TESK2 antibody
Principal name
TESK2 antibody
Alternative names for TESK2 antibody
Testicular protein kinase 2, Dual specificity testis-specific protein kinase 2
Ncbi ID
9606, NP_009101, NP_009101.2, NM_007170, NM_133396, NP_666263.3
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of TESK2 antibody
- Kit Component:
- - KN317420G1, Tesk2 gRNA vector 1 in pCas-Guide vector
- KN317420G2, Tesk2 gRNA vector 2 in pCas-Guide vector
- KN317420D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml]
Western blot analysis of TESK2 over-expressed 293 cell line, cotransfected with TESK2 Validated Chimera RNAi ( Cat # H00010420-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TESK2 monoclonal antibody (M01) clone 1A8 (Cat # H00010420-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M01), clone 1A8.Lane 1: TESK2 transfected lysate(56.137 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml]
TESK2 monoclonal antibody (M02), clone 6C7 Western Blot analysis of TESK2 expression in IMR-32 ( Cat # L008V1 ).
Detection limit for recombinant GST tagged TESK2 is approximately 0.1ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml]
Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M04), clone 5H4.Lane 1: TESK2 transfected lysate(60.3 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged TESK2 is approximately 0.1ng/ml as a capture antibody.
Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M05), clone 5D1.Lane 1: TESK2 transfected lysate(60.3 KDa).Lane 2: Non-transfected lysate.
Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.7 ug/ml]
Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M08), clone 5G1.Lane 1: TESK2 transfected lysate(60.3 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged TESK2 is approximately 0.1ng/ml as a capture antibody.
Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M11), clone 5C3.Lane 1: TESK2 transfected lysate(60.3 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged TESK2 is approximately 1ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human testis tissue reacted with TESK2 polyclonal antibody ( Cat # PAB3502 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of TESK2 polyclonal antibody ( Cat # PAB3502 ) in HL-60 cell line lysates ( 35 µg/lane ) . TESK2 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Formalin-fixed and paraffin-embedded human testis tissue reacted with TESK2 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-TESK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). TESK2 (arrow) was detected using the purified Pab (1:60 dilution).
Western Blot analysis of TESK2 expression in transfected 293T cell line (H00010420-T02) by TESK2 MaxPab polyclonal antibody.Lane 1: TESK2 transfected lysate(60.30 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of TESK2 expression in transfected 293T cell line (H00010420-T02) by TESK2 MaxPab polyclonal antibody.Lane 1: TESK2 transfected lysate(60.30 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of TESK2 expression in Raji (A); HepG2 (B); NIH3T3 (C) whole cell lysates.
Sample (50 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA308623 diluted at 1:1000.
Sample(30 ug whole cell lysate). A:Raji . 7.5% SDS PAGE. TA308623 diluted at 1:500
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TESK2(TA308623) antibody at 1:200 dilution.
Western blot analysis of extracts from HepG2 cells and HeLa cells, using TESK2 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-TESK2 Antibody; Titration: 1.0 ug/ml; Positive Control: MCF7 Whole Cell
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308623 | TESK2 antibody |
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| Rabbit Polyclonal antibody to TESK2 (testis-specific kinase 2) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA312773 | TESK2 antibody |
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| Rabbit polyclonal anti-TESK2 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP14646PU-N | TESK2 (Center) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP324117 | TESK2 |
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| Recombinant protein of human testis-specific kinase 2 (TESK2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416143 | TESK2 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR200698L2V | Lenti ORF particles, Tesk2 (GFP-tagged ORF) - Rat testis-specific kinase 2 (Tesk2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,280.00
|
|
| OriGene Technologies, Inc. | ||
| RR200698L1V | Lenti ORF particles, Tesk2 (Myc-DDK-tagged ORF) - Rat testis-specific kinase 2 (Tesk2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,150.00
|
|
| OriGene Technologies, Inc. | ||
