TFAP4 antibody
Principal name
TFAP4 antibody
Alternative names for TFAP4 antibody
Transcription factor AP-4, Activating enhancer-binding protein 4
SwissProt ID
Q01664 (Human)
Gene ID
7023 (TFAP4)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of TFAP4 antibody
- Kit Component:
- - KN203773G1, TFAP4 gRNA vector 1 in pCas-Guide vector
- KN203773G2, TFAP4 gRNA vector 2 in pCas-Guide vector
- KN203773D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Petersenn,S., et al., (1998) Mol. Endocrinol. 12 (2), 233-247
Immunoperoxidase of monoclonal antibody to TFAP4 on formalin-fixed paraffin-embedded human uterine cervix. [antibody concentration 1 ug/ml]
Immunofluorescence of monoclonal antibody to TFAP4 on HeLa cell. [antibody concentration 10 ug/ml]
TFAP4 monoclonal antibody (M01), clone 6B1 Western Blot analysis of TFAP4 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged TFAP4 is approximately 0.1ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to TFAP4 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1 ug/ml]
Western blot analysis of TFAP4 over-expressed 293 cell line, cotransfected with TFAP4 Validated Chimera RNAi ( Cat # H00007023-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TFAP4 monoclonal antibody (M03), clone 7A10 (Cat # H00007023-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
TFAP4 monoclonal antibody (M03), clone 7A10 Western Blot analysis of TFAP4 expression in Hela NE ( Cat # L013V3 ).
Western Blot analysis of TFAP4 expression in transfected 293T cell line by TFAP4 monoclonal antibody (M03), clone 7A10.Lane 1: TFAP4 transfected lysate(38.7 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged TFAP4 is approximately 0.1ng/ml as a capture antibody.
TFAP4 polyclonal antibody (A01), Lot # 051005JC01 Western Blot analysis of TFAP4 expression in Hela NE ( Cat # L013V3 ).
Human Muscle; TFAP4 antibody - C-terminal region (ARP33435_T100) in Human Muscle cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-TFAP4 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate. TFAP4 is supported by BioGPS gene expression data to be expressed in HepG2.; TFAP4 antibody - C-terminal region (ARP33435_T100) in Human HepG2 cells using Western Blot
Human Brain; TFAP4 antibody - C-terminal region (ARP33436_T100) in Human Brain cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-TFAP4 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; TFAP4 antibody - C-terminal region (ARP33436_T100) in Human Jurkat cells using Western Blot
Immunoperoxidase of monoclonal antibody to TFAP4 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml]
Western Blot analysis of TFAP4 expression in transfected 293T cell line by TFAP4 monoclonal antibody (M05), clone 8G6.Lane 1: TFAP4 transfected lysate(38.87 KDa).Lane 2: Non-transfected lysate.
TFAP4 monoclonal antibody (M05), clone 8G6. Western Blot analysis of TFAP4 expression in Jurkat(Cat # L017V1 ).
Detection limit for recombinant GST tagged TFAP4 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to TFAP4 on HeLa cell. [antibody concentration 10 ug/ml]
TFAP4 monoclonal antibody (M02), clone 7C5. Western Blot analysis of TFAP4 expression in human colon.
TFAP4 monoclonal antibody (M02), clone 7C5 Western Blot analysis of TFAP4 expression in Hela NE ( Cat # L013V3 ).
TFAP4 monoclonal antibody (M02), clone 7C5. Western Blot analysis of TFAP4 expression in human colon.
TFAP4 monoclonal antibody (M02), clone 7C5 Western Blot analysis of TFAP4 expression in Hela NE ( Cat # L013V3 ).
TFAP4 MaxPab rabbit polyclonal antibody. Western Blot analysis of TFAP4 expression in mouse kidney.
Western Blot analysis of TFAP4 expression in transfected 293T cell line (H00007023-T02) by TFAP4 MaxPab polyclonal antibody.Lane 1: TFAP4 transfected lysate(38.70 KDa).Lane 2: Non-transfected lysate.
TFAP4 Antibody (Center) (Cat. #TA324454) western blot analysis in Hela,HL-60 cell line lysates (35ug/lane).This demonstrates the TFAP4 antibody detected the TFAP4 protein (arrow).
TFAP4 Antibody (Center) (Cat. #TA324454) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-TFAP4 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysateTFAP4 is supported by BioGPS gene expression data to be expressed in HepG2
Human Muscle
WB Suggested Anti-TFAP4 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate
Human Brain
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324454 | TFAP4 (Center) antibody |
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| Rabbit polyclonal TFAP4 Antibody (Center) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303773 | TFAP4 |
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| Recombinant protein of human transcription factor AP-4 (activating enhancer binding protein 4) (TFAP4) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401113 | TFAP4 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |