TFPI antibody
Principal name
TFPI antibody
Alternative names for TFPI antibody
Tissue factor pathway inhibitor, TFPI1, LACI, EPI, Lipoprotein-associated coagulation inhibitor, Extrinsic pathway inhibitor
Ncbi ID
9606, NP_001027452.1, NP_001027452, NP_006278, NM_006287, NM_001032281, NP_006278.1, NM_001177319, NP_035706.1, NM_001177320, NM_011576, NM_017200
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of TFPI antibody
- Kit Component:
- - KN202098G1, TFPI gRNA vector 1 in pCas-Guide vector
- KN202098G2, TFPI gRNA vector 2 in pCas-Guide vector
- KN202098D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Enjyoji, K.-I., Emi, M., Mukai, T., Imada, M., Leppert, M. L., Lalouel, J. M., Kato, H.Human tissue
factor pathway inhibitor (TFPI) gene: complete genomic structure and localization on the genetic map of
chromosome 2q.Genomics 17: 423-428, 1993.
2. Kleesiek, K., Schmidt, M., Gotting, C., Brinkmann, T., Prohaska, W.A first mutation in the human
tissue factor pathway inhibitor gene encoding (P151L) TFPI. (Letter)Blood 92: 3976-3977, 1998.
3. Lupu, C., Zhu, H., Popescu, N. I., Wren, J. D., Lupu, F.Novel protein ADTRP regulates TFPI
expression and function in human endothelial cells in normal conditions and in response to
androgen.Blood 118: 4463-4471, 2011.
Western Blot analysis of TFPI expression in transfected 293T cell line (H00007035-T01) by TFPI MaxPab polyclonal antibody.Lane 1: TFPI transfected lysate(33.44 KDa).Lane 2: Non-transfected lysate.
TFPI MaxPab rabbit polyclonal antibody. Western Blot analysis of TFPI expression in mouse liver.
Western Blot analysis of TFPI expression in transfected 293T cell line (H00007035-T02) by TFPI MaxPab polyclonal antibody.Lane 1: TFPI transfected lysate(35.00 KDa).Lane 2: Non-transfected lysate.
HEK293 overexpressing TFPI and probed with TFPI polyclonal antibody ( Cat # PAB6017 ) ( mock transfection in first lane ), tested by Origene.
TFPI polyclonal antibody ( Cat # PAB6017 ) staining ( 0.3 µg/ml ) of HeLa lysate ( RIPA buffer, 35 µg total protein cells per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Representative Standard Curve
Immunohistochemical staining of human colon shows distinct cytoplasmic positivity in endothelium.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm & cytoskeleton (intermediate filaments).This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA: TFPI Antibody
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFPI (RC219033, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFPI.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805999)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805999)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805999)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805999)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805999)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805999)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805999)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805999)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFPI (RC219033, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFPI.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806051)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806051)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFPI (RC219033, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFPI.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806052)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806052)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806052)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFPI (RC219033, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFPI.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFPI (RC219033, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFPI.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806306)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFPI (RC219033, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFPI.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFPI (RC219033, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFPI.
Western blot analysis of extracts of various cell lines, using TFPI antibody.
Immunohistochemistry of paraffin-embedded human liver cancer using TFPI antibody at dilution of 1:100 (200x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using TFPI antibody at dilution of 1:100 (200x lens).
Immunofluorescence analysis of MCF7 cell using TFPI antibody. Blue: DAPI for nuclear staining.
TA302625 staining (0.3µg/ml) of Hela lysate (RIPA buffer, 35µg total protein cells per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Anti-TFPI antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-TFPI antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Western blot analysis of TFPI in rat small intestine tissue lysate with TFPI antibody at 1 ug/mL.
Immunohistochemistry of TFPI in rat small intestine tissue with TFPI antibody at 5 ug/mL.
Immunofluorescence of TFPI in rat small intestine tissue with TFPI antibody at 20 ug/mL.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302625 | TFPI (C-term) antibody |
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| Goat Polyclonal Antibody against Tissue Factor Pathway Inhibitor | |||||||
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Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
