERT
Western blot analysis of TERT in HeLa nuclear extracts with TERT polyclonal antibody ( Cat# PAB12002 ).
A1182-1 - TERT
Immunofluorescence analysis of HuvEc cells, using Telomerase (Phospho-Ser227) Antibody. The picture on the right is treated with the synthesized peptide.
A1183-1 - TERT
Western blot analysis of extracts from COLO205 cells, using Telomerase (Phospho-Ser824) Antibody. The lane on the right is treated with the synthesized peptide.
B1182-1 - TERT
Immunofluorescence analysis of HuvEc cells, using Telomerase (Ab-227) Antibody. The picture on the right is treated with the synthesized peptide.
B1183-1 - TERT
Western blot analysis of extracts from Jurkat cells, using Telomerase (Ab-824) Antibody. The lane on the right is treated with the synthesized peptide.
AP31849PU-N - TERT
Staining of Skeletal Muscle lysate (35µg protein in RIPA buffer) using TERT Antibody Cat.-No AP31849PU-N at 0.5 µg/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
CPA3022-100ul - TERT
Western blot analysis of Telomerase expression in Jurkat (A); Raw264.7 (B); PC12 (C) whole cell lysates.
AP33476PU-N - TERT
Fluorescent confocal image of SY5Y cells stained with TERT antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated TERT primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). TERT immunoreactivity is localized to the nucleus of SY5Y cells.
AP33476PU-N - TERT
Fluorescent confocal image of HeLa cell stained with TERT Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TERT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TERT immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.

AP33476PU-N - TERT
TERT Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

AP33476PU-N - TERT
Western blot of anti-TERT Antibody in Jurkat cell line lysates (35 ug/lane). TERT(arrow) was detected using the purified antibody.
AP33476PU-N - TERT
Formalin-Fixed, Paraffin-Embedded Human Skin tissue stained with Anti-TERT / Telomerase antibody Cat.-No AP33476PU-N at 1/100 dilution after heat-induced antigen retrieval.

TA301587 - TERT
Western Blot analysis of human TERT
TA301588 - TERT
Western Blot analysis of human TERT 1:500 dilution on MJ90 cells
TA301588 - TERT
Normal pancreas showing moderate staining of exocrine cells and a subset of islets of Langerhans
TA301588 - TERT
Immunocytochemistry/Immunofluorescence: Telomerase reverse transcriptase Antibody (2C4) - Detection of Tert (Green) in HepG2 cells. Nuclei (Blue) are counterstained with Hoechst 33258.
TA301588 - TERT
Flow Cytometry: Telomerase reverse transcriptase Antibody (2C4) - Staining of NTERA-2 cells at a 1:200 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.
TA312511 - TERT
Immunofluorescence analysis of HUVEC cells, using Telomerase antibody.The picture on the right is treated with the synthesized peptide.
TA321951 - TERT
Predicted cell location: Cytoplasm. Positive control: Human gastric cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TERT antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification: x200)
TA324097 - TERT
Predicted cell location: Cytoplasm . Positive control: Human tonsil and esophagus cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using TERT antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200)
TA324097 - TERT
Predicted cell location: Cytoplasm . Positive control: Human tonsil and esophagus cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using TERT antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA324098 - TERT
Predicted cell location: Cytoplasm or Nucleus . Positive control: Human thyroid and ovarian cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using TERT antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x 200)
TA324098 - TERT
Predicted cell location: Cytoplasm or Nucleus . Positive control: Human thyroid and ovarian cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using TERT antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA324535 - TERT
Western blot analysis of anti-TERT Antibody (Center) (RB14069) in Jurkat cell line lysates (35ug/lane). TERT(arrow) was detected using the purified Pab.
TA324535 - TERT
IF image of Hela cell stained with TERT Antibody (Center)(Cat#TA324535).Hela cells were incubated with TERT primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . TERT immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.
TA324535 - TERT
IF image of SY5Y cells stained with TERT (Center) antibody. SY5Y cells were incubated with TA324535 TERT (Center) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . TERT immunoreactivity is localized to the nucleus of SY5Y cells.
TA324535 - TERT
TERT Antibody (Center) (Cat. #TA324535) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA336564 - TERT
Western Blot: Telomerase reverse transcriptase Antibody [TA336564] - Detection of Telomerase reverse transcriptase in HeLa nuclear extracts using TA336564.
TA336564 - TERT
Immunocytochemistry/Immunofluorescence: Telomerase reverse transcriptase Antibody [TA336564] - The Telomerase reverse transcriptase antibody was tested in V6.5 mouse embryonic stem cells (cat# NBP1-41162) against Dylight 488. Actin and nuclei were counte
TA348015 - TERT
Western blot analysis of TERT expression in Jurkat cell extracts
TA348918 - TERT
Western blot analysis of Telomerase (Phospho-Ser824) expression in Jurkat cell extracts
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TERT (627-656) antibody
Fluorescent confocal image of SY5Y cells stained with TERT antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated TERT primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). TERT immunoreactivity is localized to the nucleus of SY5Y cells. Rabbit Purified Hu F, ICC/IF, P, WB
0.2 ml / €425.00
  OriGene Technologies GmbH
+4 additional images

Highly validated antibody!

TERT (597-611) antibody
Staining of Skeletal Muscle lysate (35µg protein in RIPA buffer) using TERT Antibody Cat.-No AP31849PU-N at 0.5 µg/ml. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €380.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Western blotting.

TERT antibody
  Mouse Monoclonal Antibody against Telomerase reverse transcriptase (2D8) - Embryonic Stem Cell Marker  
Western Blot analysis of human TERT Mouse IgM 2D8 Purified Hu WB
0.1 ml / €415.00
  OriGene Technologies, Inc.

TERT antibody
  Mouse Monoclonal Antibody against Telomerase reverse transcriptase (2C4) - Embryonic Stem Cell Marker  
Western Blot analysis of human TERT 1:500 dilution on MJ90 cells Mouse IgM 2C4 Purified Hu F, ICC/IF, P, WB
0.1 ml / €465.00
  OriGene Technologies, Inc.
+3 additional images

TERT antibody
  Rabbit polyclonal Telomerase antibody  
Immunofluorescence analysis of HUVEC cells, using Telomerase antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF
0.1 ml / €335.00
  OriGene Technologies, Inc.

TERT antibody
  Anti-TERT Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human gastric cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TERT antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

TERT antibody
  Anti-TERT Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm . Positive control: Human tonsil and esophagus cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using TERT antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

TERT antibody
  Anti-TERT Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm or Nucleus . Positive control: Human thyroid and ovarian cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using TERT antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

TERT (Center) antibody
  Rabbit polyclonal TERT Antibody (Center)  
Western blot analysis of anti-TERT Antibody (Center) (RB14069) in Jurkat cell line lysates (35ug/lane). TERT(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+3 additional images

TERT antibody
  Rabbit Polyclonal TERT Antibody  
Western Blot: Telomerase reverse transcriptase Antibody [TA336564] - Detection of Telomerase reverse transcriptase in HeLa nuclear extracts using TA336564. Rabbit Purified Hu, Ms ICC/IF, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

TERT antibody
  Rabbit Polyclonal Anti-TERT Antibody  
Western blot analysis of TERT expression in Jurkat cell extracts Rabbit IgG Purified Hu WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

TERT antibody
  Rabbit Polyclonal Anti-Telomerase (Phospho-Ser824) Antibody  
Western blot analysis of Telomerase (Phospho-Ser824) expression in Jurkat cell extracts Rabbit IgG Purified Hu WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

TERT pSer1125 antibody
Dot blot analysis of anti-Phospho-TERT pSer1125 Antibody (Cat.#AP12956PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €415.00
  OriGene Technologies GmbH

TERT pTyr707 antibody
Dot blot analysis of anti-Phospho-TERT-pY707 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €415.00
  OriGene Technologies GmbH

CRISPR

Catalog No. Species Donor Vector  

TERT - human gene knockout kit via CRISPR
TERT - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TERT - human gene knockout kit via CRISPR
TERT - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

TERT - human gene knockout kit via CRISPR
TERT - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TERT - human gene knockout kit via CRISPR
TERT - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

TERT overexpression lysate
TERT overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, TERT (mGFP-tagged) - Human telomerase reverse transcriptase (TERT), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, TERT (mGFP-tagged) - Human telomerase reverse transcriptase (TERT), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €2,390.00
  OriGene Technologies, Inc.
 

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25 products - FILTER YOUR RESULTS!

Available Filters

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Target
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Property
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Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
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